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Covalent Linkage: III. Immobilization of Enzymes by Intermolecular Cross-Linking

  • Oskar R. Zaborsky

Abstract

A very versatile chemical method for immobilizing enzymes and other proteins is intermolecular cross-linking with multifunctional reagents. The method can produce enzyme conjugates of diverse physical character depending on the conditions employed. Thus, conjugates can be prepared that are water-soluble or water-insoluble; granular, gel-like, or filamentous in nature; or that are pure protein. It is reliable and inexpensive.

Keywords

Native Enzyme Covalent Linkage Enzyme Conjugate Ethyl Chloroformate Bifunctional Reagent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Oskar R. Zaborsky
    • 1
  1. 1.National Science FoundationUSA

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