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The Preparation and Analysis of Myelin from Small Quantities of Central Nervous Tissue: Regional Studies of the Quaking Mouse

  • G. E. Fagg
  • T. V. Waehneldt
  • V. Neuhoff
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 100)

Summary

Myelin of considerable purity may be isolated from small (minimum 1 mg wet weight) samples of central nervous tissue, using a 4-step centrifugation procedure. The separation of myelin proteins by micro-linear gradient polyacrylamide gel electrophoresis yields similar results to those obtained by macro-scale (homogeneous) gel systems. These techniques have been employed for a preliminary study of the regional composition of myelin fractions from the Quaking mouse.

Keywords

Protein Composition Myelin Protein Central Nervous System Region Central Nervous Tissue Small Tissue Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • G. E. Fagg
    • 1
  • T. V. Waehneldt
    • 1
  • V. Neuhoff
    • 1
  1. 1.Forschungsstelle NeurochemieMax-Planck-Institut für Experimentelle MedizinGöttingenGermany

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