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The Molecular Biology of RNA Tumor Viruses

  • J. Michael Bishop
  • Harold E. Varmus
Part of the Cancer book series (C, volume 2)

Abstract

RNA tumor viruses are useful tools for the study of oncogenesis because they rapidly induce tumors in animals and efficiently transform cells in culture. These viruses are distinguished by certain morphological features (Bernhard, 1960; Sarker et al., 1971a), an exceptionally large single-stranded RNA genome (about 30,000 nucleotides, Duesberg, 1970) and an RNA-directed DNA polymerase which transcribes the viral genome into single- and double-stranded DNA (Baltimore, 1970; Temin and Mizutani, 1970; Temin and Baltimore, 1972). This transcription, the mechanism by which it occurs, the fate of its products in the infected cell, and the role of the products in both the viral life cycle and virus-induced transformation of the host cell are the principal subjects of our discussion.

Keywords

Mouse Mammary Tumor Virus Rous Sarcoma Virus Permissive Cell Avian Myeloblastosis Virus Avian Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aaronson, S. A., and Dunn, C. Y., 1973, High-frequency C-type virus induction by inhibitors of protein synthesis, Science 183: 422.Google Scholar
  2. Aaronson, S. A., Todaro, G. J., and Scolnick, E. M., 1971, Induction of murine C-type viruses from clonal lines of virus-free BALB/3T3 cells, Science 174: 157.PubMedGoogle Scholar
  3. Adesnik, M., Salditt, M., Thomas, W., and Darnell, J. E., 1972, Evidence that all messenger RNA molecules (except histone messenger RNA) contain poly(A) sequences and that the poly(A) has a nuclear function, J. Mol. Biol. 71:21. Google Scholar
  4. Altaner, C., and Temin, H. M., 1970, Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitroby avian sarcoma viruses, Virology 40: 118.PubMedGoogle Scholar
  5. Arndt-Jovin, D. J., and Berg, P., 1971, Quantitative binding of 125I-concanavalin A to normal and transformed cells, J. Virol. 8: 716.PubMedGoogle Scholar
  6. Bader, A. V., 1973, Role of mitochondria in the production of RN A-containing tumor viruses, J. Virol. 11: 314.PubMedGoogle Scholar
  7. Bader, J. P., 1965, The requirement for DNA synthesis in the growth of Rous sarcoma and Rous-associated viruses, Virology 26: 253.PubMedGoogle Scholar
  8. Bader, J. P., 1966, Metabolic requirements for infection by Rous sarcoma virus. I. Transient requirement for DNA synthesis, Virology 29: 444.PubMedGoogle Scholar
  9. Bader, J. P., 1972a, Metabolic requirements for infection by Rous sarcoma virus. IV. Virus reproduction and cellular transformation without cellular division, Virology 48: 494.Google Scholar
  10. Bader, J. P., 19726, Temperature-dependent transformation of cells infected with a mutant of Bryan Rous sarcoma virus, J. Virol. 10:267.Google Scholar
  11. Bader, J. P., 1973, Virus-induced transformation without cell division, Science 180: 1069.PubMedGoogle Scholar
  12. Bader, J. P., and Steck, T. L., 1969, Analysis of the ribonucleic acid of murine leukemia virus, J. Virol. 4: 454.PubMedGoogle Scholar
  13. Balduzzi, P., and Morgan, H. R., 1970, Mechanism of oncogenic transformation by Rous sarcoma virus. I. Intracellular inactivation of cell-transforming ability of Rous sarcoma virus by 5-bromodeoxyuridine and light, J. Virol. 5: 470.PubMedGoogle Scholar
  14. Baltimore, D., 1970, RNA-dependent DNA polymerase in virions of RNA tumor viruses, Nature (Lond.) 226: 1209.Google Scholar
  15. Baltimore, D., 1971a, Expression of animal virus in genomes, Bacteriol. Rev. 35: 235.Google Scholar
  16. Baltimore, D., 19716, Is poliovirus dead? in: Perspectives in Virology, Vol. VII (M. Pollard, ed.), pp. 1–14, Academic Press, New York.Google Scholar
  17. Baltimore, D., and Smoler, D. F., 1971, Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses (avian myeloblastosis virus/mouse leukemia virus/E. coliDNA polymerase/homopolynucleotides/oligonucleotides), Proc. Natl. Acad. Sci. 68: 1507.PubMedGoogle Scholar
  18. Baltimore, D., and Smoler, D. F., 1972, Association of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase, J. Biol. Chem. 247: 7282.PubMedGoogle Scholar
  19. Baltimore, D., Verma, I. M., Drost, S., and Mason, W. S., 1974, Temperature sensitive DNA polymerase from Rous sarcoma virus mutants, Cancer 34: 1395.PubMedGoogle Scholar
  20. Baluda, M. A., 1972, Widespread presence, in chickens, of DNA complementary to the DNA genome of avian leukosis viruses, Proc. Natl. Acad. Sci. 69: 576.PubMedGoogle Scholar
  21. Baxt, W. G., and Spiegelman, S., 1973, Nuclear DNA sequences present in human leukemic cells and absent in normal leukocytes, Proc. Natl. Acad. Sci. 70: 627.Google Scholar
  22. Beeman, K., Duesberg, P., and Vogt, P., 1974, Evidence for crossing-over between avian tumor viruses based on analysis of viral RNAs, Proc. Natl. Acad. Sci. USA 71: 4254.Google Scholar
  23. Bentvelzen, P., Daams, J. H., Hageman, P., and Calafat, J., 1970, Genetic transmission of viruses that incite mammary tumors in mice, Proc. Natl. Acad. Sci. 67: 377.PubMedGoogle Scholar
  24. Benveniste, R. E., Todaro, G. J., Scolnick, E. M., and Parks, W. P., 1973, Partial transcription of murine type C viral genomes in BALB/c cell lines, J. Virol. 12: 711.PubMedGoogle Scholar
  25. Benveniste, R. E., Lieber, M. M., Livingston, D. M.,Shen,C. J., and Todaro, G.J., 1974, Infectious type C virus isolated from a baboon placenta, Nature (Lond.) 248:17. Google Scholar
  26. Berkower, I., Leis, J., and Hurwitz, J., 1973, Isolation and characterization of an endonuclease from Escherichia colispecific for ribonucleic acid in ribonucleic acid deoxyribonucleic acid hybrid structures,/. Biol. Chem. 248: 5914.Google Scholar
  27. Bernhard, W., 1960, The detection and study of tumor viruses with the electron microscope, Cancer Res. 20: 712.PubMedGoogle Scholar
  28. Biggs, P. M., Milne, B. S., Graf, T., and Bauer, H., 1973, Oncogenicity of nontransforming mutants of avian sarcoma viruses, J. Gen. Virol. 18: 399.PubMedGoogle Scholar
  29. Billeter, M. A., Parsons, J. T., and Coffin, J. M., 1974, The nucleotide sequence complexity of avian tumor virus RNA, Proc. Natl. Acad. Sci. USA 71: 3560.PubMedGoogle Scholar
  30. Bishop, J. M., Levinson, W. E., Quintrell, N., Sullivan, D., Fanshier, L., and Jackson, J., 1970a, The low molecular weight RNAs of Rous sarcoma virus. I. The 4S RNA, Virology 42: 182.Google Scholar
  31. Bishop, J. M., Levinson, W. E., Sullivan, D., Fanshier, L., Quintrell, N., and Jackson, J., 19706, The low molecular weight RNAs of Rous sarcoma virus. II. The 7S RNA, Virology 42:927.Google Scholar
  32. Bishop, J. M., Faras, A. J., Garapin, A. C., Hansen, C., Jackson, N., Levinson, W. E., Taylor, J. M., and varmus, H. E., 1973, RNA-directed DNA polymerase and the replication of Rous sarcoma virus, in: Molecular Studies in Viral Neoplasia(25th Annual M. D. Anderson Symposium on Fundamental Cancer Research, 1972, R. W. Cumley, ed.), University of Texas Press, Austin, pp. 229–254.Google Scholar
  33. Bishop, J. M., Quintrell, N., Medeiros, E., and Varmus, H. E., 1974a, Of birds and mice and men: Comments on the use of animal models and molecular hybridization in the search for human tumor viruses, Cancer 34: 1421.Google Scholar
  34. Bishop, J. M., Quintrell, N., Medeiros, E., and Varmus, H. E., 1974a, Of birds and mice and men: Comments on the use of animal models and molecular hybridization in the search for human tumor viruses, Cancer 34: 1421.Google Scholar
  35. Biswal, N., Mccain, B., and Benyesh-Melnick, M., 1971, The DNA of murine sarcoma leukemia virus, Virology 45: 697.PubMedGoogle Scholar
  36. Boettiger, D., 1974, Reversion and induction of Rous sarcoma virus expression in virus-transformed baby hamster kidney cells, Virology 62: 522.PubMedGoogle Scholar
