Fatty Acid Composition of Glycerolipids of Animal Tissues

  • Arnis Kuksis
Part of the Handbook of Lipid Research book series (HLRE, volume 1)


The most complete analyses of fatty acids have been obtained on total lipid extracts, although a prior separation into neutral and polar lipids has also been common. In many instances, the total fatty acid esters have been subjected to a preliminary segregation into subclasses based on unsaturation, molecular weight, and the presence or absence of functional groups. The final composition of the fatty acids in the neutral and polar lipids is then derived by normalization of the data. Detailed analyses of the total fatty acid composition of a lipid have proved essential for obtaining a complete account of the molecular species of various glycerolipids which otherwise might have been overlooked because of losses at intermediate stages of fractionation. Truly meaningful comparisons of the fatty acid composition of tissue lipids are obtained only by the examination of individual lipid classes and molecular species in specific cellular fractions. There has been a steady increase in these data, and those applying to the neutral acylglycerols have been extensively documented in other chapters of this volume.


Fatty Acid Composition Fatty Acid Methyl Ester Lipid Class Cholesteryl Ester Subcellular Fraction 
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  1. Ackman, R. G., 1972, The analysis of fatty acids and related materials by gas-liquid chromatography, in: Progress in the Chemistry of Fats and Other Lipids, Vol. 12 (R. T. Holman, ed.), pp. 165–284, Pergamon Press, Oxford.Google Scholar
  2. Ackman, R. G., and Burgher, R. D., 1965, Cod liver oil fatty acids as secondary reference standards in the GLC of polyunsaturated fatty acids of animal origin: Analysis of a dermal oil of the Atlantic leatherback turtle, J. Am. Oil Chem. Soc. 42:38.PubMedGoogle Scholar
  3. Ahluwalia, B., and Holman, R. T., 1969, Fatty acid composition of lipids of bull, boar, rabbit and human semen, J. Reprod. Fertil. 18:431.PubMedGoogle Scholar
  4. Ali, S. S., 1965, Fecal lipid excretion in man on controlled experimental diets, Ph.D. thesis, pp. 1-249, Queen’s University, Kingston, Canada.Google Scholar
  5. Ali, S. S., and Kuksis, A., 1967a, Excretion of phospholipids by man on fat-free diets, Can. J. Biochem. 45:689.PubMedGoogle Scholar
  6. Ali, S. S., and Kuksis, A., 1967b, Excretion of phospholipids by men on high fat diets, Can. J. Biochem. 45:703.PubMedGoogle Scholar
  7. Anderson, R. E., Bottino, N. R., and Reiser, R., 1970a, Animal endogenous triglycerides. I. Swine adipose tissue, Lipids 5:161.PubMedGoogle Scholar
  8. Anderson, R. E., Bottino, N. R., Cook, L. J., and Reiser, R., 1970b, Animal endogenous triglycerides. III. Swine, rat and chicken liver: Comparison with adipose tissue, Lipids 5:171.PubMedGoogle Scholar
  9. Ansari, M. N. A., Nicolaides, N., and Fu, H. C., 1970, Fatty acid composition of the living layer and stratum corneum of human sole skin epidermis, Lipids 5:838.PubMedGoogle Scholar
  10. Balint, J. A., Kyriakides, E. C., Spitzer, H. L., and Morrison, E. S., 1965, Lecithin fatty acid composition in bile and plasma of man, dogs, rats and oxen, J. Lipid Res. 6:96.PubMedGoogle Scholar
