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UV-A pp 107-139 | Cite as

Immediate and Short-Term Biologic Effects of Ultraviolet Radiation on Normal Skin

  • John A. Parrish
  • R. Rox Anderson
  • Frederick Urbach
  • Donald Pitts

Abstract

A host of biologic effects of ultraviolet exposure of normal human skin begin immediately after absorption of ultraviolet photons within the tissue. Biophysical and photochemical molecular events are followed by alterations in biochemistry and subsequent changes in cell metabolism. Electron-microscopic and histochemical studies demonstrate changes in cell structure and function occurring in minutes to hours after UV exposure. Subsequently, products of photochemistry and altered metabolism lead to cellular changes recognizable by routine light microscopy. Over hours to days, changes in blood flow, cell kinetics, and pigment production cause grossly observable changes in the whole organ.

Keywords

Human Skin Stratum Corneum Ultraviolet Radiation Exposure Dose Minimal Erythema Dose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Loomis, W. F. Rickets. Sci. Am. 223: 11–91, 1970.CrossRefGoogle Scholar
  2. 2.
    Neer, R., Davis, T., Walcott, A., Koshi, S., Schepis, P., Taylor, I., Thorington, L., and Wurtman, R. Stimulation by artificial lighting of calcium in elderly human subjects. Nature 229: 255–251, 1971.CrossRefGoogle Scholar
  3. 3.
    Reinertson, R. P., and Wheatley, V. R. Studies on the chemical composition of human epidermal lipids. J. Invest. Dermatol. 32: 49–59, 1959.Google Scholar
  4. 4.
    Holick, M. F., DeLuca, H. F., and Avioli, L. V. Isolation and identification of 25-hydroxycholecalciferol from human plasma. Arch. Intern. Med. 129: 56–61, 1972.CrossRefGoogle Scholar
  5. 5.
    Alvioli, L. V. Vitamin D, the kidney, and calcium homeostasis. Kidney Int. 2: 241–246, 1972.CrossRefGoogle Scholar
  6. 6.
    Norman, A. W. Evidence for a new kidney-produced hormone, 1,25 dihydroxycholecalciferol, the proposed biologically active form of vitamin D. Am. J. Clin. Nutr. 24: 1346–1351, 1971.Google Scholar
  7. 7.
    Logan, G., and Wilhelm, D. L. The inflammatory reaction in ultraviolet injury. Br. J. Exp. Pathol. 47: 286–299, 1966.Google Scholar
  8. 8.
    Cotran, R. S., and Pathak, M. A. The pattern of vascular leakage induced by monochromatic UV irradiation in rats, guinea pigs and hairless mice. J. Invest. Dermatol. 57: 155–164, 1968.Google Scholar
  9. 9.
    Logan, G., and Wilhelm, D. L. Ultra-violet injury as an experimental model of the ¬tory reaction. Nature 798: 968–969, 1963.CrossRefGoogle Scholar
  10. 10.
    Sim, M. F. The response of mouse skin to ultraviolet irradiation and its modification by drugs. In Proceedings of the International Symposium on Non-Steroidal Anti-Inflammatory Drugs (S. Garattini and M. N. G. Dukes, Eds.). Excerpta Medica, Amsterdam, 1964, pp. 207–213.Google Scholar
  11. 11.
    Claesson, S., Wettermark, G., and Juhlin, L. Action of ultraviolet light on skin: effect of the histamine liberator 48/80 and methotrimeprazine. Nature 183: 1451–1452, 1959.CrossRefGoogle Scholar
  12. 12.
    Veninga, T. S., and de Boer, J. E. Urinary excretion pattern of serotonin and 5-hydroxyindole acetic acid in ultraviolet induced erythema. J. Invest. Dermatol. 50: 1–8, 1968.Google Scholar
  13. 13.
    Logan, G., and Wilhelm, D. L. Vascular permeability changes in inflammation. I. The role of endogenous permeability factors in ultraviolet injury. Br. J. Exp. Pathol. 47: 300–314, 1966.Google Scholar
  14. 14.
    Epstein, J. H., and Winkelmann, R. K. Ultraviolet light-induced kinin formation in human skin. Arch. Dermatol. 95: 532–536, 1967.CrossRefGoogle Scholar
  15. 15.
