Characteristics of Soluble Gonadotropin Receptors for LH and hCG

  • Maria L. Dufau
  • E. Charreau
  • D. Ryan
  • K. J. Catt
Part of the Current Topics in Molecular Endocrinology book series (CTME, volume 1)


In order initiate studies on the molecular properties of the gonadotropin receptor sites for luteinizing hormone, subcellular fractions of testis and ovarian homogenates with specific binding affinity for LH and hCG have been treated with non-ionic deter­gents such as ‘Triton X-100’ and ‘Lubrol’ to obtain “solubilized” receptor preparations which are suitable for binding studies and physico-chemical characterization (1–3).


Polyethylene Glycol Soluble Receptor Blue Dextran Sucrose Density Gradient Centrifugation Free Receptor 


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  1. 1.
    Dufau, M. L. and Catt, K. J., Nature New Biol. 242: 246, 1973.PubMedGoogle Scholar
  2. 2.
    Dufau, M. L., Charreau, E. H. and Catt, K J., J. Biol. Chem. 248: 6973, 1973.PubMedGoogle Scholar
  3. 3.
    Dufau, M. L., Charreau, E. H., Ryan, D. and Catt, K. J., FEBS Letters 39: 149, 1974.PubMedCrossRefGoogle Scholar
  4. 4.
    Dufau, M. L., Tsuruhara, T. and Catt, K. J., Biochim. Biophys. Acta 278: 281, 1972.Google Scholar
  5. 5.
    Charreau, E. H., Dufau, M. L. and Catt, K. J., Biol. Chem. 294: 4189, 1974.Google Scholar
  6. 6.
    Swart, A.C.W. and Hemker, H. C. Biochim. Biophys. Acta 222: 692, 1970.CrossRefGoogle Scholar
  7. 7.
    Staal, G.E. J., Koster, J. F., Kamp, H., Millingen Boersma, L. and Veerger, C., Biochim. Biophys. Acta 227: 86, 1971.Google Scholar
  8. 8.
    Laurent, T. C. and Killander, J., J. Chromatogr. 14: 317, 1964.CrossRefGoogle Scholar
  9. 9.
    Siegel, L. M. and Monty, K. J., Biochim. Biophys. Acta 112: 346, 1966.CrossRefGoogle Scholar
  10. 10.
    Rodbell, M., Krans, H.M.J., Pohl, S. L. and Birnbaumer, L., J. Biol. Chem. 246: 1861, 1971.PubMedGoogle Scholar
  11. 11.
    Barden, N., and Labrie, F., J. Biol. Chem. 248: 7601, 1973.PubMedGoogle Scholar
  12. 12.
    Simpkins, H., Tay, S., and Panko, E., Biochemistry 10: 3579 (1971).PubMedCrossRefGoogle Scholar
  13. 13.
    Dufau, M. L., Ryan, D. and Catt, K. J., Biochim. Biophys. Acta 343: 417, 1974.CrossRefGoogle Scholar
  14. 14.
    Belisario, R., Carlsen, O. P., Swaminathan, N., Bahl, O.. P., J. Biol. Chem. 248: 6180, 1973.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Maria L. Dufau
    • 1
  • E. Charreau
    • 1
  • D. Ryan
    • 1
  • K. J. Catt
    • 1
  1. 1.Reproduction Research Branch National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaUSA

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