Cytopathology of Human Gastrointestinal Cancers

  • Steven I. Hajdu
Part of the Sloan-Kettering Institute Cancer Series book series (SKICS)


Since the development of Papanicolaou’s technique for cytological examination of vaginal smears for the diagnosis of uterine carcinoma, exfoliative cytological techniques have been successfully employed for the detection of uterine, pulmonary, and genitourinary tumors. Exfoliative cytological techniques can be applied to gastrointestinal tumors with more or less similar results to those for other anatomical sites (Ackerman, 1967; Brandborg and Wenger, 1968; Lemon, 1952; Raskin et al, 1959; Rubin et al, 1953; Sherlock et al, 1972; Winawer et al, 1976; Witte, 1970; Yamakawa et al, 1971; Yoshii et al, 1970, 1971).


Ethyl Alcohol Pernicious Anemia Biliary Tract Cancer Cytologic Diagnosis Absolute Ethyl Alcohol 
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  1. Ackerman, N. B., 1967, An evaluation of gastric cytology: Results of a nationwide survey, J . Chron. Dis. 20: 621–626.PubMedCrossRefGoogle Scholar
  2. Anthonisen, P., and Riis, P., 1962, Cytology of colonic secretion in proctosigmoidal disease, Acta Med. Scand. 172: 375–381.PubMedCrossRefGoogle Scholar
  3. Attah, E. B., and Hajdu, S. I., 1968, Benign and malignant tumors of the esophagus at autopsy, J. Thorac. Cardiovasc. Surg. 55: 396–404.PubMedGoogle Scholar
  4. Ayre, J. E., and Oren, B. G., 1953, A new rapid method for stomach cancer diagnosis: The gastric brush, Cancer 6: 1177–1181.PubMedCrossRefGoogle Scholar
  5. Ayre, J. E., and Oren, B. G., 1957, Colon brush: A new diagnostic procedure for cancer of the lower bowel, Am. J. Digest. Dis. 2: 74–80.PubMedCrossRefGoogle Scholar
  6. Bader, G. M., and Papanicolaou, G. N., 1952, Application of cytology in diagnosis of cancer of rectum, sigmoid and descending colon, Cancer 5: 307–314.PubMedCrossRefGoogle Scholar
  7. Belladonna, J. A., Hajdu, S. I., Bains, M. S., and Winawer, S. J., 1974, Adenocarcinoma in situ of Barrett’s esophagus diagnosed by endoscopic cytology, N. Engl. J. Med. 291: 895–896.PubMedCrossRefGoogle Scholar
  8. Benvenuti, G. A., Hattori, K., Levin, B., Kirsner, J. B., and Reilly, R. W., 1975, Endoscopic sampling for tissue diagnosis in gastrointestinal malignancy, Gastrointest. Endoscofry 21: 159–161.CrossRefGoogle Scholar
  9. Bennington, J. L., Porus, R., Ferguson, B., and Hannon, G., 1968, Cytology of gastric sarcoid, Acta Cytol. 12: 30–36.PubMedGoogle Scholar
  10. Blank, W. A., and Steinberg, A. H., 1951, Cytologic diagnosis of malignancies of the lower bowel and rectum, Am. J. Surg. 81: 127–131.PubMedCrossRefGoogle Scholar
  11. Boddington, M. M., and Truelove, S. C., 1956, Abnormal epithelial cells in ulcerative colitis, Br. Med. J. 1: 1318–1321.PubMedCrossRefGoogle Scholar
  12. Boen, S. T., 1957, Changes in nuclei of squamous epithelial cells in pernicious anemia Acta Med. Scand. 159:425–431. PubMedCrossRefGoogle Scholar
  13. Bowden, L., and Papanicolaou, G. N., 1959, Exfoliated pancreatic cancer cells in the duct of Wirsung, Ann. Surg. 150: 296–98.PubMedCrossRefGoogle Scholar
