Bacteriophages That Contain Lipid
The several types of lipid-containing bacteriophages that have been described so far do not constitute a natural grouping. They differ from each other in many ways including host range, nucleic acid type, mode of attachment to host cells, and location of the lipid in the virion. It is, however, useful to discuss them together since they are likely to prove of immense value in the elucidation of lipid-protein interactions and the biogenesis of structures containing lipid. The anticipated advantages of these viruses for the study of “membrane biology” are to be found in their rather simple and probably stoichiometric composition with respect to proteins and nucleic acids, which is characteristic of almost all viruses, as well as the ease of preparing large amounts of virus material for structural work and the possibility of isolating conditional-lethal mutants that will facilitate the investigation of the morphogenetic pathways employed by these viruses.
KeywordsNonsense Mutant Buoyant Density Early Protein Mature Virion Glycine Ethyl Ester
Unable to display preview. Download preview PDF.
- Cadden, S. P., and Sands, J. A., 1976, Proteins of a lipid containing bacteriophage which replicates in Escherichia coli: Phage PR4, Abst. Annu. Meet. Am. Soc. Microbiol., S20, p. 207.Google Scholar
- Diedrich, D. L., and Cota-Robles, E. H., 1974, Heterogeneity in lipid composition of the outer membrane and cytoplasmic membrane of Pseudomonas BAL-31, J. Bacte-riol. 119:1006–1018.Google Scholar
- Fiers, W., 1975, Chemical structure and biological activity of bacteriophage MS2 RNA, in: RNA Phages (N. D. Zinder, ed.), pp. 353–396, Cold Spring Harbor Laboratory, Cold Spring Harbor.Google Scholar
- Franklin, R. M., 1974, Structure and synthesis of bacteriophage PM2, with particular emphasis on the viral lipid bilayer, Curr. Top. Microbiol. Immunol. 68:108–159.Google Scholar
- Franklin, R. M., 1976, PM2 bacteriophage as a model for the structure and synthesis of lipid membranes, Cell Surface Rev. (in press).Google Scholar
- Gonzalez, C. F., Langenberg, W. G., Van Etten, J. L., and Vidaver, A. K., 1976, Ultrastructure of bacteriophage ϕ6: Arrangement of dsRNA and lipid envelope, Abstr. Annu. Proc. Am. Phytopathol. Soc. Abstr., No. 223.Google Scholar
- Harrison, S. C., Caspar, D. L. D., Camerini-Otero, R. D., and Franklin, R. M., 1971, Lipid and protein arrangement in bacteriophage PM2, Nature (London) New Biol. 229:197–201.Google Scholar
- Osborne, M. J., Gander, J. E., Parisi, E., and Carson, J., 1972, Mechanism of assembly of the outer membrane of Salmonella typhimurium: Isolation and characterization of cytoplasmic and outer membrane, J. Biol. Chem. 247:3962–3972.Google Scholar
- Schäfer, R., Huber, U., Franklin, R. M., and Seelig, J., 1975, Structure and synthesis of a lipid-containing bacteriophage. XXI. Chemical modifications of bacteriophage PM2 and the resulting alterations in acyl-chain motion in the PM2 membrane, Eur. J. Biochem. 58:291–296.PubMedCrossRefGoogle Scholar
- Spencer, R., 1963, Bacterial viruses in the sea, Symposium on Marine Microbiology (C. H. Oppenheimer, ed.), pp. 350–365, Thomas, Springfield, 111.Google Scholar
- Tsukagoshi, N., Petersen, M. H., Huber, U., Franklin, R. M., and Seelig, J., 1976b, Phase transitions in the membrane of a marine bacterium, Pseudomonas BAL-31, Eur. J. Biochem. 62:257–262.Google Scholar
- Weiner, A. M., and Weber, K., 1971, Natural read-through at the UGA termination signal of Qβ coat protein cistron, Nature (London) New Biol. 234:206–209.Google Scholar