  37. Boettiger, D., and Temin, H. M., 1970, Light inactivation of focus formation by chicken embryo fibroblasts infected with avian sarcoma virus in the presence of 5-bromodeoxyuridine, Nature (Lond.) 228: 622.Google Scholar
  38. Bolognesi, D. P., and Bauer, H., 1970, Polypeptides of avian RNA tumor viruses. I. Isolation and physical and chemical analysis, Viriology 42: 1097.Google Scholar
  39. gg Bolognesi, D. P., Bauer, H., Gelderblom, H., and Huper, G., 1972a, Polypeptides of avian RNA tumor viruses. IV. Components of the viral envelope, Virology 47: 551.Google Scholar
  40. J. M. Bishop Bolognesi, D. P., Gelderblom, H., Bauer, H., Molling, k., and Huper, G., 19726, Polypeptides of AND avian RNA tumor viruses. V. Analysis of the virus core, Virology 47:567.Google Scholar
  41. H. E. Varmus Bonar, R. A., Sverak, L., Bolognesi, D. P., Langlois, A. J., Beard, D., and Beard, J. W., 1967, Ribonucleic acid components of BAI strain A (myeloblastosis) avian tumor virus, Cancer Res. 27: 1138.PubMedGoogle Scholar
  42. Buck, C. A., Glick, M. C., and Warren, L., 1970, A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells, Biochemistry 9: 4567.PubMedGoogle Scholar
  43. Buck, C. A., Glick, M. C., and Warren, L., 1971a, Glycopeptides from the surface of control and virus-transformed cells, Science 172: 169.Google Scholar
  44. Buck, C. A., Glick, M. C., and Warren, L., 19716, Effect of growth on the glycoproteins from the surface of control and Rous sarcoma virus transformed cells, Biochemistry 10:2176.Google Scholar
  45. Burger, M. M., 1969, A difference in the architecture of the surface membrane of normal and virally-transformed cells, Proc. Natl. Acad. Sci. 62: 994.PubMedGoogle Scholar
  46. Burger, M. M., and Martin, G. S., 1972, Agglutination of cells transformed by Rous sarcoma virus by wheat germ agglutinin and concanavalin A, Nature New. Biol. 237: 9.PubMedGoogle Scholar
  47. Burger, M. M., and Noonan, K. D., 1970, Restoration of normal growth by covering of agglutinin sites on tumor cell surface, Nature (Lond.) 228: 512.Google Scholar
  48. Canaani, E., and Duesberg, P., 1972, Role of subunits of 60 to 70S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase, J. Virol. 10: 23.PubMedGoogle Scholar
  49. Canaani, E., Helm, K. V. D., and Duesberg, P., 1973, Evidence for 30–40S RNA as precursor of the 60–70S RNA of Rous sarcoma virus, Proc. Natl. Acad. Sci. 72: 401.Google Scholar
  50. Carchman, R. A., Johnson, G. S., Pastan, I., and Scolnick, E. M., 1974, Studies on the levels of cyclic AMP in cells transformed by wild-type and temperature-sensitive Kirsten sarcoma virus, Cell 1: 59.Google Scholar
  51. Cheung, K.-S., Smith, R. E., Stone, M. P., and Joklik, W. K., 1972, Comparison of immature (rapid harvest) and mature Rous sarcoma virus particles, Virology 50: 851.PubMedGoogle Scholar
  52. Clarke, G. D., Stoker, M. G. P., Ludlow, A., and Thornton, M., 1970, Requirement of serum for DNA synthesis in BHK 21 cells: Effects of density, suspension and virus transformation, Nature (Lond.) 227: 798.Google Scholar
  53. Cline, M. J., and Livingston, D. C., 1971, Concanavalin A—Role in agglutinating transformed cells, Nature New Biol. 232: 155.PubMedGoogle Scholar
  54. Coffin, J. M., 1972, Rescue of Rous sarcoma virus from Rous sarcoma virus transformed mammalian cells,/. Virol. 10: 153.Google Scholar
  55. Coffin, J. M., and Temin, H. M., 1972, Hybridization of Rous sarcoma virus deoxyribonucleic acid polymerase product and ribonucleic acid from chicken and rat cells infected with Rous sarcoma virus, J. Virol. 9: 766.PubMedGoogle Scholar
  56. Crittenden, L. B., 1968, Observations on the nature of genetic cellular resistance to avian tumor viruses, J. Natl. Cancer Inst. 41: 145.PubMedGoogle Scholar
  57. Dahlberg, J. E., Sawyer, R. C., Taylor, J. M., Faras, A. J., Levinson, W. E., Goodman, H. M., and Bishop, J. M., 1974, Transcription of DNA from the 70S RNA of Rous sarcoma virus: Identification of a specific 4S RNA which serves as primer, J. Virol. 13: 1126.PubMedGoogle Scholar
  58. Dales, S., and Hanafusa, H., 1972, Penetration and intracellular release of the genomes of avian RNA tumor viruses, Virology 50: 440.PubMedGoogle Scholar
  59. Davis, N. L., and Rueckert, R. R., 1972, Properties of a ribonucleoprotein particle isolated from Nonidet P-40-treated Rous sarcoma virus, J. Virol. 10: 1010.PubMedGoogle Scholar
  60. Deng, C. T., Boettiger, D., Macpherson, I., and Varmus, H. E., 1974, The persistence and expression of virus-specific DNA in revertants of Rous sarcoma virus-transformed BHK-21 cells, Virology 62: 512.PubMedGoogle Scholar
  61. Thé, G., Becker, C., and Beard, J. W., 1964, Virus of avian myeloblastosis (BAI strain A). XXV. Ultracytochemical study of virus and myeloblast phosphatase activity, J. Natl. Cancer Inst. 32: 201.Google Scholar
  62. Duesberg, P. H., 1968, Physical properties of RSV RNA, Proc. Natl. Acad. Sci. 60: 1511.PubMedGoogle Scholar
  63. Duesberg, P. H., 1970, On the structure of RNA tumor viruses, Curr. Top. Microbiol. Immunol. 51: 79.Google Scholar
  64. Duesberg, P. H., and Vogt, P. K., 1970, Differences between the ribonucleic acids of transforming and nontransforming avian tumor viruses, Proc. Natl. Acad. Sci. 67: 1673.PubMedGoogle Scholar
  65. Duesberg, P. H., and Vogt, P. K., 1973a, Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: Comparison of other viral and cellular RNA species, J. Virol. 12: 594.Google Scholar
  66. Duesberg, P. H., and Vogt, P. K., 19736, RNA species obtained from clonal lines of avian sarcoma and avian leukosis virus, Virology 54:207.Google Scholar
  67. Duesberg, P. H., Martin, G. S., and Vogt, P. K., 1970, Glycoprotein components of avian and murine RNA tumor viruses, Virology 41: 631.PubMedGoogle Scholar
  68. Duesberg, P., Helm, K. V. D., and Canaani, E., 1971, Comparative properties of RNA and DNA templates for the DNA polymerase of Rous sarcoma virus, Proc. Natl. Acad. Sci. 68: 2505.PubMedGoogle Scholar
  69. Duesberg, P. H., Vogt, P. K., and Martin, G. S., 1973a, The 60–70S RNA of avian sarcoma and leukosis viruses: Distribution of class a and b subunits, in: Unifying Concepts of Leukemia (R. M. Dutcher, ed.), Bibl. Haematol. 99: 462–473.Google Scholar
  70. Duesberg, P. H., Vogt, P. K., Maisel, J., Lai, M. M.-C, and Canaani, E., 19736, Tracking defective tumor virus RNA, in: Virus Research (Proceedings of the Second ICN-UCLA Symposium on Molecular Biology, C. F. Fox and W. S. Robinson, eds.), pp. 327–338, Academic Press, New York and London.Google Scholar
  71. Dulbecco, R., 1970, Topoinhibition and serum requirement of transformed and untransformed cells, Nature (Lond.) 227: 802.Google Scholar
  72. Dulbecco, R., and Elkington, J., 1973, Conditions limiting multiplication of fibroblastic and epithelial cells in dense cultures, Nature (Lond.) 246: 197.Google Scholar
  73. East, J. L., Allen, P. T., Knesek, J. E., Chan, J. C., Bowen, J. M., and DmochowskiL., 1973, Structural rearrangement and subunit composition of RNA from released Soehner-Dmochowski murine sarcoma virions, J. Virol. 11: 709.PubMedGoogle Scholar
  74. Elder, K. T., and Smith, A. E., 1973, Methionine transfer ribonucleic acids of avian myeloblastosis virus, Proc. Natl. Acad. Sci. 70: 2823.PubMedGoogle Scholar
  75. Elder, K. T., and Smith, A. E., Methionine transfer RNAs associated with avian oncornavirus 70S RNA, Nature (Lond.) 247:435.Google Scholar
  76. Ellerman, V., and Bang, O., 1909, Experimentelle Leukämie bei Huhnern, Z. Hyg. Infekt. 62: 231.Google Scholar
  77. Emanoil-Ravicovitch, R., Larsen, C. J., Bazifier, M., Robin, J., Peries, J., and Boiron, M., 1973, Low molecular weight RNAs of murine sarcoma virus: Comparative studies of free and 70S RNA-associated components, J. Virol. 12: 1625.PubMedGoogle Scholar
