Theoretical Mechanisms of in Vitro Senescence

  • F. Marott Sinex
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 2)


In this chapter we will discuss how somatic mutations might be involved in the senescence of cells in culture and in the intact organism. We will also discuss the possible role that stable phenotypic variations may play in senescence.


Somatic Mutation Chromosomal Aberration Thymidine Kinase Radiation Injury Recessive Mutation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alexander, P., and Connell, D.I., 1960. Shortening of the life span of mice by irradiation with x-rays and treatment with radiomimetic chemicals. Radiation Res. 12:38–48.PubMedCrossRefGoogle Scholar
  2. Burnet, Sir MacF., 1974. INTRINSIC MUTAGENESIS: A GENETIC APPROACH TO AGING, Wiley, New York-Toronto.CrossRefGoogle Scholar
  3. Casarett, G.W., 1964. Similarities and contrasts between radiation and time pathology. In: Advances in Gerontological Research I, B.L. Strehler, ed., pp. 109-163, Academic Press.Google Scholar
  4. Chasin, L.A. and Urlaub, G., 1975. Chromosorne-wide event accompanies the expression of recessive mutations in tetraploid cells. Science 187:1091–1093.PubMedCrossRefGoogle Scholar
  5. Christiansen, G., and Griffith, J., 1977. Salt and divalent cations affect the flexible nature of the natural beaded chromatin structure. Nucleic Acids Res. 4:1837.PubMedCrossRefGoogle Scholar
  6. Clark, A.M., and Rubin, M.A., 1961. The modification by x-irradiation of the lifespan of haploid and diploids of the wasp, Habrobracon sp. Radiation Res. 15:244.PubMedCrossRefGoogle Scholar
  7. Curtis, Jr., H., 1966. BIOLOGICAL MECHANISMS OF AGING, Thomas, Springfield.Google Scholar
  8. Curtis, J.M., and Miller, K., 1971. Chromosomal aberrations of liver cells of guinea pigs. J. Gerontol. 26:292–293.PubMedGoogle Scholar
  9. DeMars, R., 1974. Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detection. Mutation Res. 24:335–364.PubMedCrossRefGoogle Scholar
  10. Gurdon, J.B., 1973–74. Gene expression in early animal development: The study of its control by the microinjection of amphibian eggs. Harvey Lectures 69:49–69.PubMedGoogle Scholar
  11. Harris, M., 1971. Mutation rates in cells at different ploidy levels. J. Cell. Physiol. 78:177–184.PubMedCrossRefGoogle Scholar
  12. Hart, R.W. and Setlow, R.B., 1974. Correlation between deoxyribonucleic acid excision repair and life-span in a number of mammalian species. Proc. Nat. Acad. Sci. USA 71:2169–2173.PubMedCrossRefGoogle Scholar
  13. Hart, R.W. and Setlow, R.B., 1977. DNA repair in late passage human cells. Mechan. Aging and Devel. 5:67–77.CrossRefGoogle Scholar
  14. Heneen, W.K. and Nichols, W.W., 1972. Nonrandom arrangement of metaphase chromosomes in cultured cells of the Indian deer Muntiacus muntjac. Cytogenetics II:153–164.CrossRefGoogle Scholar
  15. Herrmann, R.L., Bach, M.D., Dowling, L. and Russell, A.P., 1975. Age-related changes in a spontaneous reassociating fraction of mouse DNA. Mech. Age. and Devel. 4:181–189.CrossRefGoogle Scholar
  16. Jacobs, P.A., Brunton, M., Court Brown, W.M., Doll, R. and Goldstein, H., 1963. Change of human chromosome count distributions with age: evidence for a sex difference. Nature, Lond., 197:1080.CrossRefGoogle Scholar
  17. Jacobs, P.A., Court Brown, W.M., and Doll, R., 1961. Distribution of human chromosome counts in relation to age. Nature, Lond., 191:1178.CrossRefGoogle Scholar
  18. Jarvik, L.F., Yen, F., and Moralishvili, E., 1974. Chromosome examinations in aging institutionalized women. Jrl. Gerontol. 29:269–2761Google Scholar
