Immunohistochemical Localization of Hypothalamic Hormones at the Electron Microscope Level
Adenohypophyseal activity is under the control of neurohormones released from the hypothalamus and carried to their specific sites of action, the pituitary cells, by a portal blood system. While it was well established that hypothalamic regulatory hormones are present in high concentrations in the median eminence (Ishii, 1970; Krulich et al., 1971), it has been only recently reported that TRH and somatostatin are present in some extra-hypo-thalamic areas (Brownstein et al., 1974; Brownstein et al., 1975). A direct inhibitory effect of somatostatin on the secretion of insulin, glucagon (Koerker et al., 1974) and gastrin (Hayes et al., 1975) has also been reported thus suggesting that this neurohormone could be possibly produced and/or stored in the pancreas and gastrointestinal tract. In order to gain a better understanding of the mechanisms of packaging and secretion of LH-RH and somatostatin in the brain and target tissues detailed immunohistochemical studies were performed on the localization of these peptides.
KeywordsSecretory Granule Nerve Ending Pineal Gland Ependymal Cell Median Eminence
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