Advertisement

Studies on Myelin Proteins in Human Peripheral Nerve

  • Keiichi Uyemura
  • Masaru Suzuki
  • Kunio Kitamura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 100)

Summary

The myelin fraction from human peripheral nerve was prepared. Two basic protein fractions (BF-P2 and PB) were isolated from acid extracts of the myelin fraction and three glycoproteins(BR-PO, PASII and Y protein) were purified from its acid-insoluble residue. In biochemical analysis the human BF-P2 protein (M.W.13,000) showed similar but not identical properties to bovine BF-P2 protein. The PB fraction was suggested to include the encephalitogenic CNS-BP (M.W.18,000) and another, new protein of similar molecular weight.

Both the human BR-PO protein (M.W.28,000) and PASII protein (M.W.13,000) showed similar biochemical properties to the corresponding myelin proteins of bovine peripheral nerve, while they both are clearly different from other myelin proteins. Close relationship between the BR-PO protein and the Y protein (M.W.22,000) was suggested by amino acid analysis.

Injection of the myelin fraction of bovine peripheral nerve with the complete adjuvant produced EAN while the CNS-BP induced EAE in laboratory animals. However, all three purified proteins. BF-P2, BR-PO and PASII, from bovine peripheral nerve myelin were inactive in inducing demyelinating diseases.

Keywords

Amino Acid Composition Myelin Basic Protein Basic Protein Demyelinating Disease Myelin Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviated Heading

