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Germ line basis for antibody diversity

  • A. R. Williamson
  • E. Premkumar
  • M. Shoyab
Part of the Faseb Monographs book series (FASEBM, volume 3)

Abstract

Each antibody polypeptide chain is the product of a gene pair comprising one constant (C) gene coding for that portion of the chain common to all chains of the same type and one variable (V) gene coding for the sequence unique to each chain. Previous evidence indicates that the haploid genome has a single copy of each distinct C gene and that for expression a gene pair is formed with any one of a family of V genes present in the same haploid genome. Hybridization of purified mRNA coding for immunoglobulin heavy chain (mRNA-H) with a vast excess of DNA confirms the existence of a single C gene of each type and multiple V genes. A large number (of the order of 104) of V genes would be consistent with the hybridization results. This suggests considerable V gene redundancy which is a predictable property of a multiple V gene family maintained by expansion and contraction mechanisms. The mRNA-H used in these hybridization studies was isolated by a specific interaction with immunoglobulin. The same method has also been used to isolate a nuclear precursor of mRNA-H. Identification of this precursor strengthens the evidence for the direct joining of the V and C gene pair at the DNA level prior to transcription.—Williamson, A. R., E. Premkumar And M. Shoyab. Germ line basis for antibody diversity. Federation Proc. 34: 28–32, 1975.