  14. Bowden, L., and Papanicolaou, G. N., 1960, The diagnosis of pancreatic cancer by cytology study of duodenal secretions, Acta Un. Int. Caner. 16: 398–404.Google Scholar
  15. Brandborg, L. L., and Wenger, J., 1968, Cytological examination in gastrointestinal tract disease Med. Clin. N. Am. 52:1315–1328. PubMedGoogle Scholar
  16. Burn, J. I., 1961, Exfolative cytology of the colon Proc. R. Soc. Med. 54:726–729. PubMedGoogle Scholar
  17. Cabre-Fiol, V., and Olo-Garcia, R., 1962, Citodiagnostico de las neoplasias gastricas malignas por biopsia exfoliativa, Rev. Esp. Engerm. Apar. Dig. 21: 571.Google Scholar
  18. Cameron, A. B., 1960, A cytologic method of diagnosis of carcinoma of the colon Dis. Colon Rectum3:230–236.PubMedCrossRefGoogle Scholar
  19. Cameron, A. B., and Thabet, R. J., 1959, Recovery of malignant cells from enema returns in carcinoma of colon, Surg. Forum 10: 30–33.Google Scholar
  20. Cameron, J., and Hajdu, S. I., 1977, Cytology of esophageal and gastric carcinomas, in press. Deschner, E. E., Long, F. C., and Katz, S., 1973, Autoradiographic method for an expanded assessment of colonic cytology, Acta Cytol 17:435–438.Google Scholar
  21. Dreiling, D. A., Nieburgs, H. E., and Janowitz, H. D., 1960, The combined secretin and cytology test in the diagnosis of pancreatic and biliary tract cancer, Med. Clin. N. Am. 44: 801–815.PubMedGoogle Scholar
  22. Eras, P., Goldstein, M. J., and Sherlock, P., 1972, Candida infection of the gastrointestinal tract, Medicine 51: 367–379.PubMedCrossRefGoogle Scholar
  23. Foushee, J. H. S., Kalnins, Z. A., Dixon, F. R., Girsh, S., Morehead, R. P., O’Brien, T. F., Pribor, H., and Tattory, C., 1969, Gastric cytology: Evaluation of methods and results in 1,670 cases, Acta Cytol. 13: 399–406.PubMedGoogle Scholar
  24. Fukuda, T., Shida, S., Takita, T., and Sawada, Y., 1967, Cytologic diagnosis of early gastric cancer by the endoscope method with gastrofiberscope, Acta Cytol. 11: 456–459.PubMedGoogle Scholar
  25. Galambos, J. T., 1962, Cytologic examination of benign colonic lesions, Acta Cytol. 6: 148–54.PubMedGoogle Scholar
  26. Galambos, J. T., and Klayman, M. I., 1955, The clinical value of colonic exfoliative cytology in the diagnosis of cancer beyond the reach of the proctoscope. Surg. Gynecol. Obstet. 101: 673–679.PubMedGoogle Scholar
  27. Gardner, F. N., 1956, Observations on the cytology of gastric epithelium in tropical sprueJ. Lab. Clin. Med. 47:529–539. PubMedGoogle Scholar
  28. Gephart, T., and Graham, R. M., 1959, The cellular detection of carcinoma of the esophagus Surg. Gynecol. Obstet. 108:75–82. PubMedGoogle Scholar
  29. Goldgraber, M. B., Rubin, C. E., and Owens, F. J., 1953, The cytological diagnosis of duodenal sarcoma (polymorphic reticulosarcoma), Ann. Intern. Med. 39: 1316–1322.PubMedCrossRefGoogle Scholar
  30. Goldstein, H., and Ventzke, L. E., 1968, Value of exfoliative cytology in pancreatic carcinoma Gut 9:316–318. PubMedCrossRefGoogle Scholar
  31. Graham, R. M., and Rheault, M. H., 1954, Characteristic cellular changes in epithelial cells in pernicious anemia, J. Lab. Clin. Med. 43: 235–245.PubMedGoogle Scholar
