Topographic Analysis of MS and Control Brains
Autopsy specimens from five MS and six control cases were subjected to morphological and biochemical investigations including protein electrophoresis. On the basis of this study individual differences between the enzyme activities and the effect of storage must be taken into consideration when studying autopsy material. Furthermore, good morphological controls are required when biochemical changes are studied, especially in diseases like MS where local changes are found.
Biochemical and morphological studies showed that multiple protein bands, possibly GFA, may be noticed in gliotic areas. The activity of β-glucuronidase seemed to correlate with gliosis but the increase of this enzyme did not seem to be the first change in the plaque areas. It was suggested that the activation of carboxylic acid esterase preceded the activation of β-glucuronidase. The activities of lysosomal hydrolases seemed to decrease to a normal level in morphologically normal brain areas outside the plaques. In light microscopy, cells resembling plasma cells and containing Russel bodies were found.
KeywordsMultiple Sclerosis White Matter Posterior Horn Acid Proteinase Leucine Aminopeptidase
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