The Bacterial Phosphoenolpyruvate Phosphotransferase System
Active transport. Systems of this type accumulate the solute in an unaltered form in the cytoplasm. The energy for the translocation is primarily derived from an energized membrane state (membrane potential or proton-motive force derived from electron transport or ATP hydrolysis).
Group translocation. This differs thermodynamically from “active transport” since the solute is accumulated in the cytoplasm in a derivatized form. Group translocation has so far been associated only with the translocation of sugars; these are accumulated in the form of phosphate esters. The mechanism responsible for this form of translocation is the phosphotransferase system (Figure 2) which uses phosphoenolpyruvate as its primary source of energy.
KeywordsClostridium Thermocellum Phosphotransferase System Phosphoryl Transfer Lactose Transport Phosphoryl Moiety
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- Burd, G. I., Andreeva, I. V., Shabolenko, V. P., and Gershanovich, V. N., 1968, Absence of phosphotransferase system components in mutant Escherichia coli K12 with a disrupted carbohydrate transfer system, Mol. Biol. 2:89.Google Scholar
- Burd, G. I., Boil’shakova, T. N., and Gershanovich, V. N., 1973, Relationship between β-galac-toside transport and phosphoenolpyruvate dependent phosphotransferase system in Escherichia coli K12, Mol. Biol. 7:318.Google Scholar
- Cirillo, V. P., and Razin, S., 1972, Distribution of phosphoenolpyruvate-dependent sugar phosphotransferase systems in Mycoplasma, J. Bacteriol. 113:212.Google Scholar
- Cordaro, J. C., Postma, P. W., and Roseman, S., 1974b, A mutation affecting membrane-bound enzymes in Salmonella typhimurium, Fed. Proc. 33:1326.Google Scholar
- Epstein, W., Jewett, S., and Fox, C. F., 1970, Isolation and mapping of phosphotransferase mutants in Escherichia coli, J. Bacteriol. 104:293.Google Scholar
- Gershanovich, V. N., Yurotskaya, N. V., and Burd, G. I., 1970, Pleiotropic disturbances of enzyme systems in Escherichia coli mutants with defects in Roseman’s phosphotransferase system, Mol. Biol. 4:534.Google Scholar
- Kabagk, H. R., 1968, The role of the phosphoenolpyruvate phosphotransferase system in the transport of sugars by isolated membrane preparation of Escherichia coli, J. Biol. Chem. 243:3711.Google Scholar
- Kundig, W., 1974b, The bacterial phosphoenolpyruvate phosphotransferase system: Molecular interactions and biological function, Proceedings 1st Inter sectional Congress of the International Societies for Microbiology, Tokyo, Japan, 1974, vol. 1, p. 613.Google Scholar
- Lehninger, A. L., 1971, Biochemistry, Worth Publishers, Inc., New York.Google Scholar
- Patni, N. J., and Alexander, J. K., 1971b, Catabolism of fructose and mannitol in Clostridium thermocellum: Presence of phosphoenolpyruvate: fructose phosphotransferase, fructose-1-phosphate kinase, phosphoenolpyruvate: mannitol phosphotransferase and mannitol 1-phosphate dehydrogenase in cell extracts, J. Bacteriol. 105:276.Google Scholar
- Roseman, S., 1972, Carbohydrate transport in bacterial cells, in: Metabolic Pathways, Vol. VI, Metabolic Transport (L. E. Hokin, ed.), p. 41, Academic Press, New York.Google Scholar
- Saier, M. H., Jr., and Roseman, S., 1971, Regulation of enzyme induction by a bacterial phosphotransferase system, Fed. Proc. 30:1097.Google Scholar
- Schaghter, H., 1973, On the interaction of Michaelis constants for transport, J. Biol. Chem. 248:974.Google Scholar
- Suzuki, F., Fukunishi, K., and Takeda, Y., 1969, Studies on ATP citrate lyase of rat liver. V. The binding site of phosphate, J. Biochem (Tokyo) 66:767.Google Scholar
- Weigel, N., and Powers, D. A., 1975, Studies on the primary structure of a phosphocarrier protein of the bacterial phosphotransferase system, Fed. Proc. 34:491.Google Scholar
- White, R. J., 1969, The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-glucosamine in Escherichia coli, Biochem. J. 118:89.Google Scholar