Morphology of Membrane Systems in Pancreatic Islets

  • Lelio Orci
  • Alain Perrelet


In the last few years, membrane systems have been identified as key effectors in cell function. In parallel with this awareness of the importance of membranes, much effort has been spent to gain a detailed knowledge of their structure in a great variety of cells. It is the purpose of this chapter to review the evidence accumulated up to now concerning the ultrastructure of intracellular and plasma membranes in pancreatic islet cells. The normal architecture of the membrane will be reviewed first, followed by the alterations of this structure during normal or pathological functioning of the islet cells. An earlier review of the subject has been published.1


Tight Junction Islet Cell Pancreatic Islet Secretory Granule Membrane System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Orci, L.: Diabetologia, 10: 163, 1974.PubMedCrossRefGoogle Scholar
  2. 2.
    Robertson, J. D.: Biochem. Soc. Symp., 16: 3, 1959.PubMedGoogle Scholar
  3. 3.
    Dauwalder, M., Whaley, W. G., and Kephart, J. E.: Subcell. Biochem., 1: 225, 1972.Google Scholar
  4. 4.
    Orci, L., Ravazzola, M., Amherdt, M., and Malaisse-Lagae, F.: In: Proceedings of the Eighth Congress of the International Diabetes Federation. Edited by W. J. Malaisse, and J. Pirart. Excerpta Medica, Amsterdam, 1974, p. 104.Google Scholar
  5. 5.
    Rambourg, A.: Int. Rev. Cytol., 31: 57, 1971.PubMedCrossRefGoogle Scholar
  6. 6.
    Nicolson, G. L.: Biochim. Biophys. Acta, 457: 57, 1976.PubMedGoogle Scholar
  7. 7.
    Lazarides, E., and Weber, K.: Proc. Nat. Acad. Sci., 71: 2268, 1974.PubMedCrossRefGoogle Scholar
  8. 8.
    Wessels, N. K., Spooner, B. S., and Luduena, M. A.: In: Locomotion of Tissue Cells. Ciba Foundation 14. Excerpta Medica, Amsterdam, 1973, p. 53.Google Scholar
  9. 9.
    Wessels, N. K., Spooner, B. S., Ash, J. F., Bradley, M. D., Luduena, M. A., Taylor, E. L., Wrenn, J. T., and Yamada, K. M.: Science, 171: 135, 1971.CrossRefGoogle Scholar
  10. 10.
    Painter, R. G., Sheetz, M., and Singer, S. J.: Proc. Nat. Acad. Sci., 72: 1359, 1975.PubMedCrossRefGoogle Scholar
  11. 11.
    Yahara, I., and Edelman, G. M.: Exp. Cell Res., 91: 125, 1975.PubMedCrossRefGoogle Scholar
  12. 12.
    Revel, J. P., and Ito, S.: In: The Specificity of Cell Surface. Edited by E. D. Davis and L. Warren. Prentice Hall, New Jersey, 1967, p. 213.Google Scholar
  13. 13.
    McNutt, N. S., and Weinstein, R. S.: Prog. Biophys. Mol. Biol., 26: 45, 1973.PubMedCrossRefGoogle Scholar
  14. 14.
    Steere, R. L.: J. Biophys. Biochem. Cytol., 3: 45, 1957.PubMedCrossRefGoogle Scholar
  15. 15.
    Moor, H., and Mühlethaler, K.: J. Cell Biol., 17: 609, 1963.PubMedCrossRefGoogle Scholar
  16. 16.
    Branton, D.: Proc. Nat. Acad. Sci., 55: 1048, 1966.PubMedCrossRefGoogle Scholar
  17. 17.
    Branton, D.: Phil. Trans. Roy. Soc. Lond. B., 261: 133, 1971.CrossRefGoogle Scholar
  18. 18.
    Branton, D., Bullivant, S., Gilula, N. B., Karnovsky, M. J., Moor, H., Mühlethaler, K., Northcote, D. H., Packer, L., Satir, B., Satir, P., Speth, V., Staehelin, L. A., Steere, R. L., and Weinstein, R. S.: Science, 190: 54, 1975.PubMedCrossRefGoogle Scholar
  19. 19.
    McNutt, N. S., and Weinstein, R. S.: J. Cell Biol., 47: 666, 1970.PubMedCrossRefGoogle Scholar
  20. 20.
    Ferner, H.: In: Das Inselsystem des Pankreas. G. Thieme, Stuttgart, 1952, p. 119.Google Scholar
  21. 21.
    Branton, D.: Ann. Rev. Plant Physiol. 20: 209, 1969.CrossRefGoogle Scholar
  22. 22.
    Singer, S. J., and Nicolson, G. L.: Science, 175: 720, 1972.PubMedCrossRefGoogle Scholar
  23. 23.
    Singer, S. J.: Ann. Rev. Biochem., 43: 805, 1974.PubMedCrossRefGoogle Scholar
  24. 24.