  78. Erikson, E., and Erikson, R. L., 1970, Isolation of amino acid acceptor RNA from purified avian myeloblastosis virus, J. Mol. Biol.52: 387.PubMedGoogle Scholar
  79. Erikson, E., and Erikson, R. L., 1971, Association of 4S ribonucleic acid with oncornavirus ribonucleic acids, J. Virol. 8: 254.PubMedGoogle Scholar
  80. Erikson, E., Erikson, R. L., Henry, B., and Pace, N. R., 1973, Comparison of oligonucleotides produced by RNase T1 digestion of 7S RNA from avian and murine oncornaviruses and from uninfected cells, Virology 53: 40.PubMedGoogle Scholar
  81. Erikson, R. L., 1969, Studies on the RNA from avian myeloblastosis virus, Virology 37: 124.PubMedGoogle Scholar
  82. Fan, H, and Baltimore, D., 1973, RNA metabolism of murine leukemia virus: Detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA, J. Mol. Biol. 80: 93.PubMedGoogle Scholar
  83. Fan, H, and Paskind, M., 1974, Measurement of the sequence complexity of cloned Moloney murine leukemia virus 60–70S RNA: Evidence for a haploid genome, J. Virol. 14: 421.PubMedGoogle Scholar
  84. Faras, A. J., Taylor, J. M., Mcdonnell, J. P., Levinson, W. E., and Bishop, J. M., 1972, Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus, Biochemistry 11: 2334.PubMedGoogle Scholar
  85. Faras, A. J., Garapin, A. C., Levinson, W. E., Bishop, J. M., and Goodman, H. M., 1973a, Characterization of low molecular weight RNAs associated with the 70S RNA of Rous sarcoma virus, J. Virol. 12: 334.Google Scholar
  86. Faras, A. J., Taylor, J. M., Levinson, W. E., Goodman, H. M., and Bishop, J. M., 19736, RNA-directed DNA polymerase of Rous sarcoma virus: Initiation of synthesis with 70S viral RNA as template, J. Mol. Biol. 79:163.Google Scholar
  87. Faras, A. J., Dahlberg, J. E., Sawyer, R. C., Harada, F., Taylor, J. M., Levinson, W. E., and Bishop, J. M., 1974, Transcription of DNA from the 70S RNA of Rous sarcoma virus: Structure of a 4S RNA primer,/. Virol. 13: 1134.Google Scholar
  88. Fleissner, E., 1971, Chromatographic separation and antigenic analysis of proteins of the oncornvir-uses. I. Avian leukemia-sarcoma viruses, J. Virol. 8: 778.PubMedGoogle Scholar
  89. Fraumeni, J. F., and Miller, R. W., 1966, Epidemiology of human leukemia: Recent observations, J. Natl. Cancer Inst. 38: 593.Google Scholar
  90. Frearson, P. M., and Crawford, L. V., 1972, Polyoma virus basic proteins, J. Gen. Virol. 14: 141.PubMedGoogle Scholar
  91. Freeman, A. E., Kelloff, G. J., Gilden, R. V., Lane, W. T., Swain, A. P., and Huebner, R. J., 1971, Activation and isolation of hamster-specific C-type RNA viruses from tumors induced by cell cultures transformed by chemical carcinogens, Proc. Natl. Acad. Sci. 68: 2386.PubMedGoogle Scholar
  92. Gallagher, R. E., and Gallo, R. C., 1973, Chromatographic analyses of isoaccepting tRNAs from avian myeloblastosis virus, J. Virol. 12: 449.Google Scholar
  93. Gallo, R. C., Miller, N. R., Saxinger, W. C., and Gillespie, D., 1973, Primate RNA tumor virus-like DNA synthesized endogenously by RNA-dependent DNA polymerase in virus-like particles from fresh human acute leukemic blood cells, Proc. Natl. Acad. Sci. 70: 3219.PubMedGoogle Scholar
  94. Garapin, A. C., Varmus, H. E., Faras, A. J., Levinson, W. E., and Bishop, J. M., 1973, RNA-directed DNA synthesis by virions of Rous sarcoma virus: Further characterization of the templates and the extent of their transcription, Virology 52: 264.PubMedGoogle Scholar
  95. Geering, G., Aoki, T., and Old, L., 1970, Shared viral antigen of mammalian leukemia viruses, Nature (Lond.) 226: 265.Google Scholar
  96. Gelb, L. D., Aaronson, S. A., and Martin, M. A., 1971, Heterogeneity of murine leukemia virus in vitroDNA; detection of viral DNA in mammalian cells, Science 172: 1353.PubMedGoogle Scholar
  97. Gelderblom, H., Bauer, H., and Graf, T., 1972, Cell-surface antigens induced by avian RNA tumor viruses: Detection by immunoferritin technique, Virology 47:416.Google Scholar
  98. Gielkens, A. L. G., Saldem, M. H. L., Bloemendal, H., and Konigs, R. N. H., 1972, Translation of oncogenic viral RNA and eukaryotic messenger RNA in the E. colicell-free system, FEBS Letters 28: 348.PubMedGoogle Scholar
  99. Gilden, R. V., Oroszlan, S., and Huebner, R. J., 1971, Coexistence of intraspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses, Nature New. Biol. 231: 107.PubMedGoogle Scholar
  100. Graf, T., 1972, A plaque assay for avian RNA tumor viruses, Virology 50: 567.PubMedGoogle Scholar
  101. Graf, T., and Friis, R. R., 1973, Differential expression of transformation in rat and chicken cells infected with an avian sarcoma virus ts mutant, Virology 56: 369.PubMedGoogle Scholar
  102. Granboulan, N., Huppert, J., and Lacour, F., 1966, Examen au microscope electronique du RNA du virus de la myeloblastose aviaine, J. Mol. Biol. 16: 571.PubMedGoogle Scholar
  103. Grandgenett, D. P., Gerard, G. F., and Green, M., 1972, Ribonuclease H: A ubiquitous activity in virions of ribonucleic acid tumor viruses, J. Virol. 10: 1136.PubMedGoogle Scholar
  104. Grandgenett, D. P., Gerard, G. F., and Green, M., 1973, A single subunitfrom avian myeloblastosis virus with both RNA-directed DNA polymerase and ribonuclease H activity (RNA virus/polyacrylamide gel electrophoresis), Proc. Natl. Acad. Sci. 70: 230.PubMedGoogle Scholar
  105. Green, M., 1970, Oncogenic viruses, Ann. Rev. Biochem. 39: 701.PubMedGoogle Scholar
  106. Green, M., 1970, Oncogenic viruses, Ann. Rev. Biochem. 39: 701.PubMedGoogle Scholar
  107. Hanafusa, H., 1965, Analysis of the defectiveness of Rous sarcoma virus. III. Determining influence of a new helper virus on the host range and susceptibility to interference of RSV, Virology 25: 248.PubMedGoogle Scholar
  108. Hanafusa, H., 1969, Rapid transformation of cells by Rous sarcoma virus, Proc. Natl. Acad. Sci. 63: 318.PubMedGoogle Scholar
  109. Hanafusa, H., and Hanafusa, T., 1971, Noninfectious RSV deficient in DNA polymerase, Virology 43: 313.PubMedGoogle Scholar
  110. Hanafusa, T., Hanafusa, H., and Miyamoto, T., 1970, Recovery of a new virus from apparently normal chick cells by infection with avian tumor viruses, Proc. Natl. Acad. Sci. 67: 1797.PubMedGoogle Scholar
  111. Hanafusa, T., Hanafusa, H., Miyamoto, T., and Fleissner, E., 1972a, Existence and expression of tumor virus genes in chick embryo cells, Virology 47: 475.Google Scholar
  112. Hanafusa, H., Baltimore, D., Smoler, D., Watson, K. F., Yaniv, A., and Spiegelman, S., 1972, Absence of polymerase protein in virions of alpha-type Rous sarcoma virus, Science 177:1188. Google Scholar
  113. Hartley, J. W., and Rowe, W. P., 1966, Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles, Proc. Natl. Acad. Sci. 55: 780.Google Scholar
  114. Hatanaka, M., and Hanafusa, H., 1970, Analysis of a functional change in membrane in the process of cell transformation by Rous sarcoma virus; alteration in the characteristics of sugar transport, Virology 41: 647.PubMedGoogle Scholar
  115. Hatanaka, M., Kakefuda, T., Gilden, R. V., and Callan, E. A. O:, 1971, Cytoplasmic DNA synthesis induced by RNA tumor viruses (mouse embryo fibroblasts/murine sarcoma virus/Rauscher murine leukemia virus/autoradiography), Proc. Natl. Acad. Sci. 68:1844.Google Scholar
  116. Hausen, P., and Stein, H., 1970, Ribonuclease H: An enzyme degrading the RNA moiety of RNA-DNA hybrids, Europ. J. Biochem. 14: 278.PubMedGoogle Scholar
  117. Hayward, W. S., and Hanafusa, H., 1973, Detection of avian tumor virus RNA in uninfected chicken embryo cells, J. Virol. 11: 157.PubMedGoogle Scholar
  118. Hehlmann, R., Kufe, D., and Spiegelman, S., 1972, RNA in human leukemic cells related to the RNA of a mouse leukemia virus (leukocytes/RNA-DNA hybridization/Rauscher virus/polysomal RNA), Proc. Natl. Acad. Sci. 69: 435.PubMedGoogle Scholar