    Søndergaard, J., and Greaves, M. W. Pharmacological studies in inflammation due to exposure to ultraviolet radiation. J. Pathol. 707: 93–97, 1970.CrossRefGoogle Scholar
  16. 16.
    Greaves, M. W., and Søndergaard, J. Pharmacologic agents released in ultraviolet inflammation studied by continuous skin perfusion. J. Invest. Dermatol. 54: 365–367, 1970.CrossRefGoogle Scholar
  17. 17.
    Jessup, S. J., McDonald-Gibson, W. J., Ramwell, P. W., and Shaw, J. E. Biosynthesis and release of prostaglandins on hormonal treatment of frog skin and their effect on ion transport. Fed. Proc. 29: 381, 1970.Google Scholar
  18. 18.
    Greaves, M. W., and McDonald-Gibson, W. J. Prostaglandin biosynthesis by human skin and its inhibition by corticosteroids. Br. J. Pharmacol. 46: 172–175, 1972.Google Scholar
  19. 19.
    Crunkhorn, P., and Willis, A. C. Cutaneous reactions to intradermal prostaglandins. Br. J. Pharmacol. 47: 49–56, 1971.Google Scholar
  20. 20.
    Mathur, G. P., and Gandhi, V. M. Prostaglandin in human and albino rat skin. J. Invest. Dermatol. 58: 291–295, 1972.CrossRefGoogle Scholar
  21. 21.
    Snyder, D. S., and Eaglstein, W. Topical indomethacin and sunburn. Br. J. Dermatol. 90: 91–93, 1974.CrossRefGoogle Scholar
  22. 22.
    Snyder, D. S. Cutaneous effects of topical indomethacin, an inhibitor of prostaglandin synthesis, on UV-damaged skin. J. Invest. Dermatol. 64: 322–325, 1975.CrossRefGoogle Scholar
  23. 23.
    Snyder, D. S., and Eaglstein, W. H. Intradermal anti-prostaglandin agents and sunburn. J. Invest. Dermatol. 62: 47–50, 1974.CrossRefGoogle Scholar
  24. 24.
    Eaglstein, W. H., and Marsico, A. R. Dichotomy in response to indomethacin in UV-C and UV-B induced ultraviolet light inflammation. J. Invest. Dermatol. 65: 238–240, 1975.CrossRefGoogle Scholar
  25. 25.
    Greenberg, R. A., Eaglstein, W. H., Turnier, H., and Houdek, P. V. Orally given indomethacin and blood flow response to UVL. Arch. Dermatol. 111: 328–330, 1975.CrossRefGoogle Scholar
  26. 26.
    Morison, W. L., Paul, B. S., and Parrish, J. A., The effects of indomethacin on longwave ultraviolet-induced delayed erythema. J. Invest. Dermatol. 68: 130–133, 1977.CrossRefGoogle Scholar
  27. 27.
    Miles, A. A. Lectures on the Scientific Basis of Medicine, Vol. 8. Athlone Press, London Uni-versity, 1958–59, p. 198.Google Scholar
  28. 28.
    Johnson, B. E., and Daniels, F., Jr. Lysosomes and the reactions of skin to ultraviolet radiation. J. Invest. Dermatol. 53: 85–94, 1969.Google Scholar
  29. 29.
    Sams, W. M., Jr. Inflammatory mediators in ultraviolet erythema. In Sunlight and Man: Normal and Abnormal Photobiologic Responses (M. A. Pathak, L. C. Harber, M. Seiji, and A. Kukita, Eds.; T. B. Fitzpatrick, Consulting Ed.). University of Tokyo Press, Tokyo, 1974, pp. 143–146.Google Scholar
  30. 30.
    Weissman, G., and Fell, H. B. The effect of hydrocortisone on the response of fetal rat skin in culture to ultraviolet irradiation. J. Exp. Med. 116: 365–380, 1962.CrossRefGoogle Scholar
  31. 31.
    Desai, I. D., Sawant, P. L., and Tappel, A. L. Peroxidative and radiation damage to isolated lysosomes. Biochim. Biophys. Acta 56: 277–285, 1964.CrossRefGoogle Scholar
  32. 32.
    Johnson, B. E. Ultraviolet radiation and lysosomes in skin. Nature 219: 1258–1259, 1968.CrossRefGoogle Scholar
  33. 33.