Keywords

Gene Pair Versus Gene Haploid Genome Immunoglobulin Heavy Chain Untranslated Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Birshtein, B. K., And J. J. Cebra. Biochemistry 10: 4930, 1971.PubMedCrossRefGoogle Scholar
  2. 2.
    Bishop, J. O. Gene transcription in reproductive tissue. Karolinska Symposia on Research Methods in Reproductive Endocrinology, 5th Symposium, p. 247.Google Scholar
  3. 3.
    Blomberg, B., M. Cohn, W. Geckeler, W. Raschke, R. Riblet And M. Weigert. In: The Immune System: Genes, Receptors, Signals, edited by C. F. Fox, E. E. Sercarz and A. R. Williamson. New York: Academic, 1974.Google Scholar
  4. 4.
    Blomberg, B., W. R. Geckeler And M. Weigert. Science 177: 178, 1972.PubMedCrossRefGoogle Scholar
  5. 5.
    Britten, R. J., And D. E. Kohne. Science 161: 529, 1968.PubMedCrossRefGoogle Scholar
  6. 6.
    Brown, D. D., P. C. Wensink And E. Jordan. J Mol. Biol. 63: 57, 1972.PubMedCrossRefGoogle Scholar
  7. 7.
    Capra, J. D., R. L. Wasserman And J. M. Kehoe. J. Exptl. Med. 138: 410, 1973.CrossRefGoogle Scholar
  8. 8.
    Dreyer, W. J., And J. C. Bennett. Proc. Natl. Acad. Sci. U.S. 54: 864, 1965.CrossRefGoogle Scholar
  9. 9.
    Eichmann, K., And C. Berek. European J. Immunol. 3: 599, 1973.CrossRefGoogle Scholar
  10. 10.
    Farquhar, M. N., And B. J. Mccarthy. Biochemistry 12: 4113, 1973.CrossRefGoogle Scholar
  11. 11.
    Franek, F. FEBS Letters 8: 269, 1970.PubMedCrossRefGoogle Scholar
  12. 12.
    Francis, S. H., R. G. Q. Leslie, L. Hood And H. N. Eisen. Proc. Natl. Acad. Sci. U.S. 71: 1123, 1974.CrossRefGoogle Scholar
  13. 13.
    Gilbert, W., And D. Dressler. Cold Spring Harbor Symp. Quant. Biol. 33: 473, 1968.PubMedCrossRefGoogle Scholar
  14. 14.
    Hood, L., K. Eichmann, H. Lackland, R. M. Krause And J. J. Ohms. Nature 228: 1040, 1970.CrossRefGoogle Scholar
  15. 15.
    Hood, L., D. Mckean, V. Farnsworth And M. Potter. Biochemistry 12: 741, 1973.PubMedCrossRefGoogle Scholar
  16. 16.
    Imanishi, T., And O. Makela. European J. Immunol 3: 323, 1973.CrossRefGoogle Scholar
  17. 17.
    Inman, J. K. In: The Immune System: Genes, Receptors, Signals, edited by C. F. Fox, E. E. Sercarz and A. R. Williamson. New York: Academic, 1974.Google Scholar
  18. 18.
    Kreth, H. W., And A. R. Williamson. European J. Immunol. 3: 141, 1973.CrossRefGoogle Scholar
  19. 19.
    Kuettner, M. G., A. L. Wang And A. Nisonoff. J. Exptl. Med. 135: 579, 1972.CrossRefGoogle Scholar
  20. 20.
    Melli, M., C. Whitfield, K. V. Rao, M. Richardson And J. O. Bishop. Nature New Biol. 231: 8, 1971.PubMedGoogle Scholar
  21. 21.
    Miller, L., And J. B. Gurdon. Nature 227: 1108, 1970.PubMedCrossRefGoogle Scholar
  22. 22.
    Miller, L., And J. Knowland. J. Mol. Biol. 53: 329, 1970.PubMedCrossRefGoogle Scholar
  23. 23.
    Milstein, C. Nature 216: 330, 1967.PubMedCrossRefGoogle Scholar
  24. 24.
    Milstein, C., And J. R. L. Pink. Progr. Biophys. Mol. Biol. 21: 211, 1970.CrossRefGoogle Scholar
  25. 25.
    Niall, H. D., And P. Edman. Nature 216: 262, 1967.PubMedCrossRefGoogle Scholar
  26. 26.
    Premkumar, E., M. Shoyab And A. R. Williamson. Proc. Natl. Acad. Sci. U.S. 71: 99, 1974.CrossRefGoogle Scholar
  27. 27.
    Ritossa, F. M. Proc. Natl. Acad. Sci. U.S. 60: 509, 1968.CrossRefGoogle Scholar
  28. 28.
    Sher, A., And M. Cohn. European J. Immunol. 2: 319, 1972.CrossRefGoogle Scholar
  29. 29.
    Smith, G. P., L. Hood And W. M. Fitch. Ann. Rev. Biochem. 40: 969, 1971.CrossRefGoogle Scholar
  30. 30.
    Stevens, R. H. European J. Biocherà. 42: 553, 1974.CrossRefGoogle Scholar
  31. 31.
    Stevens, R. H., And A. R. Williamson. Proc. Natl. Acad. Sci. U.S. 70: 1127, 1973.CrossRefGoogle Scholar
  32. 32.
    Stevens, R. H., And A. R. Williamson. Nature New Biol. 245: 101, 1973.PubMedCrossRefGoogle Scholar
  33. 33.
    Stevens, R. H., And A. R. Williamson. J. Mol. Biol. 78: 505, 1973.PubMedCrossRefGoogle Scholar
  34. 34.
    Stevens, R. H., And A. R. Williamson. J. Mol. Biol. 78: 517, 1973.PubMedCrossRefGoogle Scholar
  35. 35.
    Stevens, R. H., And A. R. Williamson. Contemporary Topics in Molecular Immunology In press.Google Scholar
  36. 36.
    Stevens, R. H., And A. R. Williamson. Nature 239: 143, 1972.PubMedCrossRefGoogle Scholar
  37. 37.
    Tartof, K. D. Science 171: 294, 1971.PubMedCrossRefGoogle Scholar
  38. 38.
    Tartof, K. D. Proc. Natl. Acad. Sci. U.S. 71: 1272, 1974.CrossRefGoogle Scholar
  39. 39.
    Todd, C. W., And F. R. Inman. Immunochemistry 4: 407, 1967.CrossRefGoogle Scholar
  40. 40.
    Williamson, A. R. Biochem. J. 130: 325, 1972.PubMedGoogle Scholar

Copyright information

© Federation of American Societies 1975

Authors and Affiliations

  • A. R. Williamson
    • 1
  • E. Premkumar
    • 1
  • M. Shoyab
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of California, Los Angeles School of MedicineLos AngelesUSA

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