  11. Bligh, E. G., and Dyer, W. J., 1959, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol. 3:911.Google Scholar
  12. Bottino, N. R., Anderson, R. E., and Reiser, R., 1970, Animal endogenous triglycerides. II. Rat and chicken adipose tissue, Lipids 5:165.PubMedGoogle Scholar
  13. Breckenridge, W. C., and Kuksis, A., 1967, Molecular weight distribution of milk fat triglycerides from seven species, J. Lipid Res. 8:473.PubMedGoogle Scholar
  14. Breckenridge, W. C., Gombos, G., and Morgan, I. G., 1971, The docosahexaenoic acid of the phospholipids of synaptic membranes, vesicles and mitochondria, Brain Res. 33:581.Google Scholar
  15. Bruce, A., 1974, Skeletal muscle lipids. III. Changes in fatty acid composition of individual phosphoglycerides in man from fetal to middle age, J. Lipid Res. 15:109.PubMedGoogle Scholar
  16. Bruce, A., and Svennerholm, L., 1971, Skeletal muscle lipids. I. Changes in fatty acid composition of lecithin in man during growth, Biochim. Biophys. Acta 239:393.PubMedGoogle Scholar
  17. Carroll, K. K., 1965, Dietary fat and the fatty acid composition of tissue lipids, J. Am. Oil Chem. Soc. 42:516.PubMedGoogle Scholar
  18. Chin, H. P., 1970, Sphingomyelin fatty acid composition in human twins: The effects of genotype, age and sex, Biochim. Biophys. Acta 218:407.PubMedGoogle Scholar
  19. Christie, W. W., 1972. The preparation of alkyl esters from fatty acids and lipids, in: Topics in Lipid Chemistry, Vol. 3 (F. D. Gunstone, ed.), pp. 171–197, Wiley, New York.Google Scholar
  20. Christie, W. W., 1973, The structures of bile phosphatidylcholines, Biochim. Biophys. Acta 316:204.PubMedGoogle Scholar
  21. Christie, W. W., and Moore, J. H., 1970, A comparison of the structures of triglycerides from various pig tissues, Biochim. Biophys. Acta 210:46.PubMedGoogle Scholar
  22. Christie, W. W., Moore, J. H., Lorimer, A. R., and Lawrie, T. D. V., 1971, The structures of triglycerides from atherosclerotic plaques and other human tissues, Lipids 6:854.PubMedGoogle Scholar
  23. Clements, J., 1971, Comparative lipid chemistry of lungs, Arch. Intern. Med. 127:387.PubMedGoogle Scholar
  24. Cmelik, S., and Fonseca, E., 1974, Composition of mitochondrial lipids from rat adrenal glands, Hoppe-Seyler’s Z. Physiol. Chem. 355:19.PubMedGoogle Scholar
  25. Cmelik, S., and Ley, H., 1974, Fatty acid composition of some cellular and subcellular elements of the elephant adrenal gland, Hoppe-Seyler’s Z. Physiol. Chem. 355:797.PubMedGoogle Scholar
  26. Cmelik, S. H. W., and Ley, H., 1975, Distribution of cholesteryl esters and other lipids in subcellular fractions of the adrenal gland of the pig, Lipids 10:707.PubMedGoogle Scholar
  27. Colbeau, A., Nachbaur, J., and Vignais, P. M., 1971, Enzymic characterization and lipid composition of rat liver subcellular membranes, Biochim. Biophys. Acta 249:462.PubMedGoogle Scholar
  28. Comai, K., Farber, S. J., and Paulsrud, J. R., 1975, Analysis of renal medullary lipid droplets from normal, hydronephrotic and indomethacin treated rabbits, Lipids 10:555.PubMedGoogle Scholar
  29. Coniglio, J. G., Grogan, W. M., Jr., Harris, D. G., and Fitzhugh, M. L., 1975, Lipid and fatty acid composition of testes of quaking mice, Lipids 10:109.PubMedGoogle Scholar
  30. Connellan, J. M., and Masters, C. J., 1965, Fatty acid components of rat tissue lipids, Biochem. J. 94:81.PubMedGoogle Scholar
