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Senescence pp 57-72 | Cite as

Fusion of Cytoplasts and Karyoplasts and How This Pertains to Research of Aging and Transformation

  • Audrey L. Muggleton-Harris
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 2)

Abstract

Before describing a method for fusion of isolated karyoplasts and cytoplasts of the human diploid cell WI-38, I would like to review some of the previous techniques and research involving cell hybridization and nuclear transfer. After this introduction I will suggest ways in which I feel the technique which I have developed in the laboratory of Dr. L. Hayflick at Stanford University can overcome many of the drawbacks found with the earlier techniques, as well as ways in which this particular approach can be utilized to study aging and malignancy.

Keywords

Nuclear Transfer Binucleate Cell Population Doubling Level HeLa Nucleus Plastic Disc 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Klein WG, and Harris H. (1971) J Cell Sci 8:618Google Scholar
  2. 2.
    Klein WG, and Harris H. (1974) J Cell Sci 15:177PubMedGoogle Scholar
  3. 3.
    Stanbridge EJ. (1976) Nature (In press)Google Scholar
  4. 4.
    Norwood TH, Pendergrass WR, Sprague C.A, and Martin EM. (1974) Proc Nat Acad Sci USA 71,6:2231PubMedCrossRefGoogle Scholar
  5. 5.
    Rao NMV. (1975) 15th Ann Mtg, Amer Soc for Cell BiolGoogle Scholar
  6. 6.
    Wright WE, and Hayflick L. (1973) Proc Soc Exp Biol Med 144:487Google Scholar
  7. 7.
    Wright WE, and Hayflick L. (1975) Exp Cell Res 96:113PubMedCrossRefGoogle Scholar
  8. 8.
    Hammerling J. (1953) Int Rev Cytol 2:475CrossRefGoogle Scholar
  9. 9.
    Berger S, and Schweiger HG. (1975) J Cell Sci 17:517PubMedGoogle Scholar
  10. 10.
    Muggleton A, and Danielli JF. (1968) Exp Cell Res 49:116PubMedCrossRefGoogle Scholar
  11. 11.
    Muggleton-Harris AL, and Pezzella K. (1972) Exp Gerontol 7:427PubMedCrossRefGoogle Scholar
  12. 12.
    Gurdon JB, and Lasky RA. (1970) J Embryol exp Morph 24:227PubMedGoogle Scholar
  13. 13.
    McAvoy JW, and Dixon KE. (1974) J Exp Zool 189:243PubMedCrossRefGoogle Scholar
  14. 14.
    Diacumakos, EG. (1973) Proc Nat Acad Sci USA 70, 12:3382PubMedCrossRefGoogle Scholar
  15. 15.
    Muggleton-Harris AL, and Hayflick L. (1976) Exp Cell Res (Submitted for publication)Google Scholar
  16. 16.
    Poste G. (1970) Microbios 2:227Google Scholar
  17. 17.
    Poste G, Reeve P. Methods in Cell Biology (ed DM Prescott)#7, p 228. Acad Press, New York (1973)Google Scholar
  18. 18.
    Hayflick L, and Moorhead PS. (1961) Exp Cell Res 25:86CrossRefGoogle Scholar
  19. 19.
    Hayflick L. (1965) Exp Cell Res 37:614PubMedCrossRefGoogle Scholar
  20. 20.
    Hayflick L. (1976) The cellular basis for biological aging. In Handbook on the Biology of Aging (eds C Finch, L Hayflick) Van Nostrand Reinhold, New York (In press)Google Scholar
  21. 21.
    Ege T, and Ringertz NR (1975) Exp Cell Res 94:469PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Audrey L. Muggleton-Harris
    • 1
  1. 1.Department of Medical MicrobiologyStanford University School of MedicineStanfordUSA

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