FSH Receptors in Rat Testes: Chemical Properties and Solubilization Studiies

  • V. K. Bhalla
  • Leo E. ReichertJr.
Part of the Current Topics in Molecular Endocrinology book series (CTME, volume 1)


The interaction of protein hormones with specific receptors is believed to initiate a series of events at the molecular level leading ultimately to a physiologically significant response. We have previously demonstrated the presence luteinizing hormone (LH) specific receptors in Leydig cell homogenates (1, 2) and follicle stimulating hormone (FSH) specific receptors in seminiferous tubule homogenate (3) of mature rat testes. These studies allowed development of tissue receptor assays for hLH (2, 4) and hFSH (5) suitable for measurement of gonadotropin activities in pituitary fractions as well as for various types of structure-function studies. We have extended our earlier binding studies with FSH (3) and in this report describe our recent observations on the interaction of 125I-hFSH with testicular receptors as well as preliminary studies on the chemical properties of the receptor and attempts directed towards its solubilization.


Luteinizing Hormone Follicle Stimulate Hormone Leydig Cell Seminiferous Tubule Follicle Stimulate Hormone Receptor 
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  1. 1.
    Leidenberger, F., and Reichert, L. E., Jr. Endocrinology 91: 135, 1972.PubMedCrossRefGoogle Scholar
  2. 2.
    Reichert, L. E., Jr., Leidenberger, F., and Trowbridge, C. G. Recent Progr. Hor.m. Res. 29: 497, 1973.Google Scholar
  3. 3.
    Bhalla, Vinod K., and Reichert, L. E., Jr., J. Biol. Chem. 249: 43, 1973.Google Scholar
  4. 4.
    Leidenberger, F., and Reichert, L. E., Jr., Endocrinology 91: 901, 1972.PubMedCrossRefGoogle Scholar
  5. 5.
    Reichert, L. E., Jr., and Bhalla, Vinod K., Endocrinology in press.Google Scholar
  6. 6.
    Greenwood, F. C., Hunter, W. M., and Glover, J. S., Biochem. J. 89: 114, 1963.PubMedGoogle Scholar
  7. 7.
    Butt, W. R., Acta Endocr. Suppl. 142: 13, 1969.Google Scholar
  8. 8.
    Miyachi, Y., Vaitukaitis, J., Nieschlag, E., and Lipsett, M. B., J. Clin. Endocrinol. Metab. 34: 23, 1972.CrossRefGoogle Scholar
  9. 9.
    Miyachi, Y., and Chrambach, A., Biochem. Biophys. Res. Comm. 46: 1213, 1972.CrossRefGoogle Scholar
  10. 10.
    Mancini, R. E., Costra, A., and Seiguer, A. C., Histochem. Cytochem. 15: 516, 1967.CrossRefGoogle Scholar
  11. 11.
    Kuehl, F. A., Jr., Patanelli, D. J., Tarnoff, J., and Humes, J. L., Biol. Rep. 2: 154, 1970.CrossRefGoogle Scholar
  12. 12.
    Christensen, A. K., and Mason, N. R., Endocrinology 76: 646, 1965.PubMedCrossRefGoogle Scholar
  13. 13.
    Dufau, M. L., Charreau, E. H., and Catt, K. J., J. Biol. Chem. 248: 6973, 1973.PubMedGoogle Scholar
  14. 14.
    Rodbell, M., Birnbaumer, L., and Pohl, S. L., in The Role of Adenyl Cyclase and cAMP in Biological Systems“, Rall, T. M., Rodbell, M., and Condliffe, P. (eds.), U. S. Govt. Printing Office, Washington, D. C., p. 59, 1969.Google Scholar
  15. 15.
    Lefkowitz, R. J., and Levey, G. S., Life Sciences 11: 821, 1972.CrossRefGoogle Scholar
  16. 16.
    Makino, S., Reynolds, J. A., and Tanford, C., J. Biol. Chem. 248: 4926, 1973.PubMedGoogle Scholar
  17. 17.
    Neer, E. J., J. Biol. Chem. 248: 3742, 1973.PubMedGoogle Scholar
  18. 18.
    Johnson, R. A., and Sutherland, E., J. Biol. Chem. 248: 5114, 1973.PubMedGoogle Scholar
  19. 19.
    Cuatrecasas, P., Proc. Nat. Acad. Sci. US 69: 318, 1972.CrossRefGoogle Scholar
  20. 20.
    Allison, A. C., and Hartree, E. F., J. Reprod. Fert. 21: 501, 1970.CrossRefGoogle Scholar
  21. 21.
    Karlin, A., Fed. Proceedings 32: 1847, 1973.Google Scholar
  22. 22.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem. 193: 265, 1951.PubMedGoogle Scholar
  23. 23.
    Cuatrecasas, P., Fed. Proceedings 32: 1838, 1973.Google Scholar
  24. 24.
    Cuatrecasas, P., J. Biol. Chem. 246: 6532, 1971.PubMedGoogle Scholar
  25. 25.
    Lee, C. Y., and Ryan, R. J., Fifth Annual Meeting of the Endocrine Society 280 (Abstract), 1972.Google Scholar
  26. 26.
    Butt, W. R., Ryle, M., and Shirley, A., J. Endocr. 58: 275, 1973.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • V. K. Bhalla
    • 1
  • Leo E. ReichertJr.
    • 1
  1. 1.Division of Basic Health SciencesEmory UniversityAtlantaUSA

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