Senescence pp 105-109 | Cite as

Discussion of Dr. Croce’s Paper

  • Orlando J. Miller
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 2)


Carlo Croce has presented very convincing evidence that SV40 viral expression and malignancy are dominant in mouse-human hybrid cells. He has shown that the virus integrated into chromosome 7 in three independently transformed human lines and into chromosome 17 in a fourth. Clearly there is not a single site of integration, and one wonders whether chromosome 7 (or 17, for that matter) has more than one site of SVM-0 integration. This question could be answered by mapping the integration site, much as one would map any other gene locus, by starting with cell lines in which chromosome 7 has been split into two parts by a translocation, or by inducing breaks in chromosome 7 by irradiating hybrid cells in which there is still a diploid mouse complement and the only human chromosome present is a number 7 carrying an integrated SV40 viral genome.


Hybrid Cell Murine Leukemia Virus Teratocarcinoma Cell SV40 Virus Normal Diploid Cell 
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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Orlando J. Miller
    • 1
  1. 1.College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

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