  119. Hill, M., and Hillova, J., 1972a, Virus recovery in chicken cells tested with Rous sarcoma cell DNA, Nature New Biol. 237: 35.Google Scholar
  120. Hill, M., and Hillova, J., 19726, Recovery of the temperature-sensitive mutant of Rous sarcoma virus from chicken cells exposed to DNA extracted from hamster cells transformed by the mutant, Virology 49:309.Google Scholar
  121. Hobom-Schnegg, B., Robinson, H. L., and Robinson, W. S., 1970, Replication of Rous sarcoma virus in sychronized cells, J. Gen. Virol. 7: 85.Google Scholar
  122. Huang, A. S., Besmer, P., Chu, L., and Baltimore, D., 1973, Growth of pseudotypes of vesicular stomatitis virus with N-tropic murine leukemia virus coats in cells resistant to N-tropic viruses, J. Virol. 12: 659.PubMedGoogle Scholar
  123. Huebner, R. J., and Gilden, R. V., 1971, Inherited RNA viral genomes (virogenes and oncogenes) in the etiology of cancer, in: RNA Viruses and Host Genomes in Oncogenesis( P. Emmelot and P. Bentvelzen, eds.), pp. 197–219, North-Holland, Amsterdam, American Elsevier, New York.Google Scholar
  124. Huebner, R. J., and Todaro, G. J., 1969, Oncogenes of RNA tumor viruses as determinants of cancer, Proc. Natl. Acad. Sci. 64: 1087.PubMedGoogle Scholar
  125. Humphries, E. H., and Temin, H. M., 1972, Cell cycle-dependent activation of Rous sarcoma virus-infected stationary chicken cells: Avian leukosis virus group-specific antigens and ribonucleic acid, J. Virol. 10: 82.PubMedGoogle Scholar
  126. Hung, P. P., 1973, Rescue of chemically inactivated Rous sarcoma virus transforming activity by avian leukosis virus, Virology, 53: 463.PubMedGoogle Scholar
  127. Hurwitz, J., and Leis, J. P., 1972, RNA-dependent DNA polymerase activity of RNA tumor viruses. I. Directing influence of DNA in the reaction, J. Virol.9: 130.PubMedGoogle Scholar
  128. Ishizaki, R., Luftig, R. B., and Bolognesi, D. P., 1973, Outer membrane of avian myeloblastosis virus,/. Virol. 12: 1579.Google Scholar
  129. Jacquet, M., Groner, Y., Monroy, G., and Hurwitz, J., 1974, The in vitrosynthesis of avian myeloblastosis viral RNA sequences (RNA synthesis/avian myeloblastosis virus/chromatin), Proc. Natl. Acad. Sci. USA 71: 3145.Google Scholar
  130. Johnson, G. S., Friedman, R. M., and Pastan, I., 1971, Restoration of several morphological characteristics of normal fibroblasts in sarcoma cells treated with adenosine-3:5-cyclic monophosphate and its derivatives, Proc. Natl. Acad. Sci. 68: 425.Google Scholar
  131. Kacian, D. L., Watson, K. F., Burny, A., and Spiegelman, S., 1971, Purification of the DNA polymerase of avian myeblastosis virus, Biochim. Biophys. Acta 246: 365.PubMedGoogle Scholar
  132. Kang, C.-Y., and Temin, H. M., 1973, Lack of sequence homology among RNAs of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and chicken endogenous RNA-directed DNA polymerase activity, J. Virol. 12: 1314.PubMedGoogle Scholar
  133. kapeller, M., and Doljanski, F., 1972, Agglutination of normal and Rous sarcoma virus-transformed chick embryo cells by concanavalin A and wheat germ agglutinin, Nature New Biol. 235: 184.PubMedGoogle Scholar
  134. Kara, J., 1968, Induction of cellular DNA synthesis in chick embryo fibroblasts infected with Rous sarcoma virus in culture, Biochem. Biophys. Res. Commun. 32: 817.Google Scholar
  135. Karpas, A., and Milstein, C., 1973, Recovery of the genome of murine sarcoma virus (MSV) after infection of cells with nuclear DNA from MSV transformed non-virus producing cells, Europ. J. Cancer 9: 295.Google Scholar
  136. Kawai, S., and Hanafusa, H., 1971, The effects of reciprocal changes in temperature on the transformed state of cells infected with a Rous sarcoma virus mutant, Virology 46: 470.PubMedGoogle Scholar
  137. Kawai, S., and Hanafusa, H., 1972a, Plaque assay for some strains of avian leukosis virus, Virology 48: 126.Google Scholar
  138. Kawai, S., and Hanafusa, H., 19726, Genetic recombination with avian tumor virus, Virology 49:37.Google Scholar
  139. Kawai, S., and Hanafusa, H., 1973, Isolation of defective mutant of avian sarcoma virus, Proc. Natl. Acad. Sci. 70: 3493.PubMedGoogle Scholar
  140. 42.
    Keller, W., and Crouch, R., 1972, Degradation of DNA • RNA hybrids by ribonuclease H and DNA M BISHOP polymerases of cellular and viral origin, Proc. Natl. Acad. Sci. 69: 3360.PubMedGoogle Scholar
  141. Kotler, M., 1971, Interactions of avian sarcoma virus with rat embryo cells in cell culture, J. Gen. Google Scholar
  142. AND Virol. 12:197. Google Scholar
  143. H. E. Varmus Krontiris, T. G., Soeiro, R., and Fields, B. N., 1973, Host restriction of Friend leukemia virus: Role of the viral outer coat, Proc. Natl. Acad. Sci. 70: 2549.Google Scholar
  144. Kufe, D., Hehlmann, R., and Spiegelman, S., 1973, RNA related to that of a murine leukemia virus in Burkitt’s tumors and nasopharyngeal carcinomas, Proc. Natl. Acad. Sci. 70: 5.PubMedGoogle Scholar
  145. Kurth, R., and Bauer, H., 1972a, Cell-surface antigens induced by avian RNA tumor viruses: Detection by a cytotoxicity microassay, Virology 47: 426.Google Scholar
  146. Kurth, R., and Bauer, H., 19726, Common tumor specific antigens on cells of different species transformed by avian RNA tumor viruses, Virology 49:145.Google Scholar
  147. Kurth, R., and Bauer, H., 1973, Avian oncronavirus-induced tumor antigens of embryonic and unknown origin, Virology 56: 496.PubMedGoogle Scholar
  148. Lai, M. M. C., and Duesberg, P. H., 1972, Differences between the envelope glycoproteins and glycopeptides of avian tumor viruses released from transformed and nontransformed cells, Virology 50: 359.PubMedGoogle Scholar
  149. Lai, M. M.-C., Duesberg, P. H., Horst, J., and Vogt, P. K., 1973, Avian tumor virus RNA: A comparison of three sarcoma viruses and their transformation-defective derivatives by oligonucleotide fingerprinting and DNA-RNA hybridization, Proc. Natl. Acad. Sci. 70: 2266.PubMedGoogle Scholar
  150. Leis, J. P., and Hurwitz, J., 1972, RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction, J. Virol. 9: 130.PubMedGoogle Scholar
  151. Leis, J. P., Berkower, I., and Hurwitz, J., 1973, Mechanism of action of ribonuclease H isolated from avian myeloblastosis virus and Escherichia coli(RNA-dependent DNA polymerase/processive exonuclease/E. coliendonuclease), Proc. Natl. Acad. Sci. 70: 466.PubMedGoogle Scholar
  152. Leis, J. P., Berkower, I., and Hurwitz, J., 1973, Mechanism of action of ribonuclease H isolated from avian myeloblastosis virus and Escherichia coli(RNA-dependent DNA polymerase/processive exonuclease/E. coliendonuclease), Proc. Natl. Acad. Sci. 70: 466.PubMedGoogle Scholar
  153. Leong, J. A., Levinson, W., and Bishop, J. M., 1972a, Synchronization of Rous sarcoma virus production in chick embryo cells, Virology 47:133. Google Scholar
  154. Leong, J. A., Garapin, A. C., Jackson, N., Fanshier, L., Levinson, W., and Bishop, J. M., 19726, Virus specific ribonucleic acid in cells producing Rous sarcoma virus: Detection and characterization, J. Virol. 9:891.Google Scholar
  155. Levinson, W., Bishop, J. M., Quintrell, N., and Jackson, J., 1970, Presence of DNA in Rous sarcoma virus, Nature (Lond.) 227: 1023.Google Scholar
  156. Levinson, W., Heilbron, D., and Jackson, J., 1971, Behavior of chick embryo cells in tissue culture when infected with Rous sarcoma virus. I. Loss of contact inhibition, J. Natl. Cancer Inst. 46: 323.PubMedGoogle Scholar
  157. Levinson, W. E., Varmus, H. E., Garapin, A. C., and Bishop, J. M., 1972, DNA of Rous sarcoma virus: Its nature and significance, Science 175: 76.PubMedGoogle Scholar
  158. Lieber, M. M., Benveniste, R. E., Livingston, D. M., and Todaro, G. J., 1973, Mammalian cells in culture frequently release type C viruses, Science 182: 56.PubMedGoogle Scholar