  31. Davis, J. T., Bridges, R. B., and Coniglio, J. G., 1966, Changes in lipid composition of the maturing rat testis, Biochem. J. 98:342.PubMedGoogle Scholar
  32. Downing, D. T., and Sharaf, D. M., 1976, Skin surface lipids of the guinea pig, Biochim. Biophys. Acta 431:378.PubMedGoogle Scholar
  33. Egwim, P. O., and Kummerow, F. A., 1972, Influence of dietary fat on the concentration of long-chain unsaturated fatty acid families in rat tissues, J. Lipid Res. 13:500.PubMedGoogle Scholar
  34. Ferber, E., De Pasquale, G. G., and Resch, K., 1975, Phospholipid metabolism of stimulated lymphocytes: Composition of phospholipid fatty acids, Biochim. Biophys. Acta 398:364.PubMedGoogle Scholar
  35. Fleischer, S., and Rouser, G., 1965, Lipids of subcellular particles, J. Am. Oil Chem. Soc. 42:588.PubMedGoogle Scholar
  36. Fleischer, S., Rouser, G., Fleischer, B., Casu, A., and Kritchevsky, G., 1967, Lipid composition of mitochondria from bovine heart, liver and kidney, J. Lipid Res. 8:170.PubMedGoogle Scholar
  37. Fletcher, R. F., 1972, Lipids of human myocardium, Lipids 7:728.PubMedGoogle Scholar
  38. Folch, J., Lees, M., and Stanley, G. H. S., 1957, A simple method for the isolation and purification of total lipids from animal tissues, J. Biol. Chem. 266:497.Google Scholar
  39. Glass, R. L., 1971, Alcoholysis, saponification and preparation of fatty acid methyl esters, Lipids 6:919.Google Scholar
  40. Gloster, J., and Harris, P., 1969, The lipid composition of mitochondrial and microsomal fractions of human myocardial homogenates, Cardiovasc. Res. 3:45.PubMedGoogle Scholar
  41. Gold, M., 1968, Triglyceride subclasses of various dog adipose tissue sites, Lipids 3:539.PubMedGoogle Scholar
  42. Golovnya, R. V., Uralets, V. P., and Kuzmenko, T. E., 1976, Characterization of fatty acid methyl esters by gas chromatography on siloxane liquid phases, J. Chromatogr. 121:118.PubMedGoogle Scholar
  43. Gray, G. M., and Yardley, H. J., 1975, Lipid compositions of cells isolated from pig, human and rat epidermis, J. Lipid Res. 16:434.PubMedGoogle Scholar
  44. Grigor, M. R., and Duncley, G. G., 1973, Origin of the high saturated fatty acid content of rat fecal lipids, Lipids 8:53.PubMedGoogle Scholar
  45. Grigor, M. R., Duncley, G. G., and Purves, H. D., 1970, The branched-chain fatty acids of rat fecal lipids: The contribution of the intestinal microorganisms, Biochim. Biophys. Acta 218:400.PubMedGoogle Scholar
  46. Grigor, M. R., Moehl, A., and Snyder, F., 1972, Occurrence of ethanolamine and choline-containing plasmalogens in adipose tissue, Lipids 7:766.PubMedGoogle Scholar
  47. Grogan, W. M., Jr., Coniglio, J. G., and Rhamy, R. K., 1973, Identification of some polyenoic acids isolated from human testicular tissue, Lipids 8:480.PubMedGoogle Scholar
  48. Grossi, E., Paoletti, P., and Paoletti, R., 1961, A gas—liquid chromatographic analysis of fatty acid compositions of human normal and tumoral nervous tissue, in: Proceedings of the Fourth International Congress of Neuropathology, Vol. 1 (H. Jacon, ed.), pp. 29–35, Georg Thieme Verlag, Stuttgart.Google Scholar
  49. Guarnieri, M., and Johnson, R. M., 1970, The essential fatty acids, in: Advances in Lipid Research, Vol. 8 (D. Kritchevsky and R. Paoletti, eds.), pp. 115–174, Academic Press, New York.Google Scholar
