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Abstracts

  • Fernand Labrie
  • Joseph Meites
  • Georges Pelletier
Part of the Current topics in Molecular Endocrinology book series (CTME, volume 3)

Abstract

The growing evidence for a correlation between the hormonal dependence of neoplastic tissues and the presence of specific hormone receptors led us to investigate the possible presence of specific progesterone receptors in dimethyl benzanthracene (DMBA)-induced mammary tumors in the rat. After homogeneisation in 3 vol (w/v) of 25 mM Tris-HC1 (pH 7.4), 1.5 mM EDTA, 10 mM thioglycerol and 10% glycerol (buffer A), the 105,000 xg super-natant was used for binding studies with the highly potent syn-thetic progestin [3H] R 5020 (17, 21-dimethyl-l9-nor-pregna-4, 9-diene-3, 20-dione). Specificity of [3H] R 5020 binding was studied by both sucrose gradients and charcoal adsorption. As evidenced by sucrose gradient analysis, specific binding of [3H] R 5020 is associated with components migrating at 6–7S and 4–5S. Unspecific binding of the synthetic progestin at 4S is eliminated by pretreatment with dextran-coated charcoal. [3H] R 5020 binding is easily displaced by unlabeled R 5020, progesterone, norgestreal, chlormethindrone and norethindrone. 17β-estradiol, diethylstilbestrol, testosterone, dihydrotestosterone, dexame-thasone and cortisol have little, if any effect on [3H] R 5020 binding.

Keywords

Growth Hormone Thyrotropin Release Hormone Anterior Pituitary Cell Estradiol Benzoate Estradiol Benzoate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Fernand Labrie
    • 1
  • Joseph Meites
    • 2
  • Georges Pelletier
    • 1
  1. 1.Centre Hospitalier de l’Université LavalCanada
  2. 2.Michigan State UniversityEast LansingUSA

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