  159. Linial, M., and Mason, W. S., 1973, Characterization of two conditional early mutants of Rous sarcoma virus, Virology 53: 258.PubMedGoogle Scholar
  160. Livingston, D. M., and Todaro, G. J., 1973, Endogenous type C virus from a cat cell clone with properties distinct from previously described feline type C virus, Virology 53: 142.PubMedGoogle Scholar
  161. Loeb, L. A., TaRtof, K. D., and Travaglini, E. C., 1973, Copying natural RNAs with E. coliDNA polymerase I, Nature New Biol. 242: 66.PubMedGoogle Scholar
  162. Love, D. N., and Weiss, R. A., 1974, Pseudotypes of vesicular stomatitis virus determined by exogenous and endogenous avian RNA tumor viruses, Virology 57: 271.PubMedGoogle Scholar
  163. Lovinger, G. G., Klein, R. A., Gilden, R. V., and Hatanaka, M., 1973, The effect of cordycepin on cell transformation by RNA tumor viruses, Virology 55: 524.PubMedGoogle Scholar
  164. Lowy, D. R., Rowe, W. P., Teich, N., and Hartley, J. W., 1971, Murine-leukemia virus: High-frequency activation in vitroby 5-iododeoxyuridine and 5-bromodeoxyuridine, Science 174: 155.PubMedGoogle Scholar
  165. Macieiro-Coelho, A., Hiu, I. J., and Garcia-Giralt, E., 1969, Stimulation of DNA synthesis in resting stage human fibroblasts after infection with Rous sarcoma virus, Nature (Lond.) 222: 1172.Google Scholar
  166. Macpherson, I. A., 1971, Reversion in cells transformed by tumour viruses, Proc. Roy. Soc. (Biol.) 177: 41.Google Scholar
  167. Maisel, J., Klement, V., Lai, M. M.-C., Ostertag, W., and Duesberg, P., 1973, Proc. Natl. Acad. Sci. 70: 3536.PubMedGoogle Scholar
  168. Markham, P. D., and Baluda, M. A., 1973, Integrated state of oncornavirus DNA in normal chicken cells and in cells transformed by avian myeloblastosis virus, J. Virol. 12: 721.PubMedGoogle Scholar
  169. Marshall, S., and Gillespie, D., 1972, Poly U tracts absent from viral RNA, Nature New Biol. 240: 43.PubMedGoogle Scholar
  170. Martin, G. S., 1970, Rous sarcoma virus: A function required for the maintenance of the transformed state, Nature (Lond.) 227: 1021.Google Scholar
  171. Martin, G. S., and Duesberg, P. H., 1972, The a subunit in the RNA of transforming avian tumor viruses. I. Occurrence in different virus strains. II. Spontaneous loss resulting in nontransforming variants, Virology 47: 494.PubMedGoogle Scholar
  172. Martin, G. S., Venuta, S., Weber, M., and Rubin, H., 1971, Temperature-dependent alterations in sugar transport in cells infected by a temperature-sensitive mutant of Rous sarcoma virus, Proc. Natl. Acad. Sci. 68: 2739.Google Scholar
  173. May, J. T., Somers, K. D., and Kit, S., 1972, Defective mouse sarcoma virus deficient in DNA polymerase activity, J. Gen. Virol. 16: 223.PubMedGoogle Scholar
  174. Mccaffrey, R., Smoler, D. F., and Baltimore, D., 1973, Terminal deoxynucleotidyl trasferase in a case of childhood acute lymphoblastic leukemia, Proc. Natl. Acad. Sci. 70: 521.PubMedGoogle Scholar
  175. Meier, H., Taylor, B. A., Cherry, M., and Huebner, R. J., 1973, Host-gene control of type C RNA tumor virus expression and tumorigenesis in inbred mice (reciprocal backcross progenies/ complement-fixing antigens/dominant genes/genetic markers), Proc. Natl. Acad. Sci. 70: 1450.PubMedGoogle Scholar
  176. Mizutani, S., and Temin, H. M., 1971, Enzymes and nucleotides in virions of Rous sarcoma virus, J. Virol. 8: 409.PubMedGoogle Scholar
  177. Modak, M. J., Marcus, S. L., and Cavalieri, L. F., 1973, DNA complementary to rabbit globin mRNA made by E. colipolymerase I, Biochem. Biophys. Res. Commun. 55: 1.PubMedGoogle Scholar
  178. Molling, K., Bolognesi, D. P., Bauer, H., Busen, W., Plassmann, H. W., and Hausen, P., 1971, Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids, Nature New Biol. 234: 240.PubMedGoogle Scholar
  179. Moore, E. G., and Temin, H. M., 1971, Lack of correlation between conversion by RNA tumour viruses and increased agglutinability of cells by concanavalin A and wheat germ agglutinin, Nature (Lond.) 231: 117.Google Scholar
  180. Muller, M., and Grossmann, H., 1972, An antigen in human breast cancer sera related to the murine mammary tumour virus, Nature New Biol. 237:116. Google Scholar
  181. Nandi, S., and Mcgrath, C. M., 1973, Mammary neoplasia in mice, Advan. Cancer Res. 17: 353.Google Scholar
  182. Neiman, P. E., 1972, Rous sarcoma virus nucleotide sequences in cellular DNA: Measurement by RNA-DNA hybridization, Science 178: 750.PubMedGoogle Scholar
  183. Nermut, M. V., Hermann, F., and Schafer, W., 1972, Poperties of mouse leukemia viruses. III. Electron microscopic appearance as revealed after conventional preparation techniques as well as freeze-drying and freeze-etching, Virology49: 345.Google Scholar
  184. Nichols, J. L., and Waddell, M., 1973, Comparison of free and 80S RNA-associated RNAs of mouse L cell virions, Nature New Biol. 243: 236.PubMedGoogle Scholar
  185. Nlcolson, G. L., 1971, Difference in topology of normal and tumour cell membranes shown by different surface distributions of ferritin-conjugated concanavalin A, Nature New Biol. 233: 244.Google Scholar
  186. Nomura, S., Fischinger, P. J., Mattern, C. F. T., Gerwin, B., and Dunn, K. J., 1973, Revertants of mouse cells transformed by murine sarcoma virus. II. Flat variants induced by fluorodeoxyuridine and colcemid, Virology 56: 152.PubMedGoogle Scholar
  187. Noonan, K. D., and Burger, M. M., 1973, Binding of 3H concanavalin A to normal and transformed cells, J. Biol. Chem. 248: 4286.PubMedGoogle Scholar
  188. Nowinski, R. C., Old, L. J., Sarkar, N. H., and Moore, D. H., 1970, Common properties of the oncogenic RNA viruses (oncornaviruses), Virology 42: 1152.PubMedGoogle Scholar
  189. Nowinski, R. C., Fleissner, E., Sarkar, N. H., and Aoki,T., 1972a, Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. II. Mammalian leukemia-sarcoma viruses, J. Virol.9: 359.Google Scholar
  190. Nowinski, R. C., Watson, K. F., Yaniv, A., and Spiegelman, S., 1972b, Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. II. Comparison of avian deoxyribonucleic acid polymerases, J. Virol. 10:959.Google Scholar
  191. Obara, T., Bolognesi, D. P., and Bauer, H., 1971, Ribosomal RNA in avian leukosis virus particles, Int. J. Cancer 7: 535.PubMedGoogle Scholar
  192. Oroszlan, S., Hatanaka, M., Gilden, R. V., and Huebner, R. J., 1971, Specific inhibition of mammalian ribonucleic acid C-type virus deoxyribonucleic acid polymerases by rat antisera, J. Virol. 8: 816.PubMedGoogle Scholar
  193. Ossowski, L., Quigley, J. P., Kellerman, G. M., and Reich, E., 1973, Fibrinolysis associated with oncogenic transformation: Requirement of plasminogen for correlated changes in cellular morphology, colony formation in agar, and cell migration, J. Exp. Med. 138: 1056.PubMedGoogle Scholar
  194. Otten, J., Johnson, G. S., and Pastan, I., 1971, Cyclic AMP levels in fibroblasts: Relationship to growth rate and contact inhibition of growth, Biochem. Biophys. Res. Commun. 44: 1192.PubMedGoogle Scholar
  195. Otten, J., Bader, J. P., Johnson, G. J., and Pastan, I., 1972, A mutation in a Rous sarcoma virus gene that controls adenosine (ZH,5-monophosphate levels and transformation, J. Biol. Chem. 247: 1632.PubMedGoogle Scholar
  196. Ozanne, B., and Sambrook, J., 1971, Binding of labelled Con A and wheat germ agglutinin to normal and transformed cells, Nature New 101. 232:156. Google Scholar
  197. Panem, S., and Kirsten, W. H., 1973, release of mouse leukemia-sarcoma virus from synchronized cells, J. Natl. Cancer Inst. 50:563.Google Scholar
  198. Paran, M., Gallo, R. C., Richardson, L. S., and Wu, A. M., 1973, Adrenal corticosteroids enhance production of C-type virus induced by 5-iodo-2-deoxyuridine from cultured mouse fibroblasts, Proc. Natl. Acad. Sci. 70: 2391.PubMedGoogle Scholar