  50. Henning, R., Kaulen, H. D., and Stoffel, W., 1970, Isolation and chemical composition of the lyso-somal and the plasma membrane of the rat liver cell, Hoppe-Seyler’s Z. Physiol. Chem. 351:1191.PubMedGoogle Scholar
  51. Hilditch, T. P., and Williams, P. N., 1964, The Chemical Constitution of Natural Fats, 4th ed., Chapman and Hall, London.Google Scholar
  52. Hoffman, A. G. D., and Kuksis, A., 1977, Comparison of de novo synthesis of fatty acids between villus and crypt cells isolated from rat small intestine, Proc. Can. Fed. Biol. Soc. 20:21.Google Scholar
  53. Holman, R. T., and Hofstetter, H. H., 1965, The fatty acid composition of the lipids from bovine and porcine reproductive tissues, J. Am. Oil Chem. Soc. 42:540.PubMedGoogle Scholar
  54. Howard, B. V., Butler, J. D., and Bailey, J. M., 1973, Lipid Metabolism in Normal and Tumour Cells in Culture (R. Wood, ed.), pp. 200–214, American Oil Chemists’ Society Press, Champaign, Ill.Google Scholar
  55. Huang, T. C., and Kuksis, A., 1967, A comparative study of the lipids of globule membrane and fat core of the milk serum of cows, Lipids 2:453.PubMedGoogle Scholar
  56. Huet, C., Levy, M., and Pascaud, M., 1968, Spécificité de constitution en acides gras des phospholipides des membranes mitochondriales, Biochim. Biophys. Acta 150:521.PubMedGoogle Scholar
  57. Illingworth, D. R., and Portman, O. W., 1972, Independence of phospholipid and protein exchange between plasma lipoproteins in vivo and in vitro, Biochim. Biophys. Acta 280:281.PubMedGoogle Scholar
  58. Imaizumi, K., and Sugano, M., 1975, Glycerolipid metabolism in cellular and subcellular fractions of fasted rat liver, J. Nutr. Sci. Vitaminol. 21:223.PubMedGoogle Scholar
  59. Insull, W., Jr., Hirsch, J., James, T., and Ahrens, E. H., Jr., 1959, The fatty acids of human milk. II. Alterations produced by manipulation of caloric balance and exchange of dietary fats, J. Clin. Invest. 38:443.PubMedGoogle Scholar
  60. Jaeger, H., Kloer, H. U., and Ditschuneit, H., 1976, Automated glass capillary gas—liquid chromatography of fatty acid methyl esters with reference to cis- and trans-isomers, J. Lipid Res. 17:185.PubMedGoogle Scholar
  61. Jain, Y. C., and Anand, S. R., 1976, Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid, J. Reprod. Fertil. 47:261.PubMedGoogle Scholar
  62. James, A. T., Webb, J. P. W., and Kelloch, T. D., 1961, The occurrence of unusual fatty acids in fecal lipids from human beings with normal and abnormal fat absorption, Biochem. J. 78:333.PubMedGoogle Scholar
  63. Kang, K. W., Taylor, G. E., Geves, J. H., Staley, H. L., and Christian, J. C., 1971, Genetic variability of human plasma and erythrocyte lipids, Lipids 6:595.PubMedGoogle Scholar
  64. Keenan, T. W., and Morre, D. J., 1970, Phospholipid class of fatty acid composition of golgi apparatus isolated from rat liver and comparison with other cell fractions, Biochemistry 9:19.PubMedGoogle Scholar
  65. Kuksis, A., 1966, Quantitative lipid analysis by combined thin-layer and gas—liquid chromatographic systems, in: Chromatographic Reviews, Vol. 8 (M. Lederer, ed.), pp. 172–207, Elsevier, Amsterdam.Google Scholar
  66. Kuksis, A., 1971, Progress in the analysis of lipids. IX. Gas chromatography, Part 1, Fette Seifen Anstrichm. 71:130.Google Scholar
