Fluorescence Spectroscopy with On-Line Computers

Methods and Instrumentation
  • John E. Wampler
Part of the Modern Analytical Chemistry book series (MOAC, volume 1)


In spite of the so-called computer revolution, the general acceptance of the digital computer by research scientists as a laboratory tool has been slow in coming. All too often uninitiated members of the scientific community look upon the in-lab, on-line computer system as an overly complex, expensive instrument of limited performance. These misconceptions arise in part from not recognizing the enormous strides modern electronic technology has made in size reduction and increased performance. A modern minicomputer, while no bigger than a suitcase, contains as much memory, operates faster, and has more input-output capabilities than many of the early large-scale machines, all with a purchase price around $5000. Indeed, each year the performance of these machines increases, the size decreases, and the cost goes down.


Step Motor Synchronous Motor Paper Tape Optomechanical System Lamp Pulse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R. J. DeSa and J. E. Wampler, Appl. Spectrosc. 27, 279 (1973).CrossRefGoogle Scholar
  2. 2.
    U. B. Renalder, H. Känzig, and U. P. Wild, Appl Spectrosc. 28, 45 (1974).CrossRefGoogle Scholar
  3. 3.
    R. J. DeSa and Q. H. Gibson, Comp. Biomed. Res. 2, 494 (1969).CrossRefGoogle Scholar
  4. 4.
    C. Klopfenstein, P. Jost, and O. H. Griffith, in Computers in Chemical and Biochemical Research, C. Klopfenstein and C. Wilkins, eds. (Academic Press, New York, 1972), Vol. 1, p. 176.Google Scholar
  5. 5.
    J. W. Frazier, Anal. Chem. 40 (8), 26A (1968).CrossRefGoogle Scholar
  6. 6.
    J. A. Murphy, Modern Data, August 1974, 46 (1974).Google Scholar
  7. 7.
    C. J. Fielden, J. Phys. E 6, 1161 (1973).CrossRefGoogle Scholar
  8. 8.
    R. E. Dessy and J. Titus, Anal. Chem. 46, 294A (1974).Google Scholar
  9. 9.
    S. P. Perone, Anal. Chem. 43, 1288 (1971).CrossRefGoogle Scholar
  10. 10.
    S. P. Perone and D. O. Jones, Digital Computers in Scientific Instrumentation (McGraw-Hill, New York, 1973).Google Scholar
  11. 11.
    R. J. DeSa, in Computers in Chemical and Biochemical Research, C. Klopfenstein and C. Wilkins, eds. (Academic Press, New York, 1972), Vol. 1, p. 83.Google Scholar
  12. 12.
    K. Gruber, J. Forrer, E. Zopfi, and H. H. Günthard, J. Phys. E 6, 666 (1973).CrossRefGoogle Scholar
  13. 13.
    J. G. Graeme, G. E. Tobey, and L. P. Huelsman, Operational Amplifiers: Design and Applications (McGraw-Hill, New York, 1971).Google Scholar
  14. 14.
    D. M. Anderson, Basic Computer Programming (Appleton-Century-Crofts, New York, 1974).Google Scholar
  15. 15.
    H. D. Pecknam, Computers, BASIC, and Physics (Addison-Wesley, Reading, Mass., 1971).Google Scholar
  16. 16.
    J. E. Wampler and R. J. DeSa, Appl. Spectrosc. 25, 623 (1971).CrossRefGoogle Scholar
  17. 17.
    J. E. Wampler and R. J. DeSa, Anal. Chem. 46, 563 (1974).CrossRefGoogle Scholar
  18. 18.
    The TTL Data Book for Design Engineers (Texas Instruments Inc., Dallas, Tex., 1973).Google Scholar