  19. Kihlman, B.A., 1966. ACTIONS OF CHEMICALS ON DIVIDING CELLS, Prentice Hall, Englewood-Cliffs, N.J. 260 pp.Google Scholar
  20. Kurtz, D.I., Russell, A.P., and Sinex, F.M., 1974. Multiple peaks in the derivative melting curve of chromatin from animals of varying age. Mech. Aging and Devel. 3:37–49.CrossRefGoogle Scholar
  21. Lamb, M.J. and Maynard Smith, J., 1964. Radiation and aging in insects. Exper. Gerontol. 1:11–20.CrossRefGoogle Scholar
  22. Martin, G.M., and Sprague, C.A., 1969. Parasexual cycle in cultivated human somatic cells. Science 166:761–763.PubMedCrossRefGoogle Scholar
  23. Masters, P.M., Bada, J.L. and Sigler, J.S., 1977. Aspartic acid racemization in the human lens during aging and in cataract formation. Nature 268:71–73.PubMedCrossRefGoogle Scholar
  24. Mattern, M.R. and Cerutti, P.A., 1975. Age-dependent excision repair of damaged thymine from γ-irradiated DNA by isolated nuclei from human fibroblasts. Nature 254:450–452.PubMedCrossRefGoogle Scholar
  25. Mayne, R., Vail, M., Mayne, P. and Metler, E.J., 1976. Changes in type of collagen synthesized in clones of chick chondrocytes grow and eventually lose division capacity. Proc. Nat. Acad. Sci. 73:1674–78.PubMedCrossRefGoogle Scholar
  26. Mays, L., 1977. Abstracts of 7th Annual Meeting American Aging Association.Google Scholar
  27. Mezger-Freed, L., 1972. Effect of ploidy and mutagens on bromodeoxyuridine resistance in haploid and diploid frog cells. Nature New Biol. 235:245–246.PubMedCrossRefGoogle Scholar
  28. O’Meara, A., and Herrman, R., 1927. A modified chromatin preparation displaying age related differences in salt dissociation and template ability. Biochem. Biophys. Acta., 269:419–429.Google Scholar
  29. Palmer, W.G. and Papaconstantinou, J., 1969. Aging of α-crystallins during development of the lens. Proc. Nat. Acad. Sci. 64:404–10.PubMedCrossRefGoogle Scholar
  30. Robinson, A.B., 1974. Evaluation and the distribution of glutaminyl and asparaginyl residues in proteins. Proc. Nat. Acad. Sci. 71:885–888.PubMedCrossRefGoogle Scholar
  31. Sinex, F.M., 1976. Molecular genetics of aging. In: HANDBOOK ON THE BIOLOGY OF AGING, C. Finch and L. Hayflick, eds. Van Nostrand Reinhold, New York, pp. 37–62.Google Scholar
  32. Sinex, F.M., 1974. The mutation theory of aging. In: THEORETICAL ASPECTS OF AGING, M. Rockstein, ed., pp. 23–31, Academic Press, New York.Google Scholar
  33. Sinex, F.M., 1960. Aging and the liability of irreplaceable molecules. II amide groups of collagen. Jr1. Gerontol. 15:15–18.Google Scholar
  34. Strauss, B.S., 1974. Repair of DNA in mammalian cells. Life Sciences 15:1685.PubMedCrossRefGoogle Scholar
  35. Strehler, B.L., 1959. Origin and comparison of the effects of time and high-energy radiations on living systems. The Quarterly Rev. of Biol. 34:117–142.CrossRefGoogle Scholar
  36. Szilard, L., 1959. On the nature of the aging process. Proc. Nat. Acad. Sci. 45:30–45.PubMedCrossRefGoogle Scholar
  37. Tas, S., 1976. Disulfide bonding in chromatin proteins with age and a suggested mechanism for aging and neoplasia. Exp. Gerontol. 11:17–24.PubMedCrossRefGoogle Scholar
  38. Upton, A.C., 1957. Ionizing radiation and the aging process, a review. Jr1. Gerontol. 12:306–313.Google Scholar
  39. Upton, A.C., 1977. Pathobiology. In: HANDBOOK OF THE BIOLOGY OF AGING, C. Finch and L. Hayflick, eds. Van Nostrand Reinhold, New York, pp. 513–535.Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • F. Marott Sinex
    • 1
  1. 1.Boston University School of MedicineBostonUSA

Personalised recommendations