    Johnson, B. E., Mandel, G., and Daniels, F., Jr. Melanin and cellular reactions to ultraviolet radiation. Nature [New Biol.] 235: 147–149, 1972.Google Scholar
  34. 34.
    Wilgram, G. F., Kidd, R. L., Krawczyk, W. S., and Cole, P. L. Sunburn effect on keratinosomes. Arch. Dermatol. 707: 505–519, 1970.CrossRefGoogle Scholar
  35. 35.
    Nix, T. E., Jr., Nordquist, R. E., Scott, R. J., and Everett, M. A. Ultrastructural changes induced by ultraviolet light in human epidermis: basal and spinous layers. J. Invest. Dermatol. 45: 52–64, 1965.Google Scholar
  36. 36.
    Wolff, K., and Schreiner, E. Epidermal lysosomes: Electron microscopic-cytochemical studies. Arch. Dermatol. 707: 276–286, 1970.CrossRefGoogle Scholar
  37. 37.
    Fand, I. The protective effect of a sunscreen upon the lysosomes of ultraviolet irradiated skin. Dermatologica 144: 231–241, 1972.CrossRefGoogle Scholar
  38. 38.
    Allison, A. E., Magnus, I. A., and Young, M. R. Role of lysosomes and of cell membranes in photosensitization. Nature 209: 874–878, 1966.CrossRefGoogle Scholar
  39. 39.
    Lazarus, G. S., Hatcher, V. B., and Levine, N. Lysosomes and the skin. J. Invest. Dermatol. 65: 259–271, 1975.CrossRefGoogle Scholar
  40. 40.
    Allison, A. C. Lysosomes. In The Biological Basis of Medicine, Vol. 1 (E. E. Bittar and N. Bittar, Eds.). Academic Press, New York, 1969, pp. 209–242.Google Scholar
  41. 41.
    Hawkins, H. K., Ericsson, J. L. E., Biberfeld, P., and Trump, B. F. Lysosomes and phagosome stability in lethal cell injury. Am. J. Pathol. 68: 255–288, 1972.Google Scholar
  42. 42.
    Norins, A. Free radical formation in the skin following exposure to ultraviolet light. J. Invest. Dermatol. 39: 445–447, 1962.Google Scholar
  43. 43.
    Pathak, M. A., and Stratton, K. Free radicals in human skin before and after exposure to light. Arch. Biochem. Biophys. 723: 468–476, 1968.CrossRefGoogle Scholar
  44. 44.
    Miller, W. S., Ruderman, F. R., and Smith, J. G., Jr. Aspirin and ultraviolet light-induced erythema in man. Arch. Dermatol. 95: 357–358, 1967.CrossRefGoogle Scholar
  45. 45.
    Honigsmann, H., Wolff, K., and Konrad, K. Epidermal lysosomes and ultraviolet light. J. Invest. Dermatol. 63: 331–342, 1974.CrossRefGoogle Scholar
  46. 46.
    Ogura, R. M., and Knox, J. M. Biochemical changes in ultraviolet light-irradiated epidermis. In Sunlight and Man, Normal and Abnormal Photobiologic Responses (M. A. Pathak, L. C. Harber, M. Seiji, and A. Kukita, Eds.; T. B. Fitzpatrick, Consulting Ed.). University of Tokyo Press, Tokyo, 1974, pp. 147–156.Google Scholar
  47. 47.
    Cotran, R. S., and Majno, G. A light and electron microscopic analysis of vascular injury. Ann. N.Y. Acad. Sci. 776: 750–764, 1964.Google Scholar
  48. 48.
    Sams, W. M., Jr., and Winklemann, R. K. The effect of ultraviolet light on isolated cutaneous blood vessels. J. Invest. Dermatol. 53: 79–83, 1969.Google Scholar
  49. 49.
    Bottoms, E., and Shuster, S. Effect of ultraviolet light on skin collagen. Nature 799: 192–193, 1963.CrossRefGoogle Scholar
  50. 50.
    Consden, R., and Kirrane J. A. Action of ultraviolet light on soluble collagens. Nature 215: 165–166, 1967.CrossRefGoogle Scholar
  51. 51.
    van der Leun, J.C. Ultraviolet erythema: a study on diffusion processes in human skin. Thesis, Utrecht, 1966.Google Scholar
  52. 52.
