Immunohistochemical Localization of Hypothalamic Hormones at the Electron Microscope Level

  • Georges Pelletier
Part of the Current topics in Molecular Endocrinology book series (CTME, volume 3)


Adenohypophyseal activity is under the control of neurohormones released from the hypothalamus and carried to their specific sites of action, the pituitary cells, by a portal blood system. While it was well established that hypothalamic regulatory hormones are present in high concentrations in the median eminence (Ishii, 1970; Krulich et al., 1971), it has been only recently reported that TRH and somatostatin are present in some extra-hypo-thalamic areas (Brownstein et al., 1974; Brownstein et al., 1975). A direct inhibitory effect of somatostatin on the secretion of insulin, glucagon (Koerker et al., 1974) and gastrin (Hayes et al., 1975) has also been reported thus suggesting that this neurohormone could be possibly produced and/or stored in the pancreas and gastrointestinal tract. In order to gain a better understanding of the mechanisms of packaging and secretion of LH-RH and somatostatin in the brain and target tissues detailed immunohistochemical studies were performed on the localization of these peptides.


Secretory Granule Nerve Ending Pineal Gland Ependymal Cell Median Eminence 
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  1. Arimura, A., Sato, H., Coy, D.H. and Schally, A.V. (1975) Proc. Soc. Exp. Biol. Med. 148, 784–788.PubMedGoogle Scholar
  2. Baker, D.L., Dermody, W.C. and Reel, J.R. (1974) Am. J. Anat. 139, 129–134.PubMedCrossRefGoogle Scholar
  3. Barry, J. and Dubois, M.P. (1973) Ann. Endocr. (Paris) 34, 735–742.Google Scholar
  4. Brownstein, M.J., Palkovits, M., Saavedra, J.M., Bassiri, P.M. and Utiger, R.D. (1974) Science 185, 267–269.PubMedCrossRefGoogle Scholar
  5. Brownstein, M., Arimura, A., Sato, M., Schally, A.V. and Kizer, J.S. (1975) Endocrinology 96, 1456–1461.PubMedCrossRefGoogle Scholar
  6. Dubé, D., Leclerc, R., Pelletier, G., Arimura, A., Schally, A.V., (1975) Cell. Tiss. Res., in press.Google Scholar
  7. Dupont, A., Labrie, F., Pelletier, G., Puviani, R., Coy, D.H., Coy, E.J. and Schally, A.V. (1974) Neuroendocrinology 16, 65–73.PubMedCrossRefGoogle Scholar
  8. Forssman, W.G., Orci, L., Pidet, R., Renold, A.E. and Rouiller, C. (1969) J. Cell. Biol. 40, 698–715.CrossRefGoogle Scholar
  9. Hayes, J.R., Johnson, D.G., Koerker, D. and Williams, R.H. (1975) 96, 1374–1376.Google Scholar
  10. Hökfelt, T., Efendic, S., Johansson, O., Luft, R. and Arimura, A. (1974) Brain Res. 80, 165–169.PubMedCrossRefGoogle Scholar
  11. Ishii, S. (1970) Endocrinology 86, 207–216.PubMedCrossRefGoogle Scholar
  12. Köerker, D.J., Ruch, W., Chideckel, E., Palmer, J., Goodner, C.J., Ensinck, J. and Gale, C.C. (1974) Science 184, 482–484.PubMedCrossRefGoogle Scholar
  13. Kordon, C., Kerdelhué, B., Patton, E. and Jutisz, M. (1974) Proc. Soc. Exp. Biol. Med. 147, 122–127.PubMedGoogle Scholar
  14. Krulich, L., Quijada, M., Illmer, P., McCann, S.M. (1971) Proc. Int. Union. Physiol. 9, 326.Google Scholar
  15. Leclerc, R. and Pelletier, G. (1974) Am. J. Anat. 140, 583–588.PubMedCrossRefGoogle Scholar
  16. Pelletier, G., Labrie, F., Puviani, R., Arimura, A. and Schally, A.V. (1974a) Endocrinology 95, 314–317.PubMedCrossRefGoogle Scholar
  17. Pelletier, G., Labrie, F., Arimura, A. and Schally, A.V. (1974b) Am. J. Anat. 140, 445–450.PubMedCrossRefGoogle Scholar
  18. Pelletier, G., Leclerc, R., Dubé, D., Labrie, F., Puviani, R., Arimura, A.V., Schally, A.V. (1975a) Am. J. Anat. 142, 297–301.CrossRefGoogle Scholar
  19. Pelletier, G., Leclerc, R., Arimura, A. and Schally, A.V. (1975b) J. Histochem. Cytochem., 9, 699–701.CrossRefGoogle Scholar
  20. Polak, J.M., Grimelis, L., Pearse, A.G.E. and Bloom, S.R. (1975) The Lancet 1, 1220–1222.CrossRefGoogle Scholar
  21. Setalo, G., Vigh, S., Schally, A.V., Arimura, A. and Flerko, B. (1975) Endocrinology 96, 135–142.PubMedCrossRefGoogle Scholar
  22. Sternberger, L.A. (1974) Immunocytochemistry, Prentice-Hall Inc., Englewood Cliffs, N.J.Google Scholar
  23. Zimmerman, E.A., Hsu, K.G., Ferrin, M. and Kozlowski, G.P. (1974) Endocrinology 95, 1–8.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Georges Pelletier
    • 1
  1. 1.Medical Research Council Group in Molecular EndocrinologyCentre Hospitalier de l’Université LavalCanada

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