Coupling Factors in Oxidative Phosphorylation

  • D. Rao Sanadi
  • Saroj Joshi
  • Fariyal M. Shaikh


A classical approach to the study of the mechanism of multienzyme systems involves resolution of the constituent parts and reconstitution of the system from the purified components. Application of this approach to the study of the mechanism of oxidative phosphorylation was first initiated in David Green’s laboratory. He devised the procedures for the large-scale isolation of mitochondria from bovine heart.(1) Even now this is the source for much of the fractionation studies. In 1958 the preparation of phosphorylating submitochondrial particles, ETPH, and the first coupling factor(2) was reported. General use of these pioneering techniques led to the isolation and characterization of four generally accepted coupling factors, shown in Table I.


ATPase Activity Oxidative Phosphorylation Coupling Factor None None Inhibitor Content 
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  1. 1.
    Crane, F. L., Glenn, J. L., and Green, D. E. (1956) Biochim. Biophys. Acta 22, 475.PubMedCrossRefGoogle Scholar
  2. 2.
    Linnane, A. W. (1958) Biochim. Biophys. Acta 30, 221.PubMedCrossRefGoogle Scholar
  3. 3.
    Sanadi, D. R., and Fluharty, A. L. (1963) Biochemistry 2, 523.PubMedCrossRefGoogle Scholar
  4. 4.
    Andreoli, T. E., Lam, K. W., and Sanadi, D. R. (1965)J. Biol. Chem. 240, 2644.PubMedGoogle Scholar
  5. 5.
    Pullman, M. E., and Monroy, G. C. (1963)J. Biol. Chem. 238. 3762.PubMedGoogle Scholar
  6. 6.
    Warshaw, J. B., Lam, K. W., Nagy, B., and Sanadi, D. R. (1968) Arch. Biochem. Biophys. 123, 385.PubMedCrossRefGoogle Scholar
  7. 7.
    Pullman, M. E., and Schatz, G. (1976) Ann. Rev. Biochem. 36, 539.PubMedCrossRefGoogle Scholar
  8. 8.
    Penefsky, M. S., (1967) J. Biol. Chem. 242, 5789.PubMedGoogle Scholar
  9. 9.
    Beechey, R. B. (1974) in Membrane ATPases and Transport Processes (Bronk, J. R., ed.), p. 41, Biochemical Society Special Publication No. 4, The Biochemical Society, London.Google Scholar
  10. 10.
    Horstman, L. L., and Racker, E. (1970)J. Biol. Chem. 245, 1336.PubMedGoogle Scholar
  11. 11.
    Chan, S. H. P., and Barbour, R. L. (1976) Biochem. Biophys. Res. Commun. 72, 499.PubMedCrossRefGoogle Scholar
  12. 12.
    Adolfsen, R. A., McClung, J. A., and Moudrianakis, E. N. (1975) Biochemistry 14, 1727.Google Scholar
  13. 13.
    Lam, K. W., Warshaw, J. B., and Sanadi, D. R. (1967) Arch. Biochem. Biophys. 119, 477.CrossRefGoogle Scholar
  14. 14.
    Lam, K. W., Swann, D., and Elzinga, M. (1969) Arch. Biochem. Biophys. 130, 175.PubMedCrossRefGoogle Scholar
  15. 15.
    Lam, K. W., and Yang, S. S. (1969) Arch. Biochem. Biophys. 133, 366.PubMedCrossRefGoogle Scholar
  16. 16.
    Shankaran, R., Sani, B. P., and Sanadi, D. R. (1975) Arch. Biochem. Biophys. 168, 394.PubMedCrossRefGoogle Scholar
  17. 17.
    You, K., and Hatefi, Y. (1976) Biochim. Biophys. Acta 423, 398.PubMedCrossRefGoogle Scholar
  18. 18.
    Tzagoloff, A., Byington, K. H., and MacLennan, D. H. (1968)J. Biol. Chem. 243, 2405.Google Scholar
  19. 19.
    Sadler, M. H., Hunter, D. R., and Haworth, R. A. (1974) Biochem. Biophys. Res. Commun. 59, 804.PubMedCrossRefGoogle Scholar
  20. 20.
    Hatefi, Y., Stiggall, D. L., Galante, Y., and Hanstein, W. G. (1974) Biochem. Biophys. Res. Commun. 61, 313.Google Scholar
  21. 21.
    Serrano, R., Kanner, B. I., and Racker, E. (1976) J. Biol. Chem. 251, 2453.PubMedGoogle Scholar
  22. 22.
    Racker, E., Chien, T. F., and Kandrach, A. (1975) FEBS Lett. 57, 14.PubMedCrossRefGoogle Scholar
  23. 23.
    Joshi, S., Shaikh, F. M., and Sanadi, D. R. (1975) Biochem. Biophys. Res. Commun. 65, 1371.PubMedCrossRefGoogle Scholar
  24. 24.
    Tzagoloff, A., MacLennan, D. H., and Byington, K. H. (1968) Biochemistry 7, 1596.PubMedCrossRefGoogle Scholar
  25. 25.
    Lee, C. P., and Ernster, L. (1965) Biochem. Biophys. Res. Commun. 18, 523.PubMedCrossRefGoogle Scholar
  26. 26.
    Melnick, R. L., DeSousa, J. T., Maguire, J., and Packer, L. (1975) Arch. Biochem. Biophys. 166, 139.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • D. Rao Sanadi
    • 1
    • 2
  • Saroj Joshi
    • 1
    • 2
  • Fariyal M. Shaikh
    • 1
    • 2
  1. 1.Department of Cell PhysiologyBoston Biomedical Research InstituteBostonUSA
  2. 2.Department of Biological ChemistryHarvard Medical SchoolBostonUSA

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