Smooth Muscle pp 403-417 | Cite as

Nerve—Muscle Preparations of Vas Deferens

  • Mollie E. Holman


In 1961 Huković described an isolated preparation of the guinea pig vas deferens which responded to repetitive stimulation of the hypogastric nerve with “quick and strong” contractions which were reproducible for many hours. Since this smooth muscle is not normally spontaneously active and does not appear to have an inhibitory innervation, it seemed that the hypogastric nerve—vas deferens preparation might be a most useful tool for studies on the transmission of excitation from autonomic nerves to smooth muscle.


Ganglion Cell Terminal Axon Hypogastric Nerve Pelvic Plexus Preganglionic Fiber 
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  1. Ambache, N. and Zar, M. Aboo. 1971. Evidence against adrenergic motor transmission in the guinea pig vas deferens. J. Physiol, 216:359–389.PubMedGoogle Scholar
  2. Anstey, M. D. 1972. The circular muscle of the vas deferens of the guinea pig. J. Physiol., 222:81P.Google Scholar
  3. Bell, C. and McLean, J. R. 1967. Localization of norepinephrine and acetylcholinesterase in separate neurons supplying the guinea pig vas deferens. Br. J. Pharmac. Chemother., 31:276–289CrossRefGoogle Scholar
  4. Bennett, M. R. 1972. Autonomic Neuromuscular Transmission. Monographs of the Physiological Society. Cambridge University Press.Google Scholar
  5. Bentley, G. A. 1972. Pharmacological studies on the hypogastric ganghon of the rat and guinea pig. Br. J. Pharmacol., 44:392–509.CrossRefGoogle Scholar
  6. Bentley, G. A. and Smith, G. 1967. Effects of alpha-adrenergic receptor blocking drugs on the response of the vas deferens and arterial smooth muscle to sympathetic drugs and stimulation. Circ. Res. (Suppl. III), 20:101–109.Google Scholar
  7. Birmingham, A. J. and Wilson, A. B. 1963. Preganglionic and postganglionic stimulation of the guinea pig isolated vas deferens preparation. Br. J. Pharmac. Chemother., 27:569–580.CrossRefGoogle Scholar
  8. Brites, G. 1931. Sur la tunique musculaire du canal deferent de I’adult. Folia anat., U. Couimbr. 6:1–50.Google Scholar
  9. Burn, J. H. and Rand, M. J. 1960. The relation of circulating noradrenaline to the effect of sympathetic stimulation. J.’Physiol., 750:295–305.Google Scholar
  10. Burnstock, G. 1970. Structure of smooth muscle and its innervation. In: Smooth Muscle, pp. 1–69. Ed. by Bülbring, E. Brading, A. Jones, A. and Tomita, T. Edward Arnold, London.Google Scholar
  11. Burnstock, G. and Holman, M. E. 1961. The transmission of excitation from autonomic nerve to smooth muscle. J. Physiol, 755:115–133.Google Scholar
  12. Crowcroft, P. J. and Szurszewski, J. H. 1971. A study of the inferior mesenteric and pelvic ganglia of guinea pigs with intracellular electrodes. J. Physiol, 279:421–441.Google Scholar
  13. Eränkö, O. and Eränkö, L. 1971. Small intensely fluorescent granule-containing cells in the sympathetic ganglia of the rat. Prog. Brain Res., 34:39–51.CrossRefGoogle Scholar
  14. Falck, B., Owman, Ch.,andSjöstrand, N. O.1965. Peripherally located adrenergic neurons innervating vas deferens and seminal vesical of the guinea pig. Experientia, 27:98–100.CrossRefGoogle Scholar
  15. Farnebo, L-O. and Malmfors, T. 1971. 3H-noradenaline release and mechanical response in the field stimulated mouse vas deferens. Acta Physiol Scand., 371:1–18.CrossRefGoogle Scholar
  16. Ferry, C. B. 1967. The innervation of the vas deferens of the guinea pig. J. Physiol, 192:463–478.PubMedGoogle Scholar
  17. Furness, J. B. 1970. The effect of external potassium ion concentration on autonomic neuromuscular transmission. Pflüger s Arch., 317:310–326.CrossRefGoogle Scholar
  18. Gruber, M. 1933. The autonomic innervation of the genito-urinary tract. Physiol Rev., 13: 497–609.Google Scholar
  19. Haefely, W. 1969. Effects of catecholamines in the cat superior cervical ganglion and their postulated role as physiological modulators of ganglionic transmission. Prog. Brain. Res., 57:61–72CrossRefGoogle Scholar
  20. Haefely, W. 1972. Electrophysiology of the adrenergic neuron. In: Handbook of Experimental Pharmacology. Ed. by Eichler, O., Herken, H., and Welch, A. D. Vol. XXXIII, Ed. by Blaschlo, H. and Muscholl, E. pp. 661–725. Springer-Verlag.Google Scholar
  21. Holman, M. E. 1970. Junction potentials in smooth muscle. In: Smooth Muscle, pp. 244–288. Ed. by Bülbring, E., Brading, A., Jones, A., and Tomita, T. Edward Arnold, London.Google Scholar
  22. Hotta, Y. 1969. Some properties of the junctional and extrajunctional receptors in the vas deferens of the guinea pig. Agents and Actions, 1:13–21.PubMedCrossRefGoogle Scholar
