Smooth Muscle pp 447-455 | Cite as

Nerve—Muscle Preparations of the Isolated Perfused Spleen

  • H. Thoenen


With the use of experimental methods available so far, only very limited direct information has been gained on events taking place at the adrenergic nerve terminals, where an electrical impulse is transformed into a chemical message which acts on the receptive sites of the effector cells. However, the correlation between the response of an effector organ to nerve stimulation and the amount of transmitter appearing in the venous effluent, together with the modification of these parameters by drugs, provides valuable indirect information on the mechanism of peripheral adrenergic transmission and the site of action of the modifying drugs.


Nerve Stimulation Norepinephrine Concentration Perfusion Fluid Stimulation Rate Splenic Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brown, G. L. 1965. The release and fate of the transmitter liberated by adrenergic nerves. Proc. R. Soc. B, 162:1–19.CrossRefGoogle Scholar
  2. Brown, G. L. and Gillespie, J. S. 1957. The output of sympathetic transmitter from the spleen of the cat. J. Physiol., 138: 81,-102PubMedGoogle Scholar
  3. Burn, J. H. 1952. Practical Pharmacology, pp. 47–50. Blackwell Scientific Publications, Oxford.Google Scholar
  4. Delaunois, A. L., Moerman, E. J., and De Schaepdryver, A. F. 1968. Isolated perfused dog spleen method. Experientia, 24:307–309.PubMedCrossRefGoogle Scholar
  5. Fillenz, M. 1970. The innervation of the spleen. Proc. R. Soc., 174:459–468.CrossRefGoogle Scholar
  6. Fischer, J. E., Horst, W. D., and Kopin, I. J. 1965. β-hydroxylated amines as false neurotransmitters. Br. J. Pharmacol., 24:477–484.Google Scholar
  7. Gewirtz, G. P. and Kopin, I. J. 1970. Release of dopamine β -hydroxylase with norepinephrine during cat splenic nerve stimulation. Nature, 227:406–407.PubMedCrossRefGoogle Scholar
  8. Haefely, W., Huerlimann, A., and Thoenen, H. 1965. Relation between the rate of stimulation and the quantity of noradrenaline liberated from sympathetic nerve endings in the isolated perfused spleen of the cat. J. Physiol, 181:48–58.PubMedGoogle Scholar
  9. Haeggendal, J. 1963. An improved method for fluorimetric determination of small amounts of adrenaline and noradrenaline in plasma and tissues. Acta Physiol. Scand., 59:242–254.PubMedCrossRefGoogle Scholar
  10. Haeusler, G., Haefely, W., and Huerlimann, A. 1969. On the mechanism of the adrenergic nerve blocking action of bretylium. Naunyn-Schmiedebergs Arch. Pharmak., 265:260–277.CrossRefGoogle Scholar
  11. Hedqvist, V. and Stjärne, L. 1969. The relative role of recapture and of de novo synthesis for the maintenance of neurotransmitter homeostasis in noradrenergic nerves. Acta Physiol. Scand., 79:270–283.CrossRefGoogle Scholar
  12. Hertting, G., Suko, J., Widhalm, S., und Harbich, I. 1967. Ueber den Mechanismus der Potenzierung der Katecholaminwirkkung nach chronisch postganglionärer sympathischer Denervierung. Naunyn- Schmiedebergs Arch. Pharmak., 256:40–54.CrossRefGoogle Scholar
  13. Huković, A. und Muscholl, E. 1962. Die Noradrenalinabgabe aus dem isolierten Kaninchenherzen bei sympathischer Nervenreizung und ihre pharmakologische Beeinflussung. Arch. Pharmak. Exp. Path., 244:81–96.CrossRefGoogle Scholar
  14. Kirpekar, S. M. and Misu, Y. 1967. Release of noradrenaline by splenic nerve stimulation and its depend¬ence of calcium. J. Physiol., 188:219–234.PubMedGoogle Scholar
  15. Kirpekar, S. M. and Puig, M. 1971. Effect of flow-stop on noradrenaline release from normal spleen and spleen treated with cocaine, phentolamine or phenoxybezamine. Br. J. Pharmacol., 43:359–369.PubMedCrossRefGoogle Scholar
  16. Kirpekar, S. M., Prat, J. C., and Yamamoto, H. 1970. Effects of metabolic inhibitors on norepinephrine release from the perfused spleen of the cat. J. Pharmacol. Exp. Ther., 172:342–350.PubMedGoogle Scholar
  17. Musacchio, J. M., Fischer, J. E., and Kopin, I. J. 1966. Subcellular distribution and release by sympathetic nerve stimulation of dopamine and α-methyldopamine. J. Pharmacol. Exp. Ther., 152:51–61.PubMedGoogle Scholar
  18. Thoenen, H., Huerlimann, A., and Haefely, W. 1963. The effect of postganglionic sympathetic stimulation on the isolated perfused spleen of the cat: simultaneous determination of norepinephrine output and changes in volume and vascular resistance. Helv. Physiol. Acta, 21:17–26.Google Scholar
  19. Thoenen, H., Huerlimann, A., and Haefely, W. 1964a. The effect of sympathetic nerve stimulation on volume, vascular resistance, and norepinephrine output in the isolated perfused spleen of the cat, and its modification by cocaine.- J. Pharmacol. Exp. Ther., 143:57–63.PubMedGoogle Scholar
  20. Thoenen, H., Huerlimann, A., und Haefely, W. 1964b. Wirkungen von Phenoxybenzamin, Phentolamin und Azapetin auf adrenergische Synapsen der Katzenmilz. Helv. Physiol. Acta, 22:148–161.Google Scholar
  21. Thoenen, H., Huerlimann, A., and Haefely, W. 1966a. Interaction of phenoxbenzamine with guanethidine and bretylium at the sympathetic nerve endings of the isolated perfused spleen of the cat. J. Pharmacol. Exp. Ther., 151:189–195.PubMedGoogle Scholar
  22. Thoenen, H., Tranzer, J. P., Huerlimann, A., and Haefely, W. 1966b. Untersuchungen zur Frage eines cholinergischen Gliedes in der postganglionären sympathischen Transmission. Helv. Physiol. Acta, 24:229–246.Google Scholar
  23. Thoenen, H., Haefely, W., Gey, K. F., and Huerlimann, A. 1967a. Diminished effect of sympathetic nerve stimulation in cats pretreated with 5-hydroxydopa: formation and liberation of false adrenergic transmitters. Naunyn-Schmiedebergs Arch. Pharmak. Exp. Pathol., 259:17–33.CrossRefGoogle Scholar
  24. Thoenen, H., Haefely, W., and Staehelin, H. 1967b. Potentiation by tetraethylammonium of the response of the cat spleen to postganglionic sympathetic nerve stimulation. J. Pharmacol. Exp. Ther., 157:532–540.PubMedGoogle Scholar
  25. Thoenen, H., Haefely, W., Haeusler, G., and Huerlimann, A. 1968. Formation of a “false” adrenergic transmitter in cats pretreated with 4-methoxy-3,5-dihydroxyphenylalanine and its effects on postganglionic sympathetic transmission. J. Pharmacol. Exp. Ther., 162:70–79.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • H. Thoenen
    • 1
  1. 1.Department of PharmacologyBiocenter of the UniversityBaselSwitzerland

Personalised recommendations