Senescence in Cell Culture: An Accumulation of Errors or Terminal Differentiation?
Senescence in cell culture represents an ideal model system to examine a variety of questions related to cellular aging. The process of aging in vitro can be followed conveniently and precisely by measurement of the saturation density of the culture (1), the percentage of clones that can proliferate (2) or the percentage of cells incorporating 3H-thymidine under a given standard set of conditions (3). In addition, other physiologic changes which parallel the losses in proliferative capacity such as the increase in lysosomes and lysosomal enzyme activity (4–6) can be conveniently studied using proliferative activity as an independent measure of population age. In these cultures, most, if not all, environmental factors can be controlled. Thus, we can design experiments to determine the answer to fundamental questions at the cellular level which could not be answered easily in vivo.
KeywordsError Theory Free Radical Reaction Amino Acid Analogue Lysosomal Enzyme Activity Ideal Model System
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