Biological assay methods are still extensively used to estimate the quantity of acetylcholine (ACh) in tissue extracts and perfusates. However, in recent years sensitive gas chromatographic and enzymatic assays have been devised. Since these latter procedures are more specific, they may in the future largely replace the bioassay methods. The major disadvantages of biological assays are (1) substances other than ACh in the extracts or perfusates may evoke a pharmacological response or modify the effects of ACh, and (2) drugs used in such experiments may alter the sensitivity of the test object to ACh. While in many cases these problems can be satisfactorily resolved by changing the extraction or bioassay procedure, certain conditions may preclude the use of bioassay methods. Despite these drawbacks, pharmacological procedures for assaying ACh still enjoy popularity because they are relatively simple to perform and are less expensive.
KeywordsBioassay Method Bathing Fluid Bioassay Procedure Choline Ester Anticholinesterase Agent
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