Abnormal Myelin Maturation In Vitro: the Role of Cerebrosides
Myelinating neonatal rat cerebellar explants were maintained for up to 130 days in vitro. Myelin fractions were extracted from explants of different ages and purified by density gradient centrifugation. The three fractions obtained were termed “light myelin”, “heavy myelin” and “membrane fraction” and were deficient in glycolipids compared to myelin fractions prepared from 15-day-old rat cerebellum. The culture myelin has an apparently normal ultra-structure but may not be as stable as myelin with a normal glycolipid composition.
KeywordsMultiple Sclerosis Membrane Fraction Myelinated Axon Myelin Membrane Chick Embryo Extract
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