In nature, most enzymes and proteins are located inside biological cells. Inside cells, most enzymes and proteins are further subdivided into smaller immobilized systems of intracellular organelles or intracellular membrane systems. Enzyme and protein research and its application have gone through interesting stages (Table 1). Stage I included extensive research into the extraction, purification, and crystallization of enzymes from biological cells and other sources. Enzymes obtained this way were used for detailed studies of enzyme kinetics.


Immobilize Enzyme Enzyme Kinetic Biological Cell Covalent Linkage Intracellular Environment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • T. M. S. Chang
    • 1
  1. 1.Departments of Physiology and MedicineMcGill UniversityMontrealCanada

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