The three-dimensional conformation of an enzyme has an important effect on its catalytic action. Consequently, when immobilizing an enzyme, it is necessary to use such methods and chemicals that the functional tertiary structure will not be affected. Physical entrapment of an enzyme in a gel lattice is an immobilization method in which no modification of the amino acid residues is needed, and which offers the advantage of reaction conditions usually so mild that few significant changes in the enzyme structure occur. A great advantage lies in the fact that the presence of protective and stabilizing agents does not affect the yield of the entrapped material. The method has a broad applicability to most enzymes, purified as well as crude extracts, to whole cells (e.g., microorganisms) and even to culture broths containing the desired enzyme.


Acrylic Acid Immobilize Enzyme Glucose Oxidase Acrylic Monomer Acrylic Copolymer 
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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Ann-Christin Koch-Schmidt
    • 1
  1. 1.Biochemistry IIUniversity of LundLundSweden

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