  199. Parks, W. P., and Scolnick, E. M., 1972, Radioimmunoassay of mammalian type C viral proteins: Interspecies antigenic reactivities of the major internal polypeptide, Proc. Natl. Acad. Sci. 69: 1766.PubMedGoogle Scholar
  200. Parks, W. P., Scolnick, E. M., Ross, J., Tdaro, G. J., and Aaronson, S. A., 1972, Immunological relationships of reverse transcriptase from ribonucleic acid tumor viruses, J. Virol. 9: 110.PubMedGoogle Scholar
  201. Parsons, J. T., Coffin, J. M., Haroz, R. K., Bromley, P. A., and Weissman, C., 1973, Quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with Rous sarcoma virus, J. Virol. 11: 761.PubMedGoogle Scholar
  202. Parsons, J. T., Coffin, J. M., Haroz, R. K., Bromley, P. A., and Weissman, C., 1973, Quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with Rous sarcoma virus, J. Virol. 11: 761.PubMedGoogle Scholar
  203. Payne, L. N., and Chubb, R., 1968, Studies on the nature and genetic control of an antigen in normal chick embryos which reacts in the COFAL test, J. Gen. Virol. 3: 379.PubMedGoogle Scholar
  204. Peebles, P. T., Haapala, D. K., and Gazdar, A. F., 1972, Deficiency of viral ribonucleic acid-dependent deoxyribonucleic acid polymerase in noninfectious virus-like particles released from murine sarcoma virus-transformed hamster cells, J. Virol. 9: 488.PubMedGoogle Scholar
  205. Phillips, L. A., Hollis, V. W., Bassin, R. H., and Fischinger, P. J., 1973, Characterization of RNA from noninfectious virions produced by sarcoma positive-leukemia negative transformed 3T3 cells, Proc. Natl. Acad. Sci. 70: 3002.PubMedGoogle Scholar
  206. plralno, f., 1967, The mechanism of genetic resistance of chick embryo cells to infection by Rous sarcoma virus-Bryan strain, Virology32: 700.Google Scholar
  207. Poste, G., and Reeve, p., 1972, Agglutination of normal cells by plant lectins following infection with nononcogenic viruses, Nature New Biol. 237: 113.PubMedGoogle Scholar
  208. Proudfoot, N. J., and Brownlee, G. G., 1974, Nucleotide sequence adjacent to polyadenylic acid in globin messenger RNA, FEBS Letters 38: 179.Google Scholar
  209. Quintrell, N., Varmus, H. E., Bishop, J. M., Nicolson, M. O., and Mcallister, R. M., 1974, Homologies among the nucleotide sequences of the genomes of C-type viruses, Virology 58: 568.PubMedGoogle Scholar
  210. Randerath, K., Rosenthal, L. J., and Zamecnik, P. C., 1971, Base composition differences between avian myeloblastosis virus transfer RNA, and transfer RNA isolated from host cells, Proc. Natl. Acad. Sci. 68: 3233.PubMedGoogle Scholar
  211. Reich, E., 1973, Tumor-associated fibrinolysis, Fed. Proc. 32: 2174.PubMedGoogle Scholar
  212. Richert, N. J., and Hare, J. D., 1972, Distinctive effects of inhibitors of mitochondrial function on Rous sarcoma virus replication and malignant transformation, Biochem. Biophys. Res. Commun. 46:5. Google Scholar
  213. Rifkin, D. B. and Compans, R. W., 1971, Identification of the spike proteins of Rous sarcoma virus, Virology 46: 485.PubMedGoogle Scholar
  214. Robinson, W. S., Hung, P., Robinson, H. L., and Ralph, D. D., 1970, Proteins of avian tumor viruses with different coat antigens, J. Virol. 6: 695.PubMedGoogle Scholar
  215. Romano, A. H., and Colby, C., 1973, SV40 virus transformation of mouse 3T3 cells does not specifically enhance sugar transport, Science 179: 1238.PubMedGoogle Scholar
  216. Rosenthal, L. J., and Zamecnik, P. C., 1973a, Minor base composition of “70-associated” 4S RNA from avian myeloblastosis virus (tumor virus/isotope derivative method/titanium fluorography), Proc. Natl. Acad. Sci. 70: 727.Google Scholar
  217. Rosenthal, L. J., and Zamecnik, P. C., 19736, Amino-acid acceptor activity of the “70S-associated” 4S RNA from avian myeloblastosis virus (oncogenic RNA virus/RNA bound to 70S RNA), Proc. Natl. Acad. Sci. 70:1184.Google Scholar
  218. Rosenthal, P. N., Robinson, H. L., Robinson, W. S., Hanafusa, T., and Hanafusa, H., 1971, DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA, Proc. Natl. Acad. Sci. 68: 2336.PubMedGoogle Scholar
  219. Rous, P., 1911, A sarcoma of the fowl transmissible by an agent separable from the tumor cells, J. Exp. Med. 13:397.Google Scholar
  220. Rowe, W. P., 1971, The kinetics of rescue of the murine sarcoma virus genome from a nonproducer line of transformed mouse cells, Virology 46: 369.PubMedGoogle Scholar
  221. Rowe, W. P., Hartley, J. W., Lander, M. R., Pugh, W. E., and Teich, N., 1971, Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus, Virology 46: 866.PubMedGoogle Scholar
  222. Rowe, W. P., Hartley, J. W., and Bremner, T., 1972, Genetic mapping of a murine leukemia virus-inducing locus of AKR mice, Science 178: 860.PubMedGoogle Scholar
  223. Ruprecht, R. M., Goodman, N. C., and Spiegelman, S., 1973, Determination of natural host taxonomy of RNA tumor viruses by molecular hybridization: Application to RD-114, a candidate human virus, Proc. Natl. Acad. Sci. 70: 1437.PubMedGoogle Scholar
  224. Rymo, L., Parsons, J. T., Coffin, J. M., and Weissmann, C., 1974, In vitrosynthesis of Rous sarcoma virus-specific RNA is catalyzed by a DNA-dependent RNA polymerase (actinomycin D/a-amanitin/assay of virus-specific RNA), Proc. Natl. Acad. Sci. USA 71: 2782.Google Scholar
  225. Sarin, P. S., and Gallo, R. C., 1973, RNA directed DNA polymerase, in: International Review of Science Series in Biochemistry, Vol. 6 (K. Burton, ed.), Chap. 8, Butterworth, Oxford.Google Scholar
  226. Sarkar, N. H., Moore, D. H., and Nowinski, R. C., 1971a, Symmetry of the nucleocapsid of the oncornaviruses, in: RNA Viruses and Host Genome in Oncogenesis, ( P. Emmelot and P. Bentvelzen, eds.), pp. 71–79, North-Holland, Amsterdam, American Elsevier, New York.Google Scholar
  227. Sarkar, N. H., Nowinski, R. C., and Moore, D. H., 19716, Helical nucleocapsid structure of the oncogenic ribonucleic acid viruses (oncornaviruses), J. Virol. 8:564.Google Scholar
  228. Sawyer, R. C., and Dahlberg, J. E., 1973, Small RNAs of Rous sarcoma virus: Characterization by two-dimensional polyacrylamide gel electrophoresis and fingerprint analysis, J. Virol. 12: 1226.PubMedGoogle Scholar
  229. Sawyer, R. C., Harada, F., and Dahlberg, J. E., 1974, An RNA primer for Rous sarcoma virus DNA synthesis: Isolation from uninfected host cells. J. Virol 13: 1302.PubMedGoogle Scholar
  230. Schafer, W., Pister, L., Hunsmann, G., and Moennig, V., 1973, Comparative serological studies on type C viruses of various mammals, Nature New Biol. 245: 75.PubMedGoogle Scholar
  231. Scheele, C. M., and Hanafusa, H., 1971, Proteins of helper-dependent RSV, Virology 45: 401.PubMedGoogle Scholar
  232. Schincariol, A. L., and Joklik, W. E., 1973, Early synthesis of virus-specific RNA and DNA in cells rapidly transformed with Rous sarcoma virus, Virology 56: 532.PubMedGoogle Scholar
  233. Scolnick, E. M., Parks, W. P., and Todaro, G. J., 1972a, Reverse transcriptases of primate viruses as immunological markers, Science 177: 1119.Google Scholar
  234. Scolnick, E. M., Stephensen, J. R., and Aaronson, S. A., 19726, Isolation of temperature sensitive mutants of murine sarcoma virus, J. Virol. 10:653.Google Scholar
  235. Scolnick, E. M., Parks, W. P., Todaro, G. J., and Aaronson, S. A., 1972c, Immunological characterization of primate C-type virus reverse transcriptases, Nature New Biol. 235: 35.Google Scholar
  236. Scolnick, E. M., Rands, E., Williams, D., and Parks, W. P., 1973, Studies on the nucleic acid sequences of Kirsten sarcoma virus: A model for formation of a mammalian RNA-containing sarcoma virus, J. Virol. 12: 458.PubMedGoogle Scholar
  237. Scolnick, E. M., Parks, W., Kawakami, T., Kohne, D., Okale, H., Gilden, R., and Hatanaka, M., 1974, Primates and murine type-C viral nucleic acid association kinetics: Analysis of model systems and natural tissues, J. Virol. 13: 363.PubMedGoogle Scholar