  67. Kuksis, A., 1972, Newer developments in determination of structure of glycerides and phosphoglycerides, in: Progress in the Chemistry of Fats and other Lipids, Vol. 12 (R. T. Holman, ed.), pp. 1–163, Pergamon Press, Oxford.Google Scholar
  68. Kuksis, A., 1977, Routine chromatography of simple lipids and their constituents, J. Chromatogr. Biomed. Appl. 143:3.Google Scholar
  69. Lagrou, A., Kierick, W., Christophe, A., and Verdonk, G., 1974, Lipid composition of normal and hypertrophic bovine thyroids, Lipids 9:870.PubMedGoogle Scholar
  70. Leikola, E., Nieminen, E., and Salomaa, E., 1965, Occurrence of methyl esters in the pancreas, J. Lipid Res. 6:490.PubMedGoogle Scholar
  71. Levis, G. M., Karli, J. N., and Malamos, B., 1972, The phospholipids of the thyroid gland, Clin. Chim. Acta 41:335.PubMedGoogle Scholar
  72. Lin, S. N., Pfaffenberger, C. D., and Horning, E. C., 1975, Thermostable glass open tubular capillary columns with a polar liquid phase for gas chromatography, J. Chromatogr. 104:319.Google Scholar
  73. Lipshaw, L. A., and Foa, P. P., 1974, The composition and possible physiological role of the thyroid lipids, in: Advances in Lipid Research, Vol. 12 (D. Kritchevsky and R. Paoletti, eds.), pp. 227–250, Academic Press, New York.Google Scholar
  74. Lough, A. K., and Garton, G. A., 1968, The lipids of human pancreas with special reference to the presence of fatty acid methyl esters, Lipids 3:321.PubMedGoogle Scholar
  75. Lough, A. K., Felinski, L., and Gordon, G. A., 1962, The production of methyl esters of fatty acids as artifacts during the extraction or storage of tissue lipids in the presence of methanol, J. Lipid Res. 3:478.Google Scholar
  76. Marai, L., and Kuksis, A., 1973, Comparative study of molecular species of glycerolipids in sarco-tubular membranes of skeletal muscle of rabbit, rat, chicken, and man, Can. J. Biochem. 51:1365.PubMedGoogle Scholar
  77. Margolis, S., 1969, Structure of very low and low density lipoproteins, in: Structure and Function of Lipoproteins in Living Systems (E. Tria and A. M. Scanu, eds.), pp. 369–424, Academic Press, New York.Google Scholar
  78. Martonosi, A., 1972, Biochemical and clinical aspects of sarcoplasmic reticulum function, in: Current Topics in Membranes and Transport, Vol. 3 (F. Bronner and A. Kleinzeller, eds.), pp. 83–197, Academic Press, New York.Google Scholar
  79. Meldolesi, J., Jamieson, J. D., and Palade, G. E., 1971, Cellular membranes in the pancreas of the guinea pig. II. Lipids, J. Cell Biol. 49:130.PubMedGoogle Scholar
  80. Mills, S. C., Cook, L. J., Scott, T. W., and Nestel, P. J., 1976, Effect of dietary fat supplementation on the composition and positional distribution of fatty acids in ruminant and porcine glycerides, Lipids 11:49.PubMedGoogle Scholar
  81. Montfort, A., Van Golde, L. M. G., and Van Deenen, L. L. M., 1971, Molecular species of lecithins from various animal tissues, Biochim. Biophys. Acta 231:335.Google Scholar
  82. Mookerjea, S., Park, C. E., and Kuksis, A., 1975, Lipid profiles of plasma lipoproteins of fasted and fed normal and choline-deficient rats, Lipids 10:374.PubMedGoogle Scholar
  83. Morrison, W. R., 1970, Milk lipids, in: Topics in Lipid Chemistry, Vol. 1 (F. D. Gunstone, ed.), pp. 51–106, Logos Press, London.Google Scholar
