Membrane-Bound Enzymes in Plant Lipid Metabolism

  • P. K. Stumpf


Because of the heterogeneity of plant cells in terms of structure and function, assignment of membranous enzymes to a specific membrane system, i.e., plasma, organelle, or cell wall, is difficult. Progress in examining plant membrane-bound enzymes in detail has therefore been slow. Recently, however, density-gradient separation techniques, coupled with reliable marker enzymes, has been applied to a number of problems with some success (Moore et al., 1973). A further refinement is the employment of plant cells grown in suspension cultures under controlled conditions (Moore and Beevers, 1974). This material is reasonably uniform in age and development and has the added advantage of allowing the experimenter the opportunity of manipulating the milieu of the cell suspension under controlled conditions. With this background in mind we shall limit the contents of this chapter to a discussion of soluble and membrane-bound enzymes which participate in plant lipid metabolism.


Castor Bean Ricinoleic Acid Acyl Carrier Protein Spinach Chloroplast Fatty Acid Synthetase 


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  1. Abdelkader, A. B., Abdelkader, C., Damandre, C., and Mazliak, P., 1973, The oleyl CoA de-saturase of potato tubers. Enzymatic properties, intracellular localization and induction during “aging” of tuber slices, Eur. J. Biochem. 32:155–165.PubMedCrossRefGoogle Scholar
  2. Beevers, H., 1969, Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis, Ann. N.Y. Acad. Sci. 168:313.PubMedCrossRefGoogle Scholar
  3. Bieglmayer, C., Graf, J., and Ruis, H., 1973, Membranes of glyoxysomes from castor bean endosperm: Enzymes bound to purified-membrane preparations, Eur. J. Biochem. 37:553–562.PubMedCrossRefGoogle Scholar
  4. Bieglmayer, C., Nahler, G., and Ruis, H., 1974, Membranes of glyoxysomes from castor bean endosperm: Further studies on the membrane-bound enzymes of fatty acid degradation and of the glyoxylate cycle, Hoppe-Seyler’s Z. Physiol. Chem. 355:1121–1128.PubMedCrossRefGoogle Scholar
  5. Brenner, R. R., 1971, The desaturation step in the animal biosynthesis of polyunsaturated fatty acids, Lipids 6:567–575.PubMedCrossRefGoogle Scholar
  6. Burton, D., and Stumpf, P. K., 1966, Fat metabolism in higher plants XXXII. Control of plant acetyl CoA carboxylase activity, Arch. Biochem. Biophys. 117:604–614.CrossRefGoogle Scholar
  7. Douce, R., 1974, Site of biosynthesis of galactolipids in spinach chloroplasts, Science 183:852–853.PubMedCrossRefGoogle Scholar
  8. Galliard, T., and Stumpf, P. K., 1966, Fat metabolism in higher plants XXX. Enzymatic synthesis of ricinoleic acid by a microsomal preparation from developing Ricinus communis seeds, J. Biol. Chem. 241:5806–5812.PubMedGoogle Scholar
  9. Gurr, M. I., 1974, The biosynthesis of unsaturated fatty acids, in: Biochemistry of Lipids, Vol. 4 (T. W Goodwin, ed.), pp. 181–236, Butterworths, London.Google Scholar
  10. Harwood, J. L., 1974, Fatty acid biosynthesis by avocado pear, Lipids 9:850–854.PubMedCrossRefGoogle Scholar
  11. Harwood, J. L., Sodja, A., Stumpf, P. K., and Spurr, A. R., 1971, On the origin of oil droplets in maturing castor bean seeds, Ricinus communis, Lipids 6:851–854.CrossRefGoogle Scholar
  12. Jacobson, B. S., and Stumpf, P. K., 1972, Fat metabolism in higher plants LV. Acetate uptake and accumulation by class I and class II chloroplasts from Spinacia oleracea, Arch. Biochem. Biophys. 153:656–663.CrossRefGoogle Scholar
