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The Effects of Lipid-Protein Interactions on the Kinetic Parameters of Microsomal UDP-Glucuronyltransferase

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Book cover The Enzymes of Bioligical Membranes

Abstract

The study of glucuronidation reactions began 100 years ago with the isolation of a conjugate of o-nitrotoluene from the urine of dogs. Acid hydrolysis of this conjugate yielded an acidic sugar as one of the products. An identical sugar was obtained on acid hydrolysis of a conjugate of chloral hydrate exreted in human urine. The formula of the reducing sugar was given correctly as

$$ {\left( {{\rm{CHOH}}} \right)_4}\left\{ {\begin{array}{*{20}{c}} {{\rm{CHO}}}\\ {{\rm{COOH}}} \end{array}} \right. $$

by Schmiedeberg and Meyer in 1879 (Smith and Williams, 1970). A wide variety of organic compounds are now known to be conjugated with glucuronic acid. Predominant among these are aromatic molecules containing phenolic and carboxylic groups which form O-ether and O-ester glucuronides, respectively. Several aromatic thiols are glucuronidated, and N-glucuronides also have been reported (Dutton, 1966). The donor of the glucuronic acid moiety in these reactions was identified as UDP-glucuronic acid (Dutton and Storey, 1953; Dutton, 1966).

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Authors and Affiliations

  1. Department of Medicine, University of California Medical Center, San Francisco, California, USA

    David Zakim

  2. Molecular Biology Division, Veterans Administration Hospital, San Francisco, California, USA

    David Zakim

  3. Department of Biochemistry and Biophysics, University of California Medical Center, San Francisco, California, USA

    Donald A. Vessey

  4. Molecular Biology Division, Vetrans Administration Hospital, San Francisco, California, USA

    Donald A. Vessey

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  1. David Zakim
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  1. Department of Biochemistry, School of Medicine, Saint Louis University, USA

    Anthony Martonosi

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© 1976 Plenum Press, New York

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Zakim, D., Vessey, D.A. (1976). The Effects of Lipid-Protein Interactions on the Kinetic Parameters of Microsomal UDP-Glucuronyltransferase. In: Martonosi, A. (eds) The Enzymes of Bioligical Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2655-7_12

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  • DOI: https://doi.org/10.1007/978-1-4684-2655-7_12

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