Advertisement

Smooth Muscle pp 613-621 | Cite as

Measurement of Catecholamines

  • D. M. Paton

Abstract

Many drugs and other procedures produce all or part of their effects on smooth muscle indirectly, by modifying the release of catecholamines from adrenergic nerve terminals. (The loci and mechanisms of action of such drugs are discussed in Part II, Chapter 5) The estimation of the catecholamine contents of smooth muscles and of their perfusates or bathing media is thus frequently an important part of the study of drugs acting on smooth muscle.

Keywords

Cation Exchange Resin Thioglycollic Acid Bathing Medium Liquid Scintillation Spectrometry Sodium Meta Bisulfite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anton, A. H. and Sayre, D. F. 1962. A study of the factors affecting the aluminium oxidetrihydroxyindole procedure for the analysis of catecholamines.Pharmac. Exp. Ther.138: 360–375.Google Scholar
  2. Anton, A. H. and Sayre, D. F. 1968. Possible sources of error in solvent extraction procedures for catecholamines. Eur. J. Pharmac.4:435–440.CrossRefGoogle Scholar
  3. Burnstock, G. 1969. Evolution of the autonomic innervation of visceral and cardiovascular systems in vertebrates. Pharmac. Rev., 21: 247–324.Google Scholar
  4. Engelman, K. and Portnoy, B. 1970. A sensitive double-isotope derivative assay for norepinephrine and epinephrine. Circ. Res., 26:53–57.PubMedCrossRefGoogle Scholar
  5. Engelman, K., Portnoy, B., and Lovenberg, W. 1968. A sensitive and specific double-isotope derivative method for the determination of catecholamines in biological specimens. Am. J. Med. Sci.255:259- 268.PubMedCrossRefGoogle Scholar
  6. Euler, U. S., von and Lishajko, F. 1961. Improved technique for the fluorimetric estimation of catecholamines. Acta Physiol. Scand.51:348–355.CrossRefGoogle Scholar
  7. Gaddum, J. H. and Lembeck, F. 1949. The assay of substances from the adrenal medulla.Br. J. Pharmac., 4:401–408.Google Scholar
  8. Häggendal, J. 1962. On the use of strong exchange resins for determinations of small amounts of catechol amines. Scand. J. Clin. LahInvest.14:537–544.CrossRefGoogle Scholar
  9. Häggendal, J. 1966. Newer developments in catecholamine assay. Pharmac. Rev.18:325–329.Google Scholar
  10. Hughes, J. 1972. Evaluation of mechanisms controlling the release and inactivation of the adrenergic transmitter in the rabbit portal vein and vas deferens. Britt.J.Pharmac.44:472–491.CrossRefGoogle Scholar
  11. Iversen, L. L. 1963. The uptake of noradrenaline by the isolated perfused rat heart. Br. J. Pharmac. Chemother.21:523–537.CrossRefGoogle Scholar
  12. Iversen, L. L. 1967. The Uptake and Storage of Noradrenaline, pp. 5–16, Cambridge University, London.Google Scholar
  13. Iversen, L. L. and Jarrott, B. 1970. Modification of an enzyme radiochemical assay procedure for noradrenaline. Biochem. Pharmac., 19:1841–1843.CrossRefGoogle Scholar
  14. Kopin, I. J. 1968. False adrenergic transmitters. Ann. Rev. Pharmac., 8:377–394.CrossRefGoogle Scholar
  15. Koslow, S. H., Cattabeni, F., and Costa, E. 1972. Norepinephrine and dopamine: assay by mass fragment ography in the picomole range. Science, 176:177–180.PubMedCrossRefGoogle Scholar
  16. Kovacsics, G. B. and Saelens, J. K. 1968. Measurement of the levels and turnover of norepinephrine in discrete areas of rat brain using an enzymic assay. Arch. Int. Pharmacodyn.174:481–490.PubMedGoogle Scholar
