Age-related changes in glucocorticoid binding by rat splenic leukocytes: possible cause of altered adaptive responsiveness
Splenic leukocytes of senescent rats (24–26 mo) exhibit a 60% reduction in cortisol-induced inhibition of 3H-uridine uptake when compared to mature adult animals (12–14 mo). The degree of inhibition is directly proportional to cortisol dosage up to 2 x 10 −6M. Specific binding of physiological (nanomolar) concentrations of 3H-cortisol by leukocytic cytosol macromolecules is reduced by over 40% in the older animals. Moreover, Scatchard analyses reveal 60% fewer specific glucocorticoid binding sites in the cytosols of cells from the senescent rats. Such analyses were performed in vitro at 0 C to eliminate metabolism of steroids. In addition, 3H-dexamethasone was used instead of 3H-cortisol to eliminate binding to plasma (or serum) transcortin. Inhibition of 3H-uridine uptake requires specific glucocorticoid binding. The degree of inhibition at varying glucocorticoid dosages is proportional to the amount of specific binding to cytoplasmic macromolecules. Thus, age-related reduction in specific glucocorticoid binding sites may be at least partially responsible for altered responsiveness of splenic leukocytes to these hormones.Roth G. S. Age-related changes in glucocorticoid binding by rat splenic leukocytes: possible cause of altered adaptive responsiveness. Federation Proc. 34: 183–185, 1975.
KeywordsScatchard Analysis Cortisol Dosage Splenic Leukocyte Glucocorticoid Binding Individual Spleen
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