Strategy for Enzyme Therapy: Immobilization in Hypoallergenic Gel Versus Entrapment in Red Blood Cell

  • Stuart Updike


The polyacrylamide gel immobilization system (Hicks and Updike, 1966; Updike and Hicks, 1967) provides a chemically inert and apparently nontoxic carrier of the enzyme. Polyacrylamide has a hydrocarbon backbone with amide groups as shown in Figure 1, and therefore is devoid of what normally are considered antigenic determinants (Rabat, 1966). To prevent excessive swelling of the gel, a cross-linking agent, N,N’-methylene-bisarcylamide is introduced, as shown in Figure 1. Purified enzyme can be entrapped in the gel during photopolymerization at room temperature. The polymerization reaction is exothermic, therefore, the reaction vial is placed in an ice bath as needed to dissipate heat. The gel is then mechanically fragmented and wet-sieved to provide the right particle size.


Average Pore Size Enzyme Therapy Therapeutic Enzyme Original Article Series Asparagine Level 
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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Stuart Updike
    • 1
  1. 1.Department of MedicineUniversity of Wisconsin Center for Health SciencesMadisonUSA

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