  32. Hajdu, S. I., and Hajdu, E. 0., 1976 Cytopathology of Sarcomas and Other Nonepithelial Malignant TumorsSaunders, Philadelphia. Google Scholar
  33. Hajdu, S. I., Erlandson, R. A., and Paglia, M. A., 1972, Light and electron microscopic studies of gastric leiomyoblastoma, Arch. Pathol. 93: 36–41.PubMedGoogle Scholar
  34. Hajdu, S. I., Bean, M. A., Fogh, J., Hajdu, E. O., and Ricci, A., 1974a, Papanicolaou smear of cultured human tumor cells, Acta Cytol. 18: 327–332.PubMedGoogle Scholar
  35. Hajdu, S. I., Winawer, S. J., and Myers, W. P. L., 1974b, Carcinoid tumors: A study of 204 cases Am. J. Clin. Pathol. 61:521–528. PubMedGoogle Scholar
  36. Heindenreich, A., 1961, Rectocolic exfoliative cytology, Prensa Med. Argent. 47: 2009–2019.Google Scholar
  37. Hemmeter, J., 1889, The early diagnosis of cancer of the stomach, Med. Rec. (N.Y.) 46: 577.Google Scholar
  38. Henning, N., Witte, S., and Bressel, D., 1964, The cytological diagnosis of tumors of the upper gastrointestinal tract (esophagus, stomach, duodenum), Acta Cytol. 8:121–130.PubMedGoogle Scholar
  39. Hirschowitz, B. I., Curtis, L. I., Peters, C. W., and Pollard, H. M., 1958, Demonstration of a new gastroscope, the “fibroscope,” Gastroenterology 35:50–53.Google Scholar
  40. Johnson, W. D., Koss, L. G., Papanicolaou, G. N., and Seybolt, J. F., 1955, Cytology of esophageal washings: Evaluation of 364 cases, Cancer 8: 951–957.PubMedCrossRefGoogle Scholar
  41. Kasugai, T., 1968, Evaluation of gastric lavage cytology under direct-vision by the fibergastroscope employing Hands’ solution as a washing solution, Acta Cytol. 12: 345–351.PubMedGoogle Scholar
  42. Katz, S., Boyle, C. C., Sherlock, P., and Winawer, S., 1972, Gastric exfoliative cytology, a rapid method, Gastroenterology 62: 85.Google Scholar
  43. Klayman, M. I., Kirsner, J. B., and Palmer, W. L., 1955, Gastric malignant lymphoma: Increasing accuracy in diagnosis, Gastroenterology 29: 536–547.PubMedGoogle Scholar
  44. Klein, M. S., and Sherlock, P., 1972, Gastric and colonic metastases from breast cancer, Am. J. Digest. Dis. 17: 881–886.PubMedCrossRefGoogle Scholar
  45. Knoerschild, H. E., and Cameron, A. B., 1963, Mucosal smear cytology in the detection of colonic carcinoma, Acta Cytol. 7: 233–235.Google Scholar
  46. Knoerschild, H. E., Cameron, A. B., and Zollinger, R. M., 1961, Millipore filtration of colonic washings in malignant lesions of the large bowel, Am. J. Surg. 101: 20–22.CrossRefGoogle Scholar
  47. Kobayashi, S., Prolla, J. C., and Kirsner, J. B., 1970, Brushing cytology of the esophagus and stomach under direct-vision by fiberscopes, Acta Cytol. 14: 219–223.PubMedGoogle Scholar
  48. Lemon, H. M., 1952, The application of cytologic diagnosis to cancers of the stomach, pancreas and biliary system, Ann. Intern. Med. 37: 525–533.PubMedCrossRefGoogle Scholar
  49. Lemon, H. M., and Byrnes, W. W., 1949, Cancer of the biliary tract and pancreas: Diagnosis from cytology of duodenal aspiration, Am. Med. Assoc. J.141: 254–257.CrossRefGoogle Scholar
  50. Loeper, M., and Binet, E., 1911, Le cytodiagnostic des affections de l’estomac, Bull. Soc. Med. Hop. (Paris) 31: 563–574.Google Scholar
