Studies of Soluble Transplantation and Tumor Antigens
Considerable progress has been made in the last 15 years in solubilizing and purifying transplantation antigens (H-2) of the mouse. Until recently, adequate biological assays for activity of solubilized materials were not performed; thus activity was measured principally in terms of inhibition of complementdependent cytotoxicity of lymphoid cells or inhibition of hemagglutination using specially prepared antisera against known determinants. Neither assay is a measure of completeness of antigen; haptenic or nonantigenic fragments may be detected as well as definitive determinants. Recently, newer methods—for example, the use of nonionic detergents—have been used to solubilize H-2 antigens; these so far have precluded adequate biological testing because of the nature of the solubilized material.
KeywordsTumor Antigen Tumor Rejection Soluble Antigen Crude Membrane Transplantation Antigen
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