  13. Jacobson, B. S., Kannangara, C. G., and Stumpf, P. K., 1973, The elongation of medium chain trienoic acids to α-linolenic acid by a spinach chloroplast stroma system, Biochem. Biophys. Res. Commun. 52:1190–1198.PubMedCrossRefGoogle Scholar
  14. Jacobson, B. S., Jaworski, J. G., and Stumpf, P. K., 1974, Fat metabolism in higher plants LXII. Stearyl ACP desaturase from spinach chloroplasts, Plant Physiol. 54:484–486.PubMedCrossRefGoogle Scholar
  15. Jaworski, J. G., and Stumpf, P. K., 1974, Fat metabolism in higher plants LIX. Properties of a soluble stearyl ACP desaturase from maturing Carthamus tinctorius, Arch. Biochem. Biophys. 162: 158–165.PubMedCrossRefGoogle Scholar
  16. Jaworski, J. G., Goldschmidt, E. E., and Stumpf, P. K., 1974, Fat metabolism in higher plants LXIII. Properties of the palmityl ACP: stearyl ACP elongation system in maturing safflower seed extracts, Arch. Biochem. Biophys. 163:769–776.PubMedCrossRefGoogle Scholar
  17. Kannangara, C. G., and Stumpf, P. K., 1972, Fat metabolism in higher plants LIV. A procaryotic type acetyl CoA carboxylase in spinach chloroplasts, Arch. Biochem. Biophys. 152:83–91.PubMedCrossRefGoogle Scholar
  18. Marshall, M. O., and Kates, M., 1972, Biochim. Biophys. Acta 260:558–570.PubMedGoogle Scholar
  19. Moore, T. S., and Beevers, H., 1974, Isolation and characterization of organelles from soybean suspension cultures, Plant Physiol. 53:261–265.PubMedCrossRefGoogle Scholar
  20. Moore, T. S., Lord, J. M., Kagawa, T., and Beevers, H., 1973, Enzymes of phospholipid metabolism in the endoplasmic reticulum of castor bean endosperm, Plant Physiol. 52:50–53.PubMedCrossRefGoogle Scholar
  21. Morré, D. J. S., Nyquist, S., and Rivera, E., 1970, Lecithin biosynthetic enzymes of onion stem and the distribution of phosphorylcholine: cytidyl transferase among cell fractions, Plant Physiol. 45:800–804.PubMedCrossRefGoogle Scholar
  22. Mudd, J. B., 1967, Fat metabolism in plants, Annu. Rev. Plant Physiol 18:229–252.CrossRefGoogle Scholar
  23. Newgomb, E. H., and Stumpf, P. K., 1952, Fatty acid synthesis and oxidation in peanut cotyledons, in: Phosphorus Metabolism, Vol. II (W. O. McElroy and B. Glass, eds.), pp. 291–300, The Johns Hopkins Press, Baltimore.Google Scholar
  24. Pugh, E. L., and Kates, M., 1973, Desaturation of phosphatidylcholine and phosphatidyl ethanol-amine by a microsomal system from Candida lipolytica, Biochim. Biophys. Acta 316:305–316.Google Scholar
  25. Shine, W. E., and Stumpf, P. K., 1974, Recent studies on plant α-oxidation systems, Arch. Biochem. Biophys. 162:147–157.PubMedCrossRefGoogle Scholar
  26. Stumpf, P. K., 1975, Plant lipid metabolism, in: Plant Biochemistry (J. Bonner and J. Varner, eds.), Academic Press, New York, in press.Google Scholar
  27. Vagelos, P. R., 1974, Biosynthesis of saturated fatty acids, in: Biochemistry of Lipids, Vol. 4 (T. W. Goodwin, ed.), pp. 99–140, Butterworths, London.Google Scholar
  28. Vijay, I. K., and Stumpf, P. K., 1971, Fat metabolism in higher plants XLVI. Nature of the substrate and the product of oleyl CoA desaturase from Carthamus tinctorius, J. Biol. Chem. 246:2910–2917.PubMedGoogle Scholar
  29. Vijay, I. K., and Stumpf, P. K., 1972, Fat metabolism in higher plants XLVIII. Properties of oleyl CoA desaturase of Carthamus tinctorius, J. Biol. Chem. 247:360–366.PubMedGoogle Scholar
  30. Yang, S. F., and Stumpf, P. K., 1965, Biochim. Biophys. Acta 98:19–26.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • P. K. Stumpf
    • 1
  1. 1.Departments of Biochemistry and BiophysicsUniversity of CaliforniaDavisUSA

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