  51. MacKenzie, L. L., and Miller, H. B., 1949, Primary carcinoma of the duodenum: Report of a case in which malignant cells were recovered by duodenal drainage, Gastroenterology 12: 309–311.PubMedGoogle Scholar
  52. Maimon, H. N., Dreskin, R. B., and Cocco, A. E., 1974, Positive esophageal cytology without detectable neoplasm, Gastrointest. Endoscopy 20: 156–59.CrossRefGoogle Scholar
  53. Marini, G., 1909, Ueber die Diagnose des Magencarcinomas auf Grund der Cytologischen des Spulwasser, Arch. Verdaumgskrankh. 15: 251–268.CrossRefGoogle Scholar
  54. McNeer, G., and Ewing, J. H., 1949, Exfoliated pancreatic cancer cells in duodenal drainage: Case report, Cancer 2: 643–645.PubMedCrossRefGoogle Scholar
  55. Messelt,O.T., 1960, Results of the cytologic diagnosis of esophageal cancer by smears from material obtained by esophagoscopy: Evaluation of 414 cases, Acta Un. Int. Cancr. 16:1364–1367.Google Scholar
  56. Nelson, R. S., and Lanza, F. L., 1974, The endoscopie diagnosis of gastric lymphoma, Gastrointest. Endoscopy 21: 66–68.CrossRefGoogle Scholar
  57. Nieburgs, H. E., Dreiling, D. A., Rubio, C., and Reisman, R., 1962, The morphology of cells in duodenal drainage smears: Histologic origin and pathologic significance, Am J . Digest. Dis. 7: 489–505.PubMedCrossRefGoogle Scholar
  58. Oakland, D. J., 1961, The diagnosis of carcinoma of the large bowel of exfoliative cytology, Br J . Surg. 48: 353–362.PubMedCrossRefGoogle Scholar
  59. Orell, S. R., and Ohlsen, P., 1972, Normal and post-pancreatic cytologic patterns of the duodenal juice, Acta Cytol. 16: 165–171.PubMedGoogle Scholar
  60. Papanicolaou, G. N., and Cooper, W. A., 1947, The cytology of the gastric fluid in the diagnosis of carcinoma of the stomach, J. Natl. Cancer Inst. 7: 357–360.PubMedGoogle Scholar
  61. Prolla, J. C., Taebel, D. W., and Kirsner, J. B., 1965, Current status of exfoliative cytology in diagnoses of malignant neoplasms of the esophagus, Surg. Gynecol. Obstet. 121: 743–752.PubMedGoogle Scholar
  62. Prolla, J. C., Kobayashi, S., and Kirsner, J. B., 1969, Gastric cancer: Some recent improvements in diagnosis based upon the Japanese experience, Arch. Intern. Med. 124: 238–246.PubMedCrossRefGoogle Scholar
  63. Prolla, J. C., Kobayashi, S., and Kirsner, J. B., 1970, Cytology of malignant lymphomas of the stomach, Acta Cytol. 14: 291–296.PubMedGoogle Scholar
  64. Raskin, H F., Kirsner, J. B., and Palmer, W. L., 1959, Role of exfoliative cytology in the diagnosis of cancer of the digestive tract, Am. Med. Assoc. 169:789–791.CrossRefGoogle Scholar
  65. Reed, P. I Raskin, H. F., and Graff, P., 1962, Malignant melanoma of the stomach, Am. Med. Assoc. 182:178–179 CrossRefGoogle Scholar
  66. Rosen, R. G., Garret, M., and Aka, E., 1968, Cytologic diagnosis of pancreatic cancer by ductal aspiration, Ann. Surg. 167: 427–432.PubMedCrossRefGoogle Scholar
  67. Rubin, C. E., 1955, The diagnosis of gastric malignancy in pernicious anemia, Gastroenterology 29: 563–587.PubMedGoogle Scholar
