Membrane-Bound Enzymes in Plant Lipid Metabolism
Because of the heterogeneity of plant cells in terms of structure and function, assignment of membranous enzymes to a specific membrane system, i.e., plasma, organelle, or cell wall, is difficult. Progress in examining plant membrane-bound enzymes in detail has therefore been slow. Recently, however, density-gradient separation techniques, coupled with reliable marker enzymes, has been applied to a number of problems with some success (Moore et al., 1973). A further refinement is the employment of plant cells grown in suspension cultures under controlled conditions (Moore and Beevers, 1974). This material is reasonably uniform in age and development and has the added advantage of allowing the experimenter the opportunity of manipulating the milieu of the cell suspension under controlled conditions. With this background in mind we shall limit the contents of this chapter to a discussion of soluble and membrane-bound enzymes which participate in plant lipid metabolism.
KeywordsCastor Bean Ricinoleic Acid Acyl Carrier Protein Spinach Chloroplast Fatty Acid Synthetase
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