Killing of Nucleated Cells by Antibody and Complement
The ability of antibody-antigen complexes to fix complement (C) has been known since the end of the last century. Up to and including the recent past, complement has been used mainly as a very sensitive indicator to detect the interaction of antibody with antigen. With the development of refined immunochemical methods it has been possible to analyze what complement is and to study its interaction with antigen-antibody complexes and with the surface of the cell (Müller-Eberhard, 1968; Rapp and Borsos, 1970; Humphrey and Dourmashkin, 1965, 1969).
KeywordsNucleate Cell Sheep Erythrocyte Antigen Density Forssman Antibody Immune Gamma Globulin
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- Baker, A. R., Borsos, T., Colten, H. R., 1971, Cytotoxic action of antiserum and complement. Quantitation with a colony inhibition method, Immunology 101: 33–43.Google Scholar
- Borsos, T., 1971, Immunoglobulin classes and complement-fixing activity, in: Progress in Immunology, pp. 841–848, Academic Press, New York.Google Scholar
- Ellem, K. A. O., 1957, Studies on the mechanism of the cytotoxic action of antisera, Aust. J. Sci. 20: 116–117.Google Scholar
- Gorer, P. A., O’Gorman, P., 1956, The cytotoxic activity of isoantibodies in mice, Transplant. Bull. 3: 142–143.Google Scholar
- Kabat, E. A., Mayer, M. M., 1961, Experimental Immunochemistry, p. 176, Charles C. Thomas, Springfield, Illinois.Google Scholar
- Levy, N. L., Amos, D. ?., Solovieff, G. V., dos Reis, A. P., 1972, In vitro methods for assessment of antibody mediated tumor immunity, Nat. Cancer Inst. Monogr. 35:5–11.Google Scholar
- Mayer, M. M., 1961, Development of the one-hit theory of immune hemolysis, in: Immunochemical Approaches to Problems in Microbiology ( M. Heidelberger and O. J. Plescia, eds.), p. 268, Rutgers University Press, New Brunswick, New Jersey.Google Scholar
- Pellegrino, M. A., Ferrone, S., Cooper, N. R., Dierich, M. P., Reisfeld, R. A., 1974, Variation in susceptibility of a human lymphoid cell line to immune lysis during the cell cycle. Lack of correlation with antigen density and complement binding, J. Exp. Med. 140: 578–590.PubMedCrossRefGoogle Scholar
- Rapp, H. J., Borsos, T., 1970, Molecular Basis of Complement Action, Appleton-Century-Crofts, New York.Google Scholar
- Shin, H. S., Mayer, M. M., 1968, The third component of guinea pig complement system. II. Kinetic studies of the reaction of EAC′42a with guinea pig C3. Enzymatic Nature of C′3 consumption, multiphasic character of fixation, and hemolytic titration of C′3, Biochemistry 7: 2997–3002.PubMedCrossRefGoogle Scholar