  84. Morrison, W. R., and Smith, L. M., 1964, Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron trifluoride-methanol, J. Lipid Res. 5:600.PubMedGoogle Scholar
  85. Nakamura, M., Jensen, B., and Privett, O. S., 1968, Effect of hypohysectomy on the fatty acids and lipid classes of rat testes, Endocrinology 82:137.PubMedGoogle Scholar
  86. Nazir, D. J., Alcaraz, A. P., and Nair, P. P., 1967a, Lipids of subcellular particles from bovine heart muscle. I. Fatty acids of neutral lipids, Can. J. Biochem. 45:1725.PubMedGoogle Scholar
  87. Nazir, D. J., Alcaraz, A. P., and Nair, P. P., 1967b, Lipids of subcellular particles from bovine heart muscle. II. Fatty acids of phospholipids, Can. J. Biochem. 45:1739.PubMedGoogle Scholar
  88. Nelson, G. J., 1972a, Quantitative analysis of blood lipids, in: Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism (G. J. Nelson, ed.), pp. 25–73, Interscience, New York.Google Scholar
  89. Nelson, G. J., 1972b, Lipid composition and metabolism of erythrocytes, in: Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism (G. J. Nelson, ed.), pp. 317–386, Interscience, New York.Google Scholar
  90. Nelson, G. J., 1975, Fractionation of phospholipids, in: Analysis of Lipids and Lipoproteins (E. G. Perkins, ed.), pp. 63–89, American Oil Chemists’ Society, Champaign, Ill.Google Scholar
  91. Nicolaides, N., and Ansari, M. N. A., 1969, The dienoic fatty acids of human skin surface lipid, Lipids 4:79.Google Scholar
  92. Nicolaides, N., Apon, J. M. B., and Wong, D. H., 1976, Further studies of the saturated methyl branched fatty acids of vernix caseosa lipid, Lipids 11:781.PubMedGoogle Scholar
  93. Nikkari, T., 1965, Composition and secretion of the skin surface lipids of the rat: Effects of dietary lipids and hormones, Scand. J. Clin. Lab. Invest. Suppl. 85:1.Google Scholar
  94. Nikkari, T., 1974, Comparative chemistry of sebum, J. Invest. Dermatol. 62:257.PubMedGoogle Scholar
  95. Odutuga, A. A., Carey, E. M., and Prout, R. E. S., 1973, Changes in the lipid and fatty acid composition of developing rabbit brain, Biochim. Biophys. Acta 316:115.PubMedGoogle Scholar
  96. Ottenstein, D. M., Bartley, D. A., and Supina, W. R., 1976, Gas chromatographic separation of cis-trans isomers: Methyl oleate/methyl elaidate, J. Chromatogr. 119:401.PubMedGoogle Scholar
  97. Parkes, J. G., and Thompson, W., 1970, The composition of phospholipids in outer and inner mitochondrial membranes from guinea pig liver, Biochim. Biophys. Acta 196:162.PubMedGoogle Scholar
  98. Poulos, A., Dari-Bennett, A., and White, I. G., 1973, The phospholipid-bound fatty acids and aldehydes of mammalian spermatozoa, Comp. Biochem. Physiol. 46B:541.Google Scholar
  99. Privett, O. S., Dougherty, K. A., and Castell, J. D., 1971, Quantitative analysis of lipid classes, Am. J. Clin. Nutr. 24:1265.PubMedGoogle Scholar
  100. Prottey, C., and Hawthorne, J. N., 1966, The lipids of mammalian pancreas, Biochem. J. 101:191.PubMedGoogle Scholar
  101. Ray, T. K., Skipski, V. P., Barclay, M., Essner, E., and Archibald, F. M., 1969, Lipid composition of rat liver plasma membranes, J. Biol. Chem. 244:5528.PubMedGoogle Scholar
  102. Richardson, T., Tappel, A. L., and Gruger, E. R., 1961, Essential fatty acids in mitochondria, Arch. Biochem. Biophys. 94:1.PubMedGoogle Scholar
  103. Rogers, C. G., 1974, Fatty acids and phospholipids of adult and newborn rat hearts and of cultured, beating neonatal rat myocardial cells, Lipids 9:541.PubMedGoogle Scholar