    Lewis, T. H. The Blood Vessels of the Human Skin and Their Responses. Shaw, London, pp. 117–138, 1927.Google Scholar
  53. 53.
    Rottier, P. B. The erythematous action of ultraviolet light on human skin. I. Some measurements of the spectral response with continuous and intermittent light. J. Clin. Invest. 32: 681–689, 1953.CrossRefGoogle Scholar
  54. 54.
    Rottier, P. B. In Proceedings of the 1st International Photobiology Congress, Amsterdam, 1954, pp. 192–204.Google Scholar
  55. 55.
    Rottier, P. B. Untersuchungen mit einem kleinen Spektrographen zur Bestimmung der Hautempfindlichkeit gegenüber 300 und 250 mμ. Strahlentherapie 119: 591–606, 1962.Google Scholar
  56. 56.
    Miescher, G. Zur Histologie der lichtbedingten Reaktionen. Dermatologica 115: 345–357, 1957.CrossRefGoogle Scholar
  57. 57.
    Parrish, J. A., Pathak, M. A., Fine, S., and Neal, R. Ultraviolet laser irradiation of albino guinea pig skin. Program and Abstracts, First Annual Meeting of American Society for Photobiology, June 1973, p. 137.Google Scholar
  58. 58.
    Gahlen, W., and Bergmann, W. Uber den Protrahierungsfaktor beim U.V. Erythem. Arch. Derm. Syph. (Berlin) 200: 472–475, 1955.Google Scholar
  59. 59.
    Claesson, S., Juhlin, L., and Wettermark, G. The reciprocity law of UV-irradiation effects. Acta Derm. Venereol. (Stockh.) 55: 123–136, 1958.Google Scholar
  60. 60.
    Isenberg, J. N., and Fisch, R. O. Double-light phototherapy for neonatal hyperbilirubinemia. J. Pediatr. 55: 116–118, 1973.Google Scholar
  61. 61.
    Tan, K. L. Comparison of the effectiveness of single-direction and double-direction phototherapy for neonatal jaundice. Pediatrics 56: 550–553, 1975.Google Scholar
  62. 62.
    Olson, R. L., Sayre, R. M., and Everett, M. A. Effect of field size on ultraviolet minimal erythemal dose. J. Invest. Dermatol. 45: 516–517, 1965.CrossRefGoogle Scholar
  63. 63.
    Henschke, G. Die Winkelabhöngigkeit des Ultravioletterythems und ihre Bedeutung für die Dosimetrie. Strahlentherapie 77: 297–299, 1948.Google Scholar
  64. 64.
    Hausser, K. W., and Vahle, W. Sonnenbrand und Sonnenbräunung. Wissenschaftliche Veröffnungen des Siemens Konzern 6:101–120, 1927. English translation: Sunburn and suntan ning. In The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin) (F. Urbach, Ed.). Pergamon, Oxford, 1969, pp. 3–21.Google Scholar
  65. 65.
    Everett, M. A., Sayre, R. M., and Olson, R. L. Physiologic response of human skin to ultraviolet light. In The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin) (F. Urbach, Ed.). Pergamon, Oxford, 1969, pp. 181–186.Google Scholar
  66. 66.
    Sayre, R. M., Olson, R. L., and Everett, M. A. Quantitative studies on erythema. J. Invest. Dermatol. 46: 240–244, 1966.Google Scholar
  67. 67.
    Pathak, M. A., and Epstein, J. H. Normal and abnormal reactions of man to light. In Dermatology in General Medicine (T. B. Fitzpatrick, K. A. Arndt, W. H. Clark, Jr., A. Z. Eisen, E. J. Van Scott, and J. H. Vaughan, Eds.). McGraw-Hill, New York, 1971, pp. 977–1036.Google Scholar
  68. 68.
    Everett, M. A., Doran, C. K., Everett, H. D., and Anglin, J. H., Jr. Modification of sunburn by infrared rays. J.A.M.A. 756: 778–779, 1963.CrossRefGoogle Scholar
  69. 69.
    Owens, D. W., Knox, J. M., Hudson, H. T., and Troll, D. Influence of wind on ultraviolet injury. Arch. Dermatol. 109: 200–201, 1974.CrossRefGoogle Scholar
  70. 70.
