Mapping the Three-Dimensional Structure of Proteins by Photochemical Techniques

  • Giulio Jori
  • John D. Spikes


At present, the X-ray diffraction technique is undoubtedly the most powerful method for determining the three-dimensional structure of macro-molecules in the crystalline state. Indeed, no other available technique can provide the thousands of parameters necessary to permit a detailed structural description. For the study of molecules in solution, however, other methods must be used. Such studies are especially important in the case of biomolecules that typically perform their functions in an aqueous environment. Many kinds of chemical and physical techniques have been devised for studying the structure and function of macrobiomolecules in solution. Each technique has certain advantages coupled with particular disadvantages with respect to the kinds of structural information desired. Even though these methods have not been entirely satisfactory, much information has been accumulated using them.


Aromatic Amino Acid Amino Acid Side Chain Flash Photolysis Nucleic Acid Basis Hydrated Electron 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Giulio Jori
    • 1
    • 2
  • John D. Spikes
    • 1
    • 2
  1. 1.The C.N.R. Center for the Biochemistry and Physiology of Hemocyanins and Other Metalloproteins, Istituto di Biologia AnimaleUniversita di PadovaPadovaItaly
  2. 2.Department of BiologyUniversity of UtahSalt Lake CityUSA

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