Fusion of Cytoplasts and Karyoplasts and How This Pertains to Research of Aging and Transformation
Before describing a method for fusion of isolated karyoplasts and cytoplasts of the human diploid cell WI-38, I would like to review some of the previous techniques and research involving cell hybridization and nuclear transfer. After this introduction I will suggest ways in which I feel the technique which I have developed in the laboratory of Dr. L. Hayflick at Stanford University can overcome many of the drawbacks found with the earlier techniques, as well as ways in which this particular approach can be utilized to study aging and malignancy.
KeywordsNuclear Transfer Binucleate Cell Population Doubling Level HeLa Nucleus Plastic Disc
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