    Owens, D. W., Knox, J. M., Hudson, H. T., and Troll, D. Influence of humidity on ultraviolet injury. J. Invest. Dermatol. 64: 250–252, 1975.CrossRefGoogle Scholar
  71. 71.
    Breit, R., and Kligman, A. M. Measurement of erythemal and pigmentary responses to ultraviolet radiution of different spectral qualities. In The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin) (F. Urbach, Ed.). Pergamon, Oxford, 1969, pp. 267–275.Google Scholar
  72. 72.
    Everett, M. A., Olson, R. L., and Sayre, R. M. Ultraviolet erythema. Arch. Dermatol. 92: 713–719, 1965.CrossRefGoogle Scholar
  73. 73.
    Bachem, A. Time factors of erythema and pigmentation, produced by ultraviolet rays of different wavelengths. J. Invest. Dermatol. 25: 215–218, 1955.CrossRefGoogle Scholar
  74. 74.
    Olson, R. L., Sayre, R. M., and Everett, M. A. Effect of anatomic location and time on ultraviolet erythema. Arch. Dermatol. 95: 211–215, 1966.CrossRefGoogle Scholar
  75. 75.
    Hausser, K. W., and Vahle, W. Die Abhängigkeit des Lichterythems und der Pigmentbildung von der Schwingungszahl (Wellenlänge) der erregenden Strahlung. Strahlentherapie 73: 41–71, 1922.Google Scholar
  76. 76.
    Rottier, P. B. The ratio of the 260/300 nm MED’s in the judgement of skin sensitivity to short wave ultraviolet radiation. In The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin) (F. Urbach, Ed.). Pergamon, Oxford, 1969, pp. 187–195.Google Scholar
  77. 77.
    Adams, E. Q., Barnes, B. T., and Forsythe, W. E. Uber die Erythemwirksamkeit ultravioletten Lichtes. Strahlentherapie 48: 235–249, 1933.Google Scholar
  78. 78.
    Parrish, J. A. Expanding the clinical spectrum of sunscreen testing. Cutis 5: 498–506, 1971.Google Scholar
  79. 79.
    Luckiesh, M. Application of Germicidal Erythemal and Infrared Energy. Van Nostrand, New York, 1946.Google Scholar
  80. 80.
    Buttolph, L. J. Practical applications and sources of ultraviolet energy. In Radiation Biology, Vol. 2 (A. Hollaender, Ed.). McGraw-Hill, New York, 1955, pp. 41–46.Google Scholar
  81. 81.
    Hausser, I. Uber spezifische Wirkungen des langwelligen ultravioletten Lichts auf die Menschliche Haut. Strahlentherapie 62: 315–322, 1938.Google Scholar
  82. 82.
    Meyer, A. E. H., and Seitz, E. O. Ultraviolette Strahlen, de Gruyter, Berlin, 1949, pp. 227–251.Google Scholar
  83. 83.
    van der Leun, J. C. Theory of ultraviolet erythema. Photochem. Photobiol. 4: 453–458, 1965.CrossRefGoogle Scholar
  84. 84.
    Parrish, J. A., Ying, C. Y., Pathak, M. A., and Fitzpatrick, T. B. Erythemogenic properties of long-wave ultraviolet light. In Sunlight and Man: Normal and Abnormal Photobiologic Responses (M. A. Pathak, L. C. Harber, M. Seiji, and A. Kukita, Eds.; T. B. Fitzpatrick, Consulting Ed.). University of Tokyo Press, Tokyo, 1974, pp. 131–141.Google Scholar
  85. 85.
    Ying, C. Y., Parrish, J. A., and Pathak, M. A. Additive erythemogenic effects of middle- (280–320) and (320–400 nm) long-wave ultraviolet light. J. Invest. Dermatol. 63: 273–278, 1974.CrossRefGoogle Scholar
  86. 86.
    Parrish, J. A., Anderson, R. R., Ying, C. Y., and Pathak, M. A. Cutaneous effects of pulsed nitrogen gas laser irradiation. J. Invest. Dermatol. 67: 603–608, 1976.CrossRefGoogle Scholar
  87. 87.
    Juhlin, L. Abstract, German-Swedish Symposium on Photomedicine, Frankfurt, Germany, April 23–25, 1975.Google Scholar
  88. 88.
