Senescence pp 87-103 | Cite as

Mapping the SV40 Integration Sites in SV40-Transformed Human Cells

  • Carlo M. Croce
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 2)


Since somatic cell hybrids between rodent cells derived from continuous cell lines and human cells lose (segregate) human chromosomes (Weiss and Green, 1967), it becomes possible to map human genes to specific human chromosomes (Ruddle, 1973) by determining whether the expression of a given human phenotype segregates concordantly with the presence of a specific human chromosome in the hybrid cells. Cells derived from different mammalian species, including man (Girardi et al., 1965), can be transformed by the small DNA tumor virus, simian virus 40 (SV40). The SV40 genome becomes covalently linked to cellular DNA in these cells (Sambrook et al., 1968), which also express a nuclear tumor (T) antigen (Black. et al., 1963), apparently coded by a viral gene (Tegtmeyer, 1974), and a tumor specific transplantation antigen on the cell surface (Habel, 1965). Fusion of T antigen-positive with T antigen-negative cells results in the production of heterokaryons which express T antigen in the nuclei derived from both parent cells (Steplewski et. al., 1968) and in the production of T antigenpositive and negative hybrid cells (Weiss, 1970).


Human Chromosome Thymidine Kinase Mouse Chromosome Simian Virus Somatic Cell Hybrid 
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  1. Black, P. H., W. P. Rowe, H. C. Turner and R. J. Huebner (1963) A specific complement fixing antigen present in SV40 tumor and transformed cells. Proc. Nat. Acad. Sci. USA 50, 1148–1156.PubMedCrossRefGoogle Scholar
  2. Caspersson, T., L. Zech, C. Johansson and E. J. Modest (1970) Identification of human chromosomes by DNA-binding fluorescent agent. Chromosome 30, 215–227.CrossRefGoogle Scholar
  3. Croce, C. M. (1977) Assignment of the integration site for simian virus 40 to chromosome 17 in GM54VA, a human cell line transformed by simian virus 40. Proc. Nat. Acad. Sci. USA, in press.Google Scholar
  4. Croce, C. M. and H. Koprowski (1974a) Concordant segregation of the expression of SV40 T antigen and human chromosome 7 in mousehuman hybrid subclones. J. Exp. Medo 139, 1350–1353.CrossRefGoogle Scholar
  5. Croce, C. M. and H. Koprowski (1974b) Somatic cell hybrids between mouse peritoneal macrophages and SV40-transformed human cells. J. Exp. Med. 140, 1221–1224.PubMedCrossRefGoogle Scholar
  6. Croce, C. M., A. J. Girardi and H. Koprowski (1973) Assignment of the T-antigen gene of simian virus 40 to human chromosome C-7. Proc. Nat. Acad. Sci. USA 70, 3617–3620.PubMedCrossRefGoogle Scholar
  7. Croce, C. M., D. Aden and H. Koprowski (1975a) Somatic cell hybrids between mouse peritoneal macrophages and simian virus-40-transformed human cells: II. Presence of human chromosome 7 carrying the simian virus 40 genome in cells of tumors induced by hybrid cells. Proc. Nat. Acad. Sci. USA 72, 1397–1400.PubMedCrossRefGoogle Scholar
  8. Croce, C. M., D. Aden and H. Koprowski (1975b) Tumorigenicity of mouse-human diploid hybrids in nude mice. Science 190, 1200–1202.PubMedCrossRefGoogle Scholar
  9. Epifanova, O. I. and V. V. Terskikh (1969) On the resting periods in the cell life cycle. Cell Tissue Kineto 2, 75–93.Google Scholar
  10. Girardi, A. J., F. Jensen and H. Koprowski (1965) SV40-induced transformation of human diploid cells: crisis and recovery. J. Cell Comp. Physoil. 65: 69–84.CrossRefGoogle Scholar
  11. Habel, K. (1965) Specific complement fixing antigens in polyoma tumors and infected cells. Virology 25, 55–61.PubMedCrossRefGoogle Scholar
  12. Harris, H., O. J. Miller, G. Klein, P. Worst and T. Tachibana (1969) Suppression of malignancy by cell fusion. Nature 223, 363–368.PubMedCrossRefGoogle Scholar
  13. Khoury, G. and C. M. Croce (1975) Quantitation of the viral DNA present in somatic cell hybrids between mouse cells and SV40-transformed human cells. Cell 6, 535–542.CrossRefGoogle Scholar
  14. Klein, G., U. Bregula, F. Wiener and H. Harris (1971) The analysis of malignancy by cell fusion. I. Hybrids between tumor cells and L cell derivatives. J. Cell Sci. 8, 659–672.PubMedGoogle Scholar
  15. Littlefield, J. W. (1964) Selection of hybrids from mating of fibroblasts in vitro and their presumed recombinant. Science 145, 709–710.PubMedCrossRefGoogle Scholar
  16. Ruddle, F. H. (1973) Linkage analysis in man by somatic cell genetics. Nature 242, 163–169.CrossRefGoogle Scholar
  17. Rygaard, J. and C. O. Paulsen (1969) Heterotransplantation of a human malignant tumor in nude mice. Acta Pathol. Microbiol. Scand. 27, 758–760.Google Scholar
  18. Sambrook, J., H. Westphal, P. R. Srinivasan and R. Dulbecco (1968) The state of viral DNA in cells transformed by SV40. Proc. Nat. Acad. Sci. USA 60, 1288–1295.PubMedCrossRefGoogle Scholar
  19. Seabright, M. (1971) A Rapid banding technique for human chromosomes. Lancet 2, 971–972.PubMedCrossRefGoogle Scholar
  20. Steplewski, Z., B. B. Knowles and H. Koprowski (1968) The mechanism of internuclear transmission of SV40-indiced complement fixation antigen in heterokaryocytes. Proc. Nato Acad. Sci. USA 59, 769–776.CrossRefGoogle Scholar
  21. Tegtmeyer, P. (1974) Altered patterns of protein synthesis in infection by SV40 mutants. Cold Spring Harbor. Symp. Quant. Biol. 39, 9–15.CrossRefGoogle Scholar
  22. Weiss, M. C. (1970) Further studies on loss of T antigen from somatic cell hybrids between mouse cells and SC40-transformed human cells. Proc. Nat. Acad. Sci. USA 66, 79–86.PubMedCrossRefGoogle Scholar
  23. Weiss, M. and H. Green (1967) Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc. Nat. Acad. Sci. USA 38, 1104–1111.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Carlo M. Croce
    • 1
  1. 1.The Wistar Institute of Anatomy and BiologyPhiladelphiaUSA

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