    Satir, P., and Satir, B.: Exp. Cell Res., 89: 404, 1974.PubMedCrossRefGoogle Scholar
  25. 25.
    Dempsey, G. P., Bullivant, S., and Watkins, W. B.: Science, 179: 190, 1973.PubMedCrossRefGoogle Scholar
  26. 26.
    Montesano, R., Friend, D. S., Perrelet, A., and Orci, L.: J. Cell Biol., 67: 310, 1975.PubMedCrossRefGoogle Scholar
  27. 27.
    Friend, D. S., and Gilula, N. B.: J. Cell Biol., 53: 758, 1972.PubMedCrossRefGoogle Scholar
  28. 28.
    Claude, P., and Goodenough, D. A.: J. Cell Biol., 58: 390, 1973.PubMedCrossRefGoogle Scholar
  29. 29.
    Pricam, C., Humbert, F., Perrelet, A., and Orci, L.: Lab. Invest., 30: 286, 1974.PubMedGoogle Scholar
  30. 30.
    Payton, B. W., Bennett, M. V. L., and Pappas, G. D.: Science, 166: 1641, 1969.PubMedCrossRefGoogle Scholar
  31. 31.
    Gilula, N. B., Reeves, R. O., and Steinbach, A.: Nature (London), 235: 262, 1972.CrossRefGoogle Scholar
  32. 32.
    Orci, L., Perrelet, A., Malaisse-Lagae, F., and Vassalli, P.: C. R. Acad. Sci. Paris, 283: 1509, 1976.Google Scholar
  33. 33.
    Solomon, A. K.: J. Gen. Physiol., 51: 335s, 1968.Google Scholar
  34. 34.
    Orci, L., Malaisse-Lagae, F., Ravazzola, M., Rouiller, D., Renold, A. E., Perrelet, A., and Unger, R. H.: J. Clin. Invest., 56: 1066, 1975.PubMedCrossRefGoogle Scholar
  35. 35.
    Nicolson, G. L., and Singer, S. J.: Proc. Nat. Acad. Sci., 68: 942, 1972.CrossRefGoogle Scholar
  36. 36.
    Sharon, N., and Lis, H.: Science, 177: 949, 1972.PubMedCrossRefGoogle Scholar
  37. 37.
    Smith, S. B., and Revel, J. P.: Elev. Biol., 27: 434, 1972.Google Scholar
  38. 38.
    Orci, L., Carpentier, J.-L., and Roth, J.: Unpublished observations.Google Scholar
  39. 39.
    Orci, L., Rufener, C., Malaisse-Lagae, F., Blondel, B., Amherdt, M., Bataille, D., Freychet, P., and Perrelet, A.: Israel J. Med. Sci., 11: 639, 1975.PubMedGoogle Scholar
  40. 40.
    Orci, L.: In: Peptides Hormones: Molecular and Cellular Aspects. Ciba Foundation Symposium 41. Edited by R. Porter and D. W. Fitzsimons, Excerpts Medica, Amsterdam, 1975, P. 267.Google Scholar
  41. 41.
    Rosenblith, J. Z., Ukena, T. E., Yin, H. H., Berlin, R. D., and Karnovsky, M. J.: Proc. Nat. Acad. Sci., 70: 1625, 1973.PubMedCrossRefGoogle Scholar
  42. 42.
    Taylor, R. B., Duffus, W. P. H., Raff, M. C., and De Petris, S.: Nature (New Biol.), 233: 225, 1971.CrossRefGoogle Scholar
  43. 43.
    Bretscher, M. S., and Raff, M. C.: Nature (London), 258: 43, 1975.CrossRefGoogle Scholar
  44. 44.
    Weibel, E. R.: Int. Rev. Cytol., 26: 235, 1969.PubMedCrossRefGoogle Scholar
  45. 45.
    Orci, L., Amherdt, M., Malaisse-Lagae, F., Rouiller, C., and Renold, A. E.: Science, 179: 82, 1973.PubMedCrossRefGoogle Scholar
  46. 46.
    Orci, L., Perrelet, A., and Friend, D. S.: J. Cell Biol., 1977 (in press).Google Scholar
  47. 47.
    Satir, B., Schooley, L., and Satir, P.: J. Cell Biol., 56: 153, 1973.PubMedCrossRefGoogle Scholar
  48. 48.
    Orci, L., Malaisse-Lagae, F., Ravazzola, M., Amherdt, M., and Renold, A. E.: Science, 181: 561, 1973.PubMedCrossRefGoogle Scholar
  49. 49.
    Orci, L., Amherdt, M., Malaisse-Lagae, F., Perrelet, A., Dulin, W. E., Gerritsen, G. C., Malaisse, W. J., and Renold, A. E.: Diabetologia, 10: 529, 1974.PubMedCrossRefGoogle Scholar
  50. 50.
    Orci, L., Amherdt, M., Malaisse-Lagae, F., Ravazzola, M., Malaisse, W. J., Perrelet, A., and Renold, A. E.: Lab Invest., 34: 451, 1976.PubMedGoogle Scholar
  51. 51.
    Orci, L., Amherdt, M., Henquin, J. C., Lambert, A. E., Unger, R. H., and Renold, A. E.: Science, 180: 647, 1973.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Lelio Orci
    • 1
  • Alain Perrelet
    • 1
  1. 1.Institute of Histology, Medical SchoolUniversity of GenevaGenevaSwitzerland

Personalised recommendations