  17. Laverty, R. and Taylor, K. M. 1968. The fluorimetric assay of catecholamines and related compounds: improvements and extensions to the hydroxyindole technique. Anal. Biochem., 22:269–279.PubMedCrossRefGoogle Scholar
  18. Mann, M. and West, G. B. 1950. The nature of hepatic and splenic sympathin. Br. J. Pharmac. Chemother., 5:173–177.CrossRefGoogle Scholar
  19. Merrills, R. J. 1962. An autoanalytical method for the estimation of adrenaline and noradrenaline. Nature, 193:988.PubMedCrossRefGoogle Scholar
  20. Nikodijevic, B., Daly, J., and Creveling, C. R. 1969. Catechol-O-methyl transferase. I. An enzymatic assay for cardiac norepinephrine. Biochem. Pharmac.18:1577–1584.CrossRefGoogle Scholar
  21. Roberts, D. J. (with an addendum by Vogt, M.) 1964. Some possible causes of pharmacological activity in blank eluates following the separation of sympathomimetic catecholamines by paper chromatography. J. Pharm. Pharmac.16:313–322.CrossRefGoogle Scholar
  22. Saelens, J. K., Schoen, M. S., and Kovacsics, G. B. 1967. An enzymatic assay for norepinephrine in brain tissue. Biochem. Pharmac.16:1043–1049.CrossRefGoogle Scholar
  23. Schumann, H. J. 1959. Über den Hydroxytyramine Gebalt der Organe. Arch. Exp. Path. Pharmak.236:474–482.CrossRefGoogle Scholar
  24. Sharman, D. F. 1971. Methods of determination of catecholamines and their metabolites. In: Methods and Techniques of Neuro sciences, pp. 83–127. Ed. by Fried, R. Marcel Dekker, New York.Google Scholar
  25. Shaw, F. H. 1938. The estimation of adrenaline. Biochem. J., 32:19–25.PubMedGoogle Scholar
  26. Shote, P. A. and Olin, J. S. 1958. Identification and chemical assay of norepinephrine in brain and other tissues. J. Pharmac. Exp. Ther., 122:295–300.Google Scholar
  27. Udenfriend, S. 1962. Fluoroescence Assay in Biology and Medicine. Academic Press, New York.Google Scholar
  28. Vane, J. R. 1964. The use of isolated organs for detecting active substances in the circulating blood. Br. J. Pharmac.23:360–373.Google Scholar
  29. Vane, J. R. 1966. The estimation of catecholamines by biological assay. Pharmac. Rev.18:317–324.Google Scholar
  30. Vane, J. R. 1969. The release and fate of vaso-active hormones in the circulation. Br. J. Pharmac.35:209- 242.CrossRefGoogle Scholar
  31. Vogt, M. 1971. Bioassay. In: Handbuch, der Exper. Pharmak., 28/2, pp. 108–117.Google Scholar
  32. Weil-Malherbe, H. 1959. The fluorimetric estimation of catechol compounds by the ethylenediamine condensation method. Pharmac. Rev.11:278–288.Google Scholar
  33. Weil-Malherbe, H. 1968. The estimation of total (free + conjugated) catecholamines and some catecholamine metabolites in human urine. In: Methods of Biochemical Analysis, Vol. 16, pp. 293–326. Ed. by Glick, D. Wiley, New York.CrossRefGoogle Scholar
  34. Weil-Malherbe, H. 1971. Determination of catechol amines. In: Analysis of Biogenic Amines and Their Related Enzymes, pp. 119–152. Ed. by Glick, D. Wiley, New York.Google Scholar
  35. Weil-Malherbe, H. and Bone, A. D. 1952. The chemical estimation of adrenaline-like substances in blood. Biochem. J.51:311–318.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • D. M. Paton
    • 1
  1. 1.Department of PharmacologyUniversity of AlbertaEdmontonCanada

Personalised recommendations