    Tanenbaum, L., Parrish, J. A., Pathak, M. A., Anderson, R. R., and Fitzpatrick, T. B. Tar photoxicity and phototherapy for psoriasis. Arch. Dermatol. 111: 461–470, 1975.Google Scholar
  89. 89.
    Willis, I., and Cylus, L. UV-A erythema in skin: Is it a sunburn? J. Invest. Dermatol. 68: 128–129, 1977.CrossRefGoogle Scholar
  90. 90.
    Rosario, R., and Shea, S. Unpublished observations.Google Scholar
  91. 91.
    Bücher, H. Zur Abgrenzung des UV-Erythems durch das unspezifische Strahlungserythem. Strahlentherapie 111: 404–413, 1960.Google Scholar
  92. 92.
    van der Leun, J. C., and Stoop, T. Photorecovery of ultraviolet erythema. In The Biologie Effects of Ultraviolet Radiation (with Emphasis on the Skin) (F. Urbach, Ed.). Pergamon Press, Oxford, 1969, pp. 251–254.Google Scholar
  93. 93.
    Willis, I., Kligman, A., and Epstein, J. Effects of long ultraviolet rays on human skin: Photoprotective or photoaugmentative? J. Invest. Dermatol. 59: 416–420, 1972.CrossRefGoogle Scholar
  94. 94.
    Adams, E. Q., Barnes, B. T., and Forsythe, W. E. Erythema due to ultraviolet radiation. J. Opt. Soc. Am. 27: 207–222, 1931.CrossRefGoogle Scholar
  95. 95.
    Criteria for Recommended Standard-Occupational Exposure to Ultraviolet Radiation. US-DHEW, PHS, NIOSH HSM-73-11009, 1972.Google Scholar
  96. 96.
    Stern, W. K. Anatomic localization of the response to ultraviolet radiation in human skin. Dermatologica 745: 361–370, 1972.CrossRefGoogle Scholar
  97. 97.
    Stern, W. K., and Urbach, F. The diagnostic significance of minimal erythemal dose. Arch. Dermatol. 705: 387–393, 1972.CrossRefGoogle Scholar
  98. 98.
    Rosario, R., Mark, G. J., Parrish, J. A., and Mihm, M. C., Jr. Histologic changes produced in skin by equally erythemogenic doses of UV-A, UV-B, UV-C, and UV-A with psoralens. Submitted for publication.Google Scholar
  99. 99.
    Kumakiri, M., Hashimoto, K., and Willis, I. Biologic changes due to long-wave ultraviolet irradiation on human skin: Ultrastructural study. J. Invest. Dermatol. 69: 392–400, 1977.CrossRefGoogle Scholar
  100. 100.
    Baden, H. P., and Pearlman, C. The effect of ultraviolet light on protein and nucleic acid synthesis in the epidermis. J. Invest. Dermatol. 45: 71–75, 1964.Google Scholar
  101. 101.
    Fukuyama, K., Epstein, W. L., and Epstein, J. H. Effect of ultraviolet light on RNA and protein synthesis in differentiated epidermal cells. Nature 216: 1031–1032, 1967.CrossRefGoogle Scholar
  102. 102.
    Epstein, J. H., Fukuyama, K., and Epstein, W. L. UVL induced stimulation of DNA synthesis in hairless mouse epidermis. J. Invest. Dermatol. 51: 445–453, 1968.Google Scholar
  103. 103.
    Epstein, W. L., Fukuyama, K., and Epstein, J. H. Early effects of ultraviolet light on DNA synthesis in human skin in vivo. Arch. Dermatol. 100: 84–89, 1969.CrossRefGoogle Scholar
  104. 104.
    Epstein, J. H., Fukuyama, K., and Fye, K. Effects of ultraviolet radiation on the mitotic cycle and DNA, RNA and protein synthesis in mammalian epidermis in vivo. Photochem. Photobiol. 12: 51–65, 1970.CrossRefGoogle Scholar
  105. 105.
    Trosko, J. E., and Isoun, M. Photosensitizing effects of trisoralen on DNA synthesis in human cells grown in vitro. Int. J. Radiat. Biol. 79: 87–92, 1971.CrossRefGoogle Scholar
  106. 106.