  23. Hukovic, S. 1961. Responses of the guinea pig vas deferens. Br. J. Pharmacol, 16:188–194.Google Scholar
  24. Jacobowitz, D. 1970. Catecholamine fluorescence studies of adrenergic neurons and chromaffin cells in sympathetic ganglia. Fed. Proc.29:1929–1944.PubMedGoogle Scholar
  25. Jacobowitz, D. and Woodward, J. K. 1968. Adrenergic neurons in the cat superior cervical ganglion and cervical sympathetic nerve trunk. A histochemical study. J. Pharmacol Exp. Ther., 762:213–226.Google Scholar
  26. Katz, B. 1966. Nerve, Muscle and Synapse. McGraw-Hill, New York.Google Scholar
  27. Langley, J. N. and Anderson, H. K. 1894. The constituents of the hypogastric nerves. Physiol, 17: 177–191.Google Scholar
  28. Langley, J. N. and Anderson, H. K. 1896. The innervation of the pelvic and adjoining viscera. Part IV. The internal generative organs. J. Physiol, 19:122–130.Google Scholar
  29. Libet, B. and Tosaka, T. 1970. Dopamine as a synaptic transmitter and modulator in sympathetic ganglia: a different mode of synaptic action. Prod Nat. Acad. Sci., 67:667–673.CrossRefGoogle Scholar
  30. Martin, A. R. 1966. Quantal nature of synaptic transmission. Physiol. Rev. 46:51–66.Google Scholar
  31. Matthews, R. and Raisman, G. 1969. The ultrastructure and somatic efferent synapses of small granule- containing cells in the superior cervical ganglion. J. Anat., 705:255–282.Google Scholar
  32. Merrillees, N. C. R. 1968. The nervous environment of individual smooth muscle cells of the guinea pig vas deferens. J. Cell. Biol., 79:529–550.Google Scholar
  33. Norberg, K.-A. and Sjöqvist, F. 1966. New possibilities for adrenergic modulation of ganglionic transmission. Pharmacol. Revs., 18:743–751.Google Scholar
  34. Ohlin, P. and Strömblad, B. C. R. 1963. Observations on the isolated vas deferens. Br. J. Pharmacol. 20:299–306.Google Scholar
  35. Olson, L. and Malmfors, T. 1970. Growth characteristics of adrenergic nerves in the adult rat. Acta. Physiol. Scand. (Suppl. 348), 7–112.Google Scholar
  36. Ostberg, A. 1971. Catecholamine storing organelles. Ph.D. Thesis, Monash University.Google Scholar
  37. Owman, Ch. and Sjöstrand, N. O. 1965. Short adrenergic neurons and catecholamine-containing cells in vas deferens and accessory male genital glands of different mammals. Z. Zellforsch., 66:300–320.PubMedCrossRefGoogle Scholar
  38. Paton, W. D. M. 1955. The response of the guinea pig ileum to electrical stimulation by coaxial electrodes. J. Physiol., 727:40–41 P.Google Scholar
  39. Revel, J. P., Olson, W., and Karnovsky, M. J. 1967. A twenty-Angström gap junction with hexagonal array of subunits in smooth muscle. J. Cell. Biol., 35:112A.Google Scholar
  40. Sjöstrand, N. O. 1962. Effect of reserpine and hypogastric denervation on the noradrenaline content of the vas deferens and the seminal vesicle of the guinea pig. Acta Physiol. Scand., 56:376–380.PubMedCrossRefGoogle Scholar
  41. Sjöstrand, N. O. 1965. The adrenergic innervation of the vas deferens and the accessory male genital glands. An experimental and comparative study of its anatomical and functional organization in some mammals, including the presence of adrenaline and chromaffin cells in these organs. Acta Physiol. Scand., 65(Suppl. 257): 1–82.Google Scholar
  42. Swedin, G. 1971. Studies on neuromuscular transmission mechanisms in the rat and guinea pig vas deferens. Acta Physiol. Scand. (Suppl. 369), 1–34.Google Scholar
  43. Tomita, T. 1966. Electrical responses of smooth muscle to external stimulation in hypertonic solution. J. Physiol, 755:450–468.Google Scholar
  44. Tomita, T. 1967. Current spread in the smooth muscle of the guinea pig vas deferens. J. Physiol, 189:163–176.PubMedGoogle Scholar
  45. Tranzer, J. P. and Thoenen, H. 1968. Various types of amine-storing vesicles in peripheral adrenergic nerve terminals. Experientia, 24:484–486.PubMedCrossRefGoogle Scholar
  46. Trendelenburg, U. 1967. Some aspects of the pharmacology of autonomic ganglion cells. Ergebn. Physiol, 59:1–85.Google Scholar
  47. Van Orden, L. S., Burke, J. P., Geymer, M., and Lodoen, F. V. 1970. Localisation of depletion-sensitive and depletion-resistant norepinephrine storage sites in autonomic ganglia. J. Pharmacol. Exp. Ther., 774:56–71.Google Scholar
  48. Voile, R. L. 1969. Ganghonic transmission. Ann. Rev. Pharmacol, 9:135–146.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Mollie E. Holman
    • 1
  1. 1.Department of PhysiologyMonash UniversityClaytonAustralia

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