  238. Sharon, N., and Lis, H., 1972, Lectins: Cell-agglutinating and sugar-specific proteins, Science 177: 949.PubMedGoogle Scholar
  239. Siegert, W., Konings, R. N. J., Bauer, H., and Hofschneider, P. H., 1972, Translation of avian myeloblastosis virus RNA in a cell-free lysate of Escherichia coli, Proc. Natl. Acad. Sci. 69: 888.PubMedGoogle Scholar
  240. Silber, R., Malathi, V. G., Schulman, L. H., Hurwitz, J., and Duesberg, P., 1973, Studies of the Rous sarcoma virus RNA: Characterization of the 5-terminus, Biochem. Biophys. Res. Commun. 50:467.Google Scholar
  241. Smith, g. h., and Wlvel, n. a., 1973, Intracytoplasmic aparticles: Mouse mammary tumor virus nucleoprotein cores? J. Virol. 11: 575.PubMedGoogle Scholar
  242. Smith. H. S., Gelb, L. D., and Martin, M. A., 1972, Detection and quantitation of simian virus 40 genetic material in abortively transformed BALB/3T3 clones, Proc. Natl. Acad. Sci. 69: 152.PubMedGoogle Scholar
  243. Smoler, D., Molineux, I., and Baltimore, D., 1971, Direction of polymerization by the avian myeloblastosis virus deoxyribonucleic acid polymerase, J. Biol. Chem. 246: 7697.PubMedGoogle Scholar
  244. Somers, K. D., May, J. T., and Kit, S., 1973, Control of gene expression in rat cells transformed by a cold-sensitive murine sarcoma virus (MSV) mutant, Intervirology 1: 176.PubMedGoogle Scholar
  245. Somers, K. D., May, J. T., and Kit, S., 1973, Control of gene expression in rat cells transformed by a cold-sensitive murine sarcoma virus (MSV) mutant, Intervirology 1: 176.PubMedGoogle Scholar
  246. Stephenson, J. R., and Aaronson, S. A., 1971, Murine sarcoma and leukemic viruses: Genetic differences determined by RNA-DNA hybridization, Virology 46: 480.PubMedGoogle Scholar
  247. Stephenson, J. R., and Aaronson, S. A., 1973, Segregation of loci for C-type virus induction in strains of mice with high and low incidence of leukemia, Science 180: 865.PubMedGoogle Scholar
  248. Stephenson, J. R., Scolnick, E. M., and Aaronson, S. A., 1972, Genetic stability of the sarcoma viruses in murine and avian sarcoma virus transformed nonproducer cells, Int. J. Cancer 9: 577.PubMedGoogle Scholar
  249. Stephenson, J. R., Reynolds, R. K., and Aaronson, S. A., 1973, Characterization of morphologic revertants of murine and avian sarcoma virus-transformed cells, J. Virol. 11: 218.PubMedGoogle Scholar
  250. Stephenson, J. R., Greenberger, J. S., and Aaronson, S. A., 1974, Oncogenicity of an endogenous C-type virus chemically activated from mouse cells in culture, J. Virol. 13: 237.PubMedGoogle Scholar
  251. Stephenson, M. L., Scott, J. F., and Zamecnik, P. C., 1973, Evidence that polyadenylic acid segment of “35S” RNA of avian myeloblastosis virus is located at the 3 OH terminus, Biochem. Biophys. Res. Commun. 55: 8.PubMedGoogle Scholar
  252. Strand, M., and August, J. T., 1974, Structural proteins of mammalian oncogenic RNA viruses: Multiple antigenic determinants of the major internal protein and envelope glycoprotein, J. Virol. 13: 171.PubMedGoogle Scholar
  253. Svoboda, J., 1972, The biology of avian tumor viruses and the role of host cell in the modification of avian tumor virus expression, in: RNA Viruses and Host Genome in Oncogenesis( P. Emmelot and P. Bentvelzen, eds.), pp. 81–92, North-Holland, Amsterdam, American Elsevier, New York.Google Scholar
  254. Svoboda, J., and Dourmashkin, R., 1969, Rescue of RSV from virogenic mammalian cells associated with chicken cells and treated with Sendai virus, J. Gen. Virol. 4: 523.PubMedGoogle Scholar
  255. Tanaka, H., Tamura, A., and Tsujimura, D., 1972, Properties of the intracytoplasmic A particles purified from mouse tumors, Virology 49: 61.PubMedGoogle Scholar
  256. Taylor, B. A., Neiher, H., and Myers, D. D., 1971, Host-gene control of C-type RNA tumor virus: Inheritance of the group-specific antigen of murine leukemia virus, Proc. Natl. Acad. Sci.63: 3190.Google Scholar
  257. Taylor, B. A., Meier, H., and Huebner, R. J., 1973, Genetic control of the group-specific antigen of murine leukemia virus, Nature New Biol. 241: 184.PubMedGoogle Scholar
  258. Taylor, J. M., Varmus, H. E., Faras, A. J., Levinson, W. E., and Bishop, J. M., 1974, Evidence for non-repetitive subunits in the genome of Rous sarcoma virus, J. Mol. Biol., 84: 217.PubMedGoogle Scholar
  259. Teich, N., Lowy, D. R., Hartley, J. W., and Rowe, W. P., 1973, Studies of the mechanism of induction of infectious murine leukemia virus from AKR mouse embryo cell lines by 5-iododeoxyuridine and 5-bromodeoxyuridine, Virology Virology 51: 163.Google Scholar
  260. Temin, H. M., 1961, Mixed infections with two types of Rous sarcoma virus, Virology 13: 158.PubMedGoogle Scholar
  261. Temin, H. M., 1963, The effects of actinomycin D on growth of Rous sarcoma virus in vitro, Virology 20: 577.Google Scholar
  262. Temin, H. M., 1965, The mechanism of carcinogenesis by avian sarcoma viruses. I. Cell multiplication and differentiation, J. Natl. Cancer Inst. 35: 679.PubMedGoogle Scholar
  263. Temin, H. M., 1967, Studies on carcinogenesis by avian sarcoma viruses, V. Requirement for new DNA synthesis and for cell division, J. Cell. Physiol. 69: 53.Google Scholar
  264. Temin, H. M., 1968, Carcinogenesis by avian sarcoma viruses, Cancer Res. 28: 1835.Google Scholar
  265. Temin, H. M., 1970, Formation and activation of the provirusof RNA sarcoma viruses, in: The Biology of Large RNA Viruses( R. D. Barry and B. W. J. Mahy, eds.), pp. 233–249, Academic Press, New York and London.Google Scholar
  266. Temin, H. M., 1971, Mechanism of cell transformation by RNA tumor viruses, Ann. Rev. Microbiol.25: 609.Google Scholar
  267. Temin, H. M., 1972, The RNA tumor viruses—Background and foreground, Proc. Natl. Acad. Sci. 69: 1016.PubMedGoogle Scholar
  268. Temin, H. M., and Baltimore, D., 1972, RNA-directed DNA synthesis and RNA tumor viruses, Advan. Virus Res. 17: 129.Google Scholar
  269. Temin, H. M., and Mizutani, S., 1970, RNA-dependent DNA polymerase in virions of Rous sarcoma virus, Nature (Lond.) 226: 1211.Google Scholar
  270. Ting, C.-C., Lavrin, D. H., Shiu, G., and Harberman, R. B., 1972, Expression of fetal antigens in tumor cells, Proc. Natl. Acad. Sci. 69: 1664.PubMedGoogle Scholar
  271. Todaro, G. J., 1972, “Spontaneous” release from clonal lines of “spontaneously” transformed BALB/3T3 cells, Nature New Biol. 240:157.Google Scholar
  272. Todaro, G. J., and Gallo, R. C., 1973, Immunological relatedness between a DNA polymerase from human acute leukemia cells and primate and mouse leukemia virus reverse transcriptase, Nature (Lond.) 244: 206.Google Scholar
  273. Todaro, G. J., and Huebner, R. J., 1972, The viral oncogene hypothesis: New evidence, Proc. Natl. Acad. Sci. 69: 1009.PubMedGoogle Scholar
  274. Travnicek, M., and Riman, J., 1970, Chromatographic differences between lysyl-tRNA’s from avian tumor virus BAI strain A and virus transformed cells, Biochim. Biophys. Acta199: 283.PubMedGoogle Scholar
  275. Tronick, S. R., Scolnick, E. M., and Parks, W. P., 1972, Reversible inactivation of the deoxyribonucleic acid polymerase of Rauscher leukemia virus, J. Virol. 10: 885.PubMedGoogle Scholar
  276. Tsuchida, M., Ribin, M. S., and Green, M., 1972, Viral RNA subunits in cells transformed by RNA tumor viruses, Science 176:1418. Google Scholar
  277. Twardzik, D., Simonds, J., Oskarsson, M., and Portugal, F., 1973, Translation of AKR-murine leukemia viral RNA in an E. colicell-free system, Biochem. Biophys. Res. Commun. 52: 1108.PubMedGoogle Scholar
  278. Varmus, H. E., Levinson, W. E., and Bishop, J. M., 1971, Extent of transcription by the RNA- dependent DNA polymerase of Rous sarcoma virus (RSV), Nature New Biol. 233: 19.PubMedGoogle Scholar
