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The Effects of Lipid-Protein Interactions on the Kinetic Parameters of Microsomal UDP-Glucuronyltransferase

  • David Zakim
  • Donald A. Vessey

Abstract

The study of glucuronidation reactions began 100 years ago with the isolation of a conjugate of o-nitrotoluene from the urine of dogs. Acid hydrolysis of this conjugate yielded an acidic sugar as one of the products. An identical sugar was obtained on acid hydrolysis of a conjugate of chloral hydrate exreted in human urine. The formula of the reducing sugar was given correctly as
$$ {\left( {{\rm{CHOH}}} \right)_4}\left\{ {\begin{array}{*{20}{c}} {{\rm{CHO}}}\\ {{\rm{COOH}}} \end{array}} \right. $$
by Schmiedeberg and Meyer in 1879 (Smith and Williams, 1970). A wide variety of organic compounds are now known to be conjugated with glucuronic acid. Predominant among these are aromatic molecules containing phenolic and carboxylic groups which form O-ether and O-ester glucuronides, respectively. Several aromatic thiols are glucuronidated, and N-glucuronides also have been reported (Dutton, 1966). The donor of the glucuronic acid moiety in these reactions was identified as UDP-glucuronic acid (Dutton and Storey, 1953; Dutton, 1966).

Keywords

Liver Microsome Glucuronic Acid Microsomal Enzyme Microsomal Membrane Piperonyl Butoxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • David Zakim
    • 1
    • 2
  • Donald A. Vessey
    • 3
    • 4
  1. 1.Department of MedicineUniversity of California Medical CenterSan FranciscoUSA
  2. 2.Molecular Biology DivisionVeterans Administration HospitalSan FranciscoUSA
  3. 3.Department of Biochemistry and BiophysicsUniversity of California Medical CenterSan FranciscoUSA
  4. 4.Molecular Biology DivisionVetrans Administration HospitalSan FranciscoUSA

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