    Baden, H. P., Parrington, J. M., Delhanty, J. D. A., and Pathak, M. A. DNA synthesis in normal and xeroderma pigmentosum fibroblasts following treatment with 8-methoxypsoralen and longwave ultraviolet light. Biochem. Biophys. Acta 262: 247–255, 1972.Google Scholar
  107. 107.
    Walter, J. F., Voorhees, J. J., Kelsey, W. H., and Duell, E. A. Psoralen plus black light inhibits epidermal DNA synthesis. Arch. Dermatol. 707: 861–865, 1973.CrossRefGoogle Scholar
  108. 108.
    Epstein, J. H., and Fukuyama, K. Effects of 8-methoxypsoralen-induced phototoxic effects on mammalian epidermal macromolecular synthesis in vivo. Photochem. Photobiol. 21: 325–330, 1975.CrossRefGoogle Scholar
  109. 109.
    Payne, F. T. An evaluation of actinic blocking agents for the protection of lip mucosa. J. Am. Dent. Assoc. 92: 409–411, 1976.Google Scholar
  110. 110.
    Parrish, J. A. Unpublished observations, 1974.Google Scholar
  111. 111.
    Lampert, F., and Loew, R. Physicalische und biologische Prüfung des Nuva-Lite-Gerätes. Z.W.R. 83: 696–699, 1974.Google Scholar
  112. 112.
    Quevedo, W. C., Jr., Fitzpatrick, T. B., Pathak, M. A., and Jimbow, K. Light and skin color. In Sunlight and Man: Normal and Abnormal Photobiologic Responses (M. A. Pathak, L. C. Harber, M. Seiji, and A. Kukita, Eds.; T. B. Fitzpatrick, Consulting Ed.). University of Tokyo Press, Tokyo, 1974, pp. 165–194.Google Scholar
  113. 113.
    Pathak, M. A., Riley, F. J., Fitzpatrick, T. B., and Curwen, W. L. Melanin formation in human skin induced by long-wave ultraviolet and visible light. Nature 793: 148–150, 1962.CrossRefGoogle Scholar
  114. 114.
    Pathak, M. A. Photobiology of melanogenesis: Biophysical aspects. In Advances in Biology of Skin, Vol. 8 (W. Montagna and F. Hu, Eds.). Pergamon Press, Oxford, 1967, pp. 397–420.Google Scholar
  115. 115.
    Pathak, M. A., Hori, Y., Szabo, G., and Fitzpatrick, T. B. The photobiology of melanin pig-mentation in human skin. In Biology of Normal and Abnormal Melanocytes (T. Kawamura, T. B. Fitzpatrick, and M. Seiji, Eds.). University of Tokyo Press, Tokyo, 1971, pp. 149–167.Google Scholar
  116. 116.
    Jimbow, K., Pathak, M. A., and Fitzpatrick, T. B. Effect of ultraviqlet on the distribution pattern of microfilaments and microtubules and on the nucleus in human melanocytes. Yale J. Biol. Med. 46: 411–426, 1973.Google Scholar
  117. 117.
    Parrish, J. A., Pathak, M. A., and Fitzpatrick, T. B. Topical protection against germicidal radiation. Arch. Surg. 704: 276–283, 1972.Google Scholar
  118. 118.
    Konrad, K., and Wolff, K. Hyperpigmentation, melanosome size, and distribution patterns of melanosomes. Arch. Dermatol. 107: 853–860, 1973.CrossRefGoogle Scholar
  119. 119.
    Flaxman, B. A., Sosis, A. C., and Van Scott, E. J. Changes in melanosome distribution in Caucasoid skin following topical application of nitrogen mustard. J. Invest. Dermatol. 60: 321–326, 1973.CrossRefGoogle Scholar
  120. 120.
    Toda, K., Pathak, M. A., Parrish, J. A., Fitzpatrick, T. B., and Quevedo, W. C., Jr. Alteration of racial differences in melanosome distribution in human epidermis after exposure to ultraviolet light. Nature [New Biol.] 236: 143–145, 1972.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • John A. Parrish
    • 1
  • R. Rox Anderson
    • 1
  • Frederick Urbach
    • 2
  • Donald Pitts
    • 3
  1. 1.Harvard Medical SchoolUSA
  2. 2.Skin and Cancer HospitalTemple University School of MedicineUSA
  3. 3.College of OptometryUniversity of HoustonUSA

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