  104. Rouser, G., Kritchevsky, G., Yamamoto, A., Simon, G., Galli, C., and Bauman, A. J., 1969a, Diethylaminoethyl and triethylaminoethyl cellulose column chromatographic procedures for phospholipids, glycolipids and pigments, in: Methods in Enzymology, Vol. 14 (J. M. Lowestein, ed.), pp. 272–317, Academic Press, New YorkGoogle Scholar
  105. Rouser, G., Simon, G., and Kritchevsky, G., 1969b, Species variations in phospholipid class distribution of organs. I. Kidney, liver and spleen, Lipids 4:599.PubMedGoogle Scholar
  106. Rouser, G., Kritchevsky, G., and Yamamoto, A., 1976, Column chromatographic and associated procedures for separation and determination of Phosphatides and glycolipids, in: Lipid Chromatographic Analysis, Vol. 3 (G. V. Marinetti, ed.), 2nd ed., pp. 713–776, Marcel Dekker, New York.Google Scholar
  107. Scott, T. W., and Setchell, B. P., 1968, Lipid metabolism in the testes of the ram, Biochem. J. 107:273.PubMedGoogle Scholar
  108. Scott, T. W., Setchell, B. P., and Bassett, J. M., 1967, Characterization and metabolism of ovine fetal lipids, Biochem. J. 104:1040.PubMedGoogle Scholar
  109. Scott, T. W., Hansel, W., and Donaldson, L. E., 1968, Metabolism of phospholipids and the characterization of fatty acids in bovine corpus luteum, Biochem. J. 108:317.PubMedGoogle Scholar
  110. Sheppard, A. J., and Iverson, J. L., 1975, Esterification of fatty acids for gas—liquid chromatographic analysis, J. Chromatogr. Sci. 13:448.Google Scholar
  111. Siakotos, A. N., and Rouser, G., 1965, Analytical separation of non-lipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography, J. Am. Oil. Chem. Soc. 42:913.PubMedGoogle Scholar
  112. Silbert, D. F., 1975, Genetic modification of membrane lipid, Annu. Rev. Biochem. 44:315.PubMedGoogle Scholar
  113. Silbert, D. F., Cronan, J. E., Jr., Beacham, I. R., and Harder, M. E., 1974, Genetic engineering of membrane lipid, Fed. Proc. 33:1725.PubMedGoogle Scholar
  114. Skipski, V. P., 1972, Lipid composition of lipoproteins in normal and disease states, in: Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism (G. J. Nelson, ed.), pp. 471–584, Interscience, New York.Google Scholar
  115. Skipski, V. P., and Barclay, M., 1969, Thin-layer chromatography of lipids, in: Methods in Enzymology, Vol. 14 (J. M. Lowestein, ed.), pp. 530–612, Academic Press, New York.Google Scholar
  116. Stoffel, W., and Schiefer, H. G., 1968, Biosynthesis and composition of Phosphatides in outer and inner mitochondrial membranes, Hoppe-Seyler’s Z. Physiol. Chem. 349:1017.PubMedGoogle Scholar
  117. Sun, G. Y., 1973, Phospholipids and acyl groups in subcellular fractions from human cerebral cortex, J. Lipid Res. 14:656.PubMedGoogle Scholar
  118. Sun, G. Y., and Leung, B. S., 1974, Phospholipids and acyl groups of subcellular membrane fractions from human intracranial tumors, J. Lipid Res. 15:423.PubMedGoogle Scholar
  119. Svennerholm. L., 1968, Distribution and fatty acid composition of phosphoglycerides in normal human brain, J. Lipid Res. 9:570.PubMedGoogle Scholar
  120. Swell, L., Enteman, C., Leong, G. F., and Holloway, R. J., 1968, Bile acids and lipid metabolism. IV. Influence of bile acids on biliary and liver organelle phospholipids and cholesterol, Am. J. Physiol. 215:1390.PubMedGoogle Scholar
