Fractionation of Diverse Steroid-Binding Proteins: Basic and Clinical Applications

  • Merry R. Sherman
  • Lorraine K. Miller
Part of the Current Topics in Molecular Endocrinology book series (CTME, volume 4)


Two types of diversity in steroid-binding proteins have become apparent: 1) the presence of several intracellular receptors and/or serum steroid-binding components in a tissue; and 2) the existence of multiple forms of individual receptors. Resolution of these components is clearly essential to understanding the mechanisms of action of one or more steroids in a target cell. The techniques that can be used to fractionate such mixtures and characterize the constituents include analytical gel filtration, Polyacrylamide gel electrophoresis, preparative ion exchange filtration and polyamine precipitation, in addition to density gradient centrifu-gation. Applications of these techniques to a clinical problem, the influence of steroids on breast cancer, and a fundamental problem, the subunit structure of chick oviduct progesterone receptors, will be described in this chapter.


Steroid Binding Globulin Label Complex Undiluted Serum Total Liquid Volume Protamine Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.








DHT or dihydrotestosterone


E2 or estradiol





human serum albumin; SBG, sex steroid binding globulin (testosterone-estradiol binding globulin)


N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid


10mM Tes, 12mM thioglycerol, pH 7.4


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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Merry R. Sherman
    • 1
  • Lorraine K. Miller
    • 1
  1. 1.Memorial Sloan-Kettering Cancer CenterNew YorkUSA

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