  279. Varmus, H. E., Bishop, J. M., Nowinski, R., and Sarkar, N., 1972a, Mammary tumor virus specific nucleotide sequences in DNA of high and low incidence mouse strains, Nature New Biol. 238: 189.Google Scholar
  280. Varmus, H. E., Weiss, R. A., Friis, R. R., Levinson, W., and Bishop, J. M., 19726, Detection of avian tumour virus-specific nucleotide sequences in avian cell DNAs, Proc. Natl. Acad. Sci. 69:20.Google Scholar
  281. Varmus, H. E., Bishop, J. M., and Vogt, P. K., 1973a, Synthesis and integration of Rous sarcoma virus-specific DNA in permissive and non-permissive hosts, in: Virus Research(Proceedings of the Second ICN-UCLA Symposium on Molecular Biology, C. F. Fox and W. S. Robinson, eds.), pp. 373–383, Academic Press, New York and London.Google Scholar
  282. Varmus, H. E., Bishop, J. M., and Vogt, P. K., 19736, Appearance of virus-specific DNA in mammalian cells following transformation by Rous sarcoma virus, J. Mol. Biol. 74:613.Google Scholar
  283. Varmus, H. E., Vogt, P. K., and Bishop, J. M., 1973c, Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts, Proc. Natl. Acad. Sci. 70: 3067.Google Scholar
  284. Varmus, H. E., Hansen, C. B., Medeiros, E., Deng, C. T., and Bishop, J. M., 1973d, Detection and characterization of RNA tumor virus-specific nucleotide sequences in cell DNA, in: Possible Episomes in Eukaryotes( Fourth Lepetit Colloquium, L. G. Silvestri, ed.), pp. 50–60, North-Holland, Amsterdam, American Elsevier, New York.Google Scholar
  285. Varmus, H. E., Quintrell, N., Medeiros, E., Bishop, J. M., and Nowinski, R. C., 1973e, Transcription of mouse mammary tumor virus genes in tissues from high and low tumor incidence mouse strains, J. Mol. Biol. 79: 663.Google Scholar
  286. Varmus, H. E., Quintrell, N., Medeiros, E., Bishop, J. M., and Nowinski, R. C., 1973e, Transcription of mouse mammary tumor virus genes in tissues from high and low tumor incidence mouse strains, J. Mol. Biol. 79: 663.Google Scholar
  287. Varmus, H. E., Guntaka, R. V., Fan, W. J. W., Heasley, S., and Bishop, J. M., 19746, Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus, Proc. Natl. Acad. Sci. USA 71:3874.Google Scholar
  288. Varmus, H. E., Heasley, S., and Bishop, J. M., 1974c, Use of DNA-DNA annealing to detect new virus-specific DNA sequences in chicken embryo fibroblasts after infection by avian sarcoma virus, J. Virol. 14: 895.Google Scholar
  289. Venuta, S., and Rubin, H., 1973, Sugar transport in normal and Rous sarcoma virus-transformed chick-embryo fibroblasts (3-O-methylglucose/glycose competition/efflux/facilitated diffusion/Km, V/max), Proc. Natl. Acad. Sci 70: 653.PubMedGoogle Scholar
  290. Verma, I. M., Meuth, N. L., Bromfeld, E., Manly, K. F., and Baltimore, D., 197l,Covalently linked RNA, DNA molecule as initial product of RNA tumour virus DNA polymerase, Nature New Biol. 233:131.Google Scholar
  291. Verma, I. M., Meuth, N. L., Fan, H., and Baltimore, D., 1974, Hamster leukemia virus DNA polymerase: Unique structure and lack of demonstrable endogenous activity, J. Virol., 13: 1075.PubMedGoogle Scholar
  292. Vogt, P. K., 1971a, Spontaneous segregation of nontransforming viruses from cloned sarcoma viruses, Virology 46: 939.Google Scholar
  293. Vogt, P. K., 19716, Genetically stable reassortment of markers during mixed infection with avian tumor viruses, Virology 46:947.Google Scholar
  294. Vogt, P. K., 1973, The genome of avian RNA tumor viruses: A discussion of four models, in: Possible Episomes in Eukaryotes(Proceedings of the Fourth Lepetit Colloquium, L. Silvestri, ed.), pp. 35–41, North-Holland, Amsterdam.Google Scholar
  295. Vogt, P. K., and Duesberg, P. H., 1973, On the mechanism of recombination between avian RNA tumor viruses, in: Virus Research(Proceedings of the Second ICN-UCLA Symposium on Molecular Biology, C. F. Fox and W. S. Robinson, eds.), pp. 505–512, Academic Press, New York and London.Google Scholar
  296. Vogt, P. K., and Friis, R. R., 1971, An avian leukosis virus related to RSV(O): Properties and evidence for helper activity, Virology 43: 223.PubMedGoogle Scholar
  297. Vogt, V. M., and Eisenman, R., 1973, Identification of a large polypeptide precursor of avian oncornavirus proteins, Proc. Natl. Acad. Sci. 70: 1734.PubMedGoogle Scholar
  298. Walker, T. A., Pace, N. R., Erikson, R. L., Erikson, E., and Behr, F., 1974, The 7S RNA common to oncornaviruses and normal cells is associated with polyribosomes, Proc. Natl. Acad. Sci. USA 71: 3390.PubMedGoogle Scholar
  299. Wang, L.-H., and Duesberg, P. H., 1973, DNA polymerase of murine sarcoma-leukemia virus: Lack of detectable RNase H and low activity with viral RNA and natural DNA templates, J. Virol. 12: 1512.PubMedGoogle Scholar
  300. Wanng, S. Koethari, R. M., and Taylor, M., 1973, Transfer RNA activities of Rous sarcoma and Rous associated viruses, Nature New Biol. 242:133.Google Scholar
  301. Warren, L., Critchley, D., and Macpherson, I., 1972, Surface glycoproteins and glycolipids of chicken embryo cells transformed by temperature-sensitive mutant of Rous sarcoma virus, Nature (Lond.) 235: 275.Google Scholar
  302. Weinberg, R., 1973, Nuclear RNA metabolism, Ann. Rev. Biochem. 42: 329.PubMedGoogle Scholar
  303. Weiss, R. A., 1971, Cell transformation induced by Rous sarcoma virus: Analysis of density dependence, Virology 46: 209.PubMedGoogle Scholar
  304. Weiss, R. A., and Payne, L. N., 1971, The heritable nature of the factor in chicken cells which acts as a helper virus for Rous sarcoma virus, Virology 45: 508.PubMedGoogle Scholar
  305. Weiss, R. A., Friis, R. R., Katz, E., and Vogt, P. K., 1971, Induction of avian tumor viruses in normal cells by physical and chemical carcinogens, Virology 46: 920.PubMedGoogle Scholar
  306. Weiss, R. A., Mason, W. S., and Vogt, P. K., 1973, Genetic recombinants and heterozygotes derived from endogenous and exogenous avian RNA tumor viruses, Virology 52: 535.PubMedGoogle Scholar
  307. Weissbach, A., Bolden, A., Muller, R., Hanafusa, H., and Hanafusa, T., 1972, Deoxyribonucleic acid polymerase activities in normal and leukovirus-infected chicken embryo cells, J, Virol. 10: 321.Google Scholar
  308. Witter, R., Frank, H., Moenning, V., Hunsmann, G., Lange, J., and Schafer, W., 1973, Properties of mouse leukemia viruses. IV. Hemagglutination assay and characterization of hemagglutinating surface components, Virology 54: 330.PubMedGoogle Scholar
  309. Wivel, N. A., and Smith, G. H., 1971, Distribution of intracisternal A particles in a variety of normal and neoplastic mouse tissues, Int. J. Cancer 7: 167.PubMedGoogle Scholar
  310. Wivel, N. A., Lenders, K. K., and Kuff, E. L., 1973, Structural organization of murine intracisternal A particles,/. Virol. 11: 329.Google Scholar
  311. Wollmann, R. L., and Kirsten, W. H., 1968, Cellular origin of a mouse leukemia viral RNA,/. Virol. 2: 1241.Google Scholar
  312. Wu, A. M., Ting, R. C., Paran, M., and Gallo, R. C., 1972, Cordycepin inhibits induction of murine leukovirus production by 5-iodo-2’-deoxyuridine, Proc. Natl. Acad. Sci. 69: 3820.PubMedGoogle Scholar
  313. Wyke, J. A., 1973, Complementation of transforming functions by temperature-sensitive mutants of avian sarcoma virus, Virology 54: 28.PubMedGoogle Scholar
  314. Wyke, J. A., and Linial, M., 1973, Temperature-sensitive avian sarcoma viruses: A physiological comparison of twenty mutants, Virology 53: 152.PubMedGoogle Scholar
  315. Yoshii, S., and Vogt, P. K., 1970, A mutant of Rous sarcoma virus (type 0) causing fusiform cell transformation, Proc. Soc. Exp. Biol. Med. 135: 297.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • J. Michael Bishop
    • 1
  • Harold E. Varmus
    • 1
  1. 1.Department of MicrobiologyUniversity of CaliforniaSan FranciscoUSA

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