  121. Takayasu, K., Okuda, K., and Yoshikawa, I., 1970, Fatty acid composition of human and rat adrenal lipids: Occurrence of ω6 docosatrienoic acid in human adrenal cholesterol ester, Lipids 5:743.PubMedGoogle Scholar
  122. Tsao, S. S., and Cornatzer, W. E., 1967, Chemical composition of subcellular particles from cultured cells of human tissue, Lipids 2:41.PubMedGoogle Scholar
  123. Van Golde, L. M. G., Pieterson, W. A., and Van Deenen, L. L. M., 1968, Alterations in the molecular species of rat liver lecithin by corn oil feeding to essential fatty acid deficient rat as a function of time, Biochim. Biophys. Acta 157:84.Google Scholar
  124. Van Hoeven, R. P., Emmelot, P., Krol, J. H., and Oomen-Meulemans, E. P. M., 1975, Studies on plasma membranes. XXII. Fatty acid profiles of lipid classes in plasma membranes of rat and mouse livers and hepatomas, Biochim. Biophys. Acta 380:1.PubMedGoogle Scholar
  125. Veerkamp, J. H., Mulder, I., and Van Deenen, L. L. M., 1962, Comparison of the fatty acid composition of lipids from different animal tissues including some tumours, Biochim. Biophys. Acta 57:299.PubMedGoogle Scholar
  126. Vilkki, P., and Jaakonmaki, I., 1966, Role of fatty acids in iodide complexing lecithin, Endocrinology 78:453.PubMedGoogle Scholar
  127. Wells, M. A., and Dittmer, J. C., 1963, The use of Sephadex for the removal of non-lipid contaminants from lipid extracts, Biochemistry 2:1259.PubMedGoogle Scholar
  128. Wheeldon, L. W., Schumert, Z., and Turner, D. A., 1965, Lipid composition of heart muscle homogenate, J. Lipid Res. 6:481.PubMedGoogle Scholar
  129. White, D. A., 1973, The phospholipid composition of mammalian tissues, in: Form and Function of Phospholipids (G. B. Ansell, R. M. C. Dawson, and J. N. Hawthorne, eds.), 2nd ed., pp. 441–482, Elsevier, Amsterdam.Google Scholar
  130. Wilkinson, D. I., 1970, Positional isomers of monoene and diene fatty acids of human skin epidermal cells, Arch. Biochem. Biophys. 136:368.PubMedGoogle Scholar
  131. Wood, R., 1970, Plasma membranes: Structural analyses of neutral lipids and phospholipids of rat liver, Arch. Biochem. Biophys. 141:174.PubMedGoogle Scholar
  132. Wood, R., 1973, Tumour lipids: Structural and metabolism studies of Ehrlich ascites cells, in: Tumour Lipids: Biochemistry and Metabolism (R. Wood, ed.), pp. 139–182, American Oil Chemists’ Society Press, Champaign, Ill.Google Scholar
  133. Wood, R., 1976, Oleic and vaccenic acid levels in lipid classes of tumours, Lipids 11:578.PubMedGoogle Scholar
  134. Zilversmit, D. B., 1965, The composition and structure of lymph chylomicrons in dog, rat and man, J. Clin. Invest. 44:1610.PubMedGoogle Scholar
  135. Zilversmit, D. B., 1969, Chylomicrons, in: Structural and Functional Aspects of Lipoproteins in Living Systems (E. Tria and A. M. Scanu, eds.), pp. 329–368, Academic Press, New York.Google Scholar
  136. Zilversmit, D. B., 1971, Stimulation of phospholipid exchange between mitochondria and artificially prepared phospholipid aggregates by a soluble fraction from liver, J. Biol. Chem. 246:2645.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Arnis Kuksis
    • 1
  1. 1.Banting and Best Department of Medical ResearchUniversity of TorontoTorontoCanada

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