CNS

Central nervous system

PNS

Peripheral nervous system

CNS-BP

Encephalitogenic basic protein (M.W.18,000) in CNS myelin

FLPL

Folch-Lees type proteolipid in CNS myelin

BF-P2

protein — Basic protein (M.W.13,000) in PNS myelin

BR-PO

protein — Glycoprotein (M.W.28,000) in PNS myelin

PASII

Protein — Glycoprotein (M.W.13,000) in PNS myelin

Y

Protein — Glycoprotein (M.W.22,000) in PNS myelin

SDS

Sodium dodecyl sulfate

PAS

Periodic acid-Shiff

EAN

Experimental allergic neuritis

EAE

Experimental allergic encephalomyelitis

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ansari, K.A. and Komanduri, V., Human myelin basic proteins: A comparison of proteins from six different anatomic sites, Neurology 24 (1974) 94–98.PubMedCrossRefGoogle Scholar
  2. 2.
    Brostoff, S., Burnett, P., Lampert, P. and Eylar, E.H., Isolation and characterization of a protein from sciatic nerve myelin responsible for experimental allergic neuritis, Nature New Biol. 235 (1972) 210–212.PubMedGoogle Scholar
  3. 3.
    Brostoff, S.W. and Eylar, E.H., The proposed amino acid sequence of the P1 protein of rabbit sciatic nerve myelin, Arch. Biochem. Biophys. 153 (1972) 590–598.PubMedCrossRefGoogle Scholar
  4. 4.
    Brostoff, S.W., Karkhanis, Y.D., Carlo, D.J., Reuter, W. and Eylar, E.H., Isolation and partial characterization of the major proteins of rabbit sciatic nerve myelin, Brain Res. 86 (1975) 449–458.CrossRefGoogle Scholar
  5. 5.
    Brostoff, S.W., Sacks, H., Dal Canto, M., Johnson, A.B., Raine, C.S. and Wisniewski, H., The P2 protein of bovine root myelin: Isolation and some chemical and immunological properties, J. Neurochem. 24 (1975) 1037–1043.CrossRefGoogle Scholar
  6. 6.
    Brostoff, S.W., Sacks, H. and Dipaola, C., The P2 protein of bovine root myelin: Partial chemical characterization, J. Neurochem. 24 (1975) 289–294.PubMedCrossRefGoogle Scholar
  7. 7.
    Capaldi, R.A. and Vanderkooi, G., The low polarity of many membrane proteins, Proc. Nat. Acad. Sci. U.S.A. 69 (1972) 930–932.CrossRefGoogle Scholar
  8. 8.
    Carnegie, P.R., Amino acid sequence of the encephalitogenic basic protein of human myelin, Biochem. J. 123 (1971) 57–67.PubMedGoogle Scholar
  9. 9.
    Carnegie, P.R. and Dunkley, P.R., Basic proteins of central and peripheral nervous system, in Advances in Neurochemistry Vol. 1 (B.W. Agranoff and M.H. Aprison, eds.) (1975) pp. 96–135.Google Scholar
  10. 10.
    Deibler, G.E., Kies, M.W. and Uyemura, K., Structural studies on basic protein isolated from peripheral nerve myelin, Trans. Am. Soc. Neurochem. 6 (1975) 85.Google Scholar
  11. 11.
    Everly, J.L., Brady, R.O. and Quarles, R.H., Evidence that the major protein in rat sciatic nerve myelin is a glycoprotein, J. Neurochem. 21 (1973) 329–334.PubMedCrossRefGoogle Scholar
  12. 12.
    Fairbanks, G., Steck, T.L. and Wallach, D.F.H., Electrophoretic analysis of the major polypeptides of human erythrocyte membrane, Biochemistry 10 (1971) 2606–2617.PubMedCrossRefGoogle Scholar
  13. 13.
    Folch-Pi, J. and Lees, M., Proteolipides, a new type of tissue lipoproteins, J. Biol. Chem. 191 (1951) 807–817.Google Scholar
  14. 14.
    Greenfield, G., Brostoff, S., Eylar, E.H. and Morell, P., Protein composition of myelin of the peripheral nervous system, J. Neurochem. 20 (1973) 1207–1216.PubMedCrossRefGoogle Scholar
  15. 15.
    Kies, M.W., Deibler, D.E., Kramer, A., MacPherson, C.F. and Uyemura, K., Immunological and chemical similarities between PNS and CNS basic protein, Abstr., 5th Int. Meet. Int. Soc. Neurochem. (1975) p. 419.Google Scholar
  16. 16.
    Kitamura, K., Suzuki, M. and Uyemura, K., Purification and partial characterization of two glycoproteins in bovine peripheral nerve myelin membrane, Biochim. Biophys. Acta 445 (1976) 806–816.Google Scholar
  17. 17.
    Kitamura. K., Yamanaka, T. and Uyemura, K., On basic proteins in bovine peripheral nerve myelin, Biochim. Biophys. Acta 379 (1975) 582–591.Google Scholar
  18. 18.
    Laatsch, M., Kies, M., Gordon, S. and Alvord, E., The encephalitogenic activity of myelin isolated by ultracentrifugation, J. Exp. Med. 115 (1962) 777–788.PubMedCrossRefGoogle Scholar
  19. 19.
    Luft, J.H., Improvements in epoxyresin embedding methods, J. Biophys. Biochem. Cytol. 9 (1961) 409–419.PubMedCrossRefGoogle Scholar
  20. 20.
    Mehl, E. and Wolfgram, E., Myelin types with different protein components in same species, J. Neurochem. 16 (1969) 1091–1097.PubMedCrossRefGoogle Scholar
  21. 21.
    Palo, J., Savolainen, H. and Haltia, M., Proteins of peripheral nerve myelin in diabetic neuropathy, J. neurol. Sci. (1972) 193–199.Google Scholar
  22. 22.
    Reisfeld, R.A., Lewis, U.J. and Williams, D.E., Disk electrophoresis of basic peptides on polyacrylamide gels, Nature 195 (1962) 281–283.PubMedCrossRefGoogle Scholar
  23. 23.
    Shapiro, A.L., Vinuela, E. and Maizel, J.V., Molecular weight estimation of polypeptide chain by electrophoresis in SDS polyacrylamide gel, Biochem. Biophys. Res. Comm. 28 (1967) 815–820.CrossRefGoogle Scholar
  24. 24.
    Singer, S.J. and Nicolson, G.L., The fluid mosaic model of the structure of cell membranes, Science 175 (1972) 720–730.PubMedCrossRefGoogle Scholar
  25. 25.
    Singh, H. and Spritz, N., Polypeptide components of myelin from rat peripheral nerve, Biochim. Biophys. Acta 351 (1974) 379–386.Google Scholar
  26. 26.
    Spritz, N., Singh, H. and Geyer, B., Myelin from human peripheral nerves, J. Clin. Invest. 52 (1973) 520–523.PubMedCrossRefGoogle Scholar
  27. 27.
    Uyemura, K., Kato-Yamanaka, T. and Kitamura, K., Distribution and optical activity of the basic protein in bovine peripheral nerve myelin, J. Neurochem. 29 (1977) 61–68.PubMedCrossRefGoogle Scholar
  28. 28.
    Uyemura, K., Kitamura, K., Ogawa, Y. and Matsuyama, H., Studies on the antigenic protein to induce experimental allergic neuritis, in The Aetiology and Pathogenesis of the Demyelinating Disease Z(H. Shiraki, T. Yonezawa and Y. Kuroiwa, eds.) (1976) Japan Science Press, pp. 181–192.Google Scholar
  29. 29.
    Uyemura, K., Kitamura, K. and Suzuki, M., Distribution and developmental changes of membrane glycoproteins in peripheral nerve myelin, Abstr., 6th Int. Meet. Int. Soc. Neurochem. Copenhagen (1977).Google Scholar
  30. 30.
    Uyemura, K., Tobari, C. and Hirano, S., Purification and properties of basic proteins in pig spinal cord and peripheral nerve, Biochim. Biophys. Acta 214 (1970) 190–197.Google Scholar
  31. 31.
    Uyemura, K., Tobari, C., Hirano, S. and Tsukada, Y., Comparative studies on the myelin proteins of bovine peripheral nerve and spinal cord, J. Neurochem. 19 (1972) 2607–2614.PubMedCrossRefGoogle Scholar
  32. 32.
    Vacher-Lepretre, M., Nicot, C., Alfsen, A., Jolles, J. and Jolles, P., Study of the apoprotein of Folch-Pi bovine proteolipid, Biochim. Biophys. Acta 420 (1976) 323–331.Google Scholar
  33. 33.
    Waksman, B.H. and Adams, R.D., Allergic neuritis: An experimental disease of rabbits induced by the injection of peripheral nervous tissue and adjuvants, J. Exp. Med. 103 (1955) 213–235.CrossRefGoogle Scholar
  34. 34.
    Wood, J.G. and Dawson, R.M.C., A major myelin glycoprotein of sciatic nerve, J. Neurochem. 21 (1973) 717–719.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Keiichi Uyemura
    • 1
  • Masaru Suzuki
    • 1
  • Kunio Kitamura
    • 1
  1. 1.Department of PhysiologySaitama Medical SchoolMoroyama, Irumagun, SaitamaJapan

Personalised recommendations