  19. 19.
    C. K. Turner, Science 146, 183 (1964).CrossRefGoogle Scholar
  20. 20.
    P. Rosen and G. M. Edelman, Rev. Sci. Instrum. 36, 809 (1965).CrossRefGoogle Scholar
  21. 21.
    T. D. S. Hamilton, J. Sci. Instrum. 43, 49 (1966).CrossRefGoogle Scholar
  22. 22.
    H. K. Howerton, in Fluorescence, G. G. Guilbault, ed. (Marcel Dekker, New York, 1967), p. 233.Google Scholar
  23. 23.
    B. Witholt and L. Brand, Rev. Sci. Instrum. 39, 1271 (1968).CrossRefGoogle Scholar
  24. 24.
    R. R. Schehl and R. Friedel, J. Phys. E 5, 1038 (1972).CrossRefGoogle Scholar
  25. 25.
    V. A. Mode, R. A. Thomas, and D. H. Sisson, Rev. Sci. Instrum. 71, 1714 (1970).CrossRefGoogle Scholar
  26. 26.
    J. F. Holland, R. E. Teets, and A. Timnick, Anal. Chem. 45, 145 (1973).CrossRefGoogle Scholar
  27. 27.
    R. J. DeSa, Anal. Biochem. 35, 293 (1970).CrossRefGoogle Scholar
  28. 28.
    R. Stair, R. G. Johnston, and E. W. Halbach, J. Res. Natl. Bur. Stand. (U.S.) 64 A, 291 (1960).CrossRefGoogle Scholar
  29. 29.
    J. Lavonel, C. Vernotte, B. Arrio, and F. Rocher, Biochimie 54, 161 (1972).CrossRefGoogle Scholar
  30. 30.
    R. H. McKay, Arch. Biochem. Biophys. 16, 438 (1969).Google Scholar
  31. 31.
    D. A. Deranleau, Anal. Biochem. 16, 438 (1966).CrossRefGoogle Scholar
  32. 32.
    G. Weber and B. Bablouzian, J. Biol. Chem. 241, 2558 (1966).Google Scholar
  33. 33.
    S. Ainsworth and E. Winter, Appl. Opt. 3, 371 (1964).CrossRefGoogle Scholar
  34. 34.
    W. A. Ayres, E. W. Small, and I. Isenberg, Anal. Biochem. 58, 361 (1974).CrossRefGoogle Scholar
  35. 35.
    J. C. Kemp, J. Opt. Soc. Amer. 59, 950 (1969).Google Scholar
  36. 36.
    J. B. Birks and I. H. Munro, Progr. React. Kinet. 4, 239 (1967).Google Scholar
  37. 37.
    R. Schuyler, I. Isenberg, and R. D. Dyson, Photochem. Photobiol. 15, 395 (1972).CrossRefGoogle Scholar
  38. 38.
    J. Jessop, R. P. Wayne, and T. J. Wayne, J. Phys. E 5, 638 (1972).CrossRefGoogle Scholar
  39. 39.
    M. C. Studer, U. P. Wild, and H. H. Günthard, J. Phys. E 3, 847 (1970).CrossRefGoogle Scholar
  40. 40.
    R. Schuyler and I. Isenberg, Rev. Sci. Instrum. 42, 813 (1971).CrossRefGoogle Scholar
  41. 41.
    C. Lewis, W. R. Ware, L. J. Doemeny, and T. L. Nemzek, Rev. Sc. Instrum. 44, 07 (1973).Google Scholar
  42. 42.
    L. Hundley, T. Coburn, E. Garwin, and L. Stryer, Rev. Sc. Instrum. 38, 488 (1967).CrossRefGoogle Scholar
  43. 43.
    W. R. Ware, in Creation and Detection of the Excited State, A. A. Lamola, ed. (Marcel Dekker, New York, 1971), Vol. 1A, p. 213.Google Scholar
  44. 44.
    L. Brand and J. R. Gohlke, J. Biol. Chem. 246, 2317 (1971).Google Scholar
  45. 45.
    J. N. Demas and G. A. Crosby, Anal. Chem. 42, 1010 (1970).CrossRefGoogle Scholar
  46. 46.
    R. D. Dyson and I. Isenberg, Biochemistry 10, 3233 (1971).CrossRefGoogle Scholar
  47. 47.
    A. Lemaitre and J.-P. Malengé, Comp. Biomed. Res. 4, 555 (1971).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • John E. Wampler
    • 1
  1. 1.Department of BiochemistryUniversity of GeorgiaAthensUSA

Personalised recommendations