FSH Receptors in Rat Testes: Chemical Properties and Solubilization Studiies
The interaction of protein hormones with specific receptors is believed to initiate a series of events at the molecular level leading ultimately to a physiologically significant response. We have previously demonstrated the presence luteinizing hormone (LH) specific receptors in Leydig cell homogenates (1, 2) and follicle stimulating hormone (FSH) specific receptors in seminiferous tubule homogenate (3) of mature rat testes. These studies allowed development of tissue receptor assays for hLH (2, 4) and hFSH (5) suitable for measurement of gonadotropin activities in pituitary fractions as well as for various types of structure-function studies. We have extended our earlier binding studies with FSH (3) and in this report describe our recent observations on the interaction of 125I-hFSH with testicular receptors as well as preliminary studies on the chemical properties of the receptor and attempts directed towards its solubilization.
KeywordsLuteinizing Hormone Follicle Stimulate Hormone Leydig Cell Seminiferous Tubule Follicle Stimulate Hormone Receptor
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