  68. Rubin, C. E., and Massey, B. W., 1954, Preoperative diagnosis of gastric and duodenal malignant lymphoma by exfoliative cytology, Cancer 7: 271–288.PubMedCrossRefGoogle Scholar
  69. Rubin, C. E., Massey, B. W., and Kirsner, J. B., 1953, The clinical value of gastrointestinal cytology diagnosis, Gastroenterology 25: 119–138.PubMedGoogle Scholar
  70. Seybolt, J. F., Papanicolaou, G. N., and Cooper, W. A., 1951, Cytology in diagnosis of gastric cancer, Cancer 4: 286–295.PubMedCrossRefGoogle Scholar
  71. Sherlock, P., Ehrlich, A. N., and Winawer, S. J., 1972, Diagnosis of gastrointestinal cancer: Current status and recent progress, Gastroenterology 63: 672–700.PubMedGoogle Scholar
  72. Simon, P., and Caussade, L., 1914, Le cytodiagnostic du cancer de l’estomac, Presse Med. 22: 265–285.Google Scholar
  73. Spjut, H. J., Margolis, A. A., and Cook, G. B., 1963, The silicone foam enema: A source for exfoliative cytologic specimens, Acta Cytol. 7: 79–84.PubMedGoogle Scholar
  74. Takenaka, M., and Ayabe, M., 1970, Effect of carcinostatic agents on exfoliated cancer cells of the stomach, Acta Cytol. 14: 589–594.PubMedGoogle Scholar
  75. Wiendl, H. J., Shwabe, M., Becker, G., and Kowatsch, J., 1974, Feulgen-cytophotometric studies of gastric mucosal smears in malignant and benign diseases of the stomach, Acta Cytol. 18: 222–230.PubMedGoogle Scholar
  76. Winawer, S. J., Fleisher, M., Melamed, M. R., Sherlock, P., Deschner, E., and Schwartz, M., 1974a, Cytological, immunological (CEA) and isotope labeling studies based on gastrointestinal endoscopic lavage, Gastroenterology 66: 2–146 /800.Google Scholar
  77. Winawer, S. J., Mak, M. S., and Boyle, C., 1974b, Directed brush cytology in the diagnosis of recurrent gastric adenocarcinoma using a forward-viewing panendoscope, Gastroenterology 66: 2–176 /830.Google Scholar
  78. Winawer, S. J., Sherlock, P., Belladonna, J. A., Melamed, M. R., and Beattie, E. J., Jr., 1975, Endoscopic brush cytology in esophageal cancer, Am. Med. Assoc. 232: 1358.CrossRefGoogle Scholar
  79. Winawer, S.J., Sherlock, P., and Hajdu, S. I., 1976, Endoscopy in the diagnosis and management of patients with upper gastrointestinal cancer, Cancer 37: 440–448.PubMedCrossRefGoogle Scholar
  80. Witte, S., 1970, Gastroscopic cytology, Endoscopy 2: 88–93.CrossRefGoogle Scholar
  81. Yamakawa, T., Panish, J., Berci, G., Morgenstern, L., et al,1971, The correlation of target biopsy and contact smear cytology under direct visual control in malignant gastric lesions, Gastrointest. Endoscopy 17:164–168. Google Scholar
  82. Yoshii, Y., Takahashi, J., Yamaoka, Y., and Kasugai, T., 1970, Significance of imprint smears in cytologic diagnosis of malignant tumors of the stomach, Acta Cytol. 14: 249–253.PubMedGoogle Scholar
  83. Yoshii, Y., Kobuyoshi, K., Yagi, M., and Kasugai, T., 1971, Endoscopy, biopsy and cytology in esophageal and gastric carcinoma with the fiberesophagoscope, Gastrointest. Endoscopy, 17: 150–152Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Steven I. Hajdu
    • 1
    • 2
  1. 1.Memorial Sloan-Kettering Cancer CenterNew YorkUSA
  2. 2.Cornell University Medical CollegeNew YorkUSA

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