Discussion of Dr. Croce’s Paper
Carlo Croce has presented very convincing evidence that SV40 viral expression and malignancy are dominant in mouse-human hybrid cells. He has shown that the virus integrated into chromosome 7 in three independently transformed human lines and into chromosome 17 in a fourth. Clearly there is not a single site of integration, and one wonders whether chromosome 7 (or 17, for that matter) has more than one site of SVM-0 integration. This question could be answered by mapping the integration site, much as one would map any other gene locus, by starting with cell lines in which chromosome 7 has been split into two parts by a translocation, or by inducing breaks in chromosome 7 by irradiating hybrid cells in which there is still a diploid mouse complement and the only human chromosome present is a number 7 carrying an integrated SV40 viral genome.
KeywordsHybrid Cell Murine Leukemia Virus Teratocarcinoma Cell SV40 Virus Normal Diploid Cell
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