Junctional Permeability and Its Consequences

  • John D. Pitts
  • Malcolm E. Finbow

Abstract

For the last 100 years attention has been focused on the cell as the unit of life, and indeed this important concept has formed the basis for much of our understanding of structure and function in biological systems. Although this concept is meaningful and accurate for prokaryotes and other unicellular organisms, the cell in a highly developed multicellular organism must lose much of its independence in the milieu of the composite tissues. We must now accept that multicellular organisms are populations of interacting and interdependent cells with properties which cannot be assigned specifically to individual units but instead must be collectively assigned to all the different cells which together are the developing embryo or the adult tissue.

Keywords

Mutant Cell Donor Cell Recipient Cell Intercellular Junction Purine Nucleotide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bennett, M. V. L., 1973, Function of electrotonic junctions in embryonic and adult tissues.Fed. Proc. 32:65.PubMedGoogle Scholar
  2. Bennett, M. V. L., and Dunham, P. B., 1970, Sucrose permeability of junctional membrane at electrotonic synapse, Biophys. J. 10:114a.Google Scholar
  3. Bennett, M. V. L., Dunham, P. B., and Pappas, G. D., 1967, Ion fluxes through a “tight” junction, J. Gen. Physiol. 50:1094.CrossRefGoogle Scholar
  4. Bürk, R. R., Pitts, J. D., and Subak-Sharpe, J. H., 1968, Exchange between hamster cells in culture, Exp. Cell Res. 53:297.PubMedCrossRefGoogle Scholar
  5. Caveney, S., 1974, Intercellular communication in a positional field: Movement of small ions between insect epidermal cells, Dev. Biol. 40:311.PubMedCrossRefGoogle Scholar
  6. Cox, R. P., Krauss, M. R., Balis, M. E., and Daneis, J., 1970, Evidence for transfer of enzyme product as the basis of metabolic cooperation between tissue culture fibroblasts of Lesch-Nyhan disease and normal cells, Proc. Nat. Acad. Sci U.S.A. 67:1573.CrossRefGoogle Scholar
  7. Cox, R. P., Krauss, M. J., Balis, M. E., and Daneis, J., 1972, Communication between normal and enzyme deficient cells in tissue culture. Exp. Cell. Res. 74:251.PubMedCrossRefGoogle Scholar
  8. Cox, R. P., Krauss, M. R., Balis, M. E., and Daneis, J., 1974, Metabolic cooperation in cell culture: Studies of the mechanism of cell interaction, J. Cell. Physiol. 84:237.CrossRefGoogle Scholar
  9. Crick, F. H. C., 1970, Diffusion in embryogenesis, Nature (London) 225:420.CrossRefGoogle Scholar
  10. Crick, F. H. C., and Lawrence, P. A., 1975, Compartments and polyclones in insect development, Science 189:340.PubMedCrossRefGoogle Scholar
  11. Franks, L. M., Riddle, P. N., Carbonell, A. W., and Gey, G. O., 1970, A comparative study of the ultrastructure and lack of growth capacity of adult human prostrate epithelium mechanically separated from its stroma, J. Pathol. 100:113.PubMedCrossRefGoogle Scholar
  12. Fried, M., and Pitts, J. D., 1968, Replication of polyoma virus DNA. 1. A resting cell system for biochemical studies on polyoma virus, Virology 34:761.PubMedCrossRefGoogle Scholar
  13. Fujimoto, W. Y., Subak-Sharpe, J. H., and Seegmiller, J. E., 1971, Hypoxanthine-guanine phosphoribosyltransferase deficiency: Chemical agents selective for mutant or normal cultured fibroblasts in mixed and heterozygote cultures, Proc. Nat. Acad. Sci. U.S.A. 68:1516.CrossRefGoogle Scholar
  14. Garcia-Bellido, A., 1975, Genetic control of wing disc development in Drosophila, in: CibaFoundation Symposium on Cell Patterning, pp. 161–182, Elsevier, Amsterdam.Google Scholar
  15. Garrod, D. R., and Steinberg, M. S., 1973, Tissue-specific sorting-out in two dimensions in relation to contact inhibition of cell movement, Nature (London) 244:568.CrossRefGoogle Scholar
  16. Gilula, N. B., 1974, Junctions between cells, in: Cell Communication (R. P. Cox, ed.), pp. 1–29, Wiley, New York.Google Scholar
  17. Gilula, N. B., Reeves, O. R., and Steinbach, A., 1972, Metabolic coupling, ionic coupling and cell contacts, Nature (London)235:262.CrossRefGoogle Scholar
  18. Goldfarb, P. S. G., Slack, C., Subak-Sharpe, J. H., and Wright, E. D., 1975, Transport at the cellular level, in: Society of Experimental Biology Symposium 28 (M. A. Sleigh and D. H. Jennings, eds.), pp. 463–484, Cambridge University Press, Cambridge.Google Scholar
  19. Grainger, R. M., and Wessells, N. K., 1974, Does RNA pass from mesenchyme to epithelium during an embryonic tissue interaction? Proc. Nat. Acad. Sci. U.S.A. 71:4747.CrossRefGoogle Scholar
  20. Greaves, M. F., 1975, Cellular Recognition, Chapman and Hall, London.Google Scholar
  21. Johnson, R. G., and Sheridan, J. D., 1971, Junctions between cancer cells in culture: Ultrastructure and permeability, Science 174:717.PubMedCrossRefGoogle Scholar
  22. Kelley, R. O., 1968, An electron microscope study of chordamesoderm-neuroectoderm association in gastrulae of a toad Xenopus laevis, J. Exp. Zool. 172:153.CrossRefGoogle Scholar
  23. Kolodny, G. M., 1971, Evidence for the transfer of macromolecular RNA between mammalian cells in culture, Exp. Cell Res. 65:313.PubMedCrossRefGoogle Scholar
  24. Kolodny, G. M., 1973, Transfer of cytoplasmic and nuclear proteins between cells in culture, J. Mol. Biol. 78:197.PubMedCrossRefGoogle Scholar
  25. Ley, K. D., 1975, Detection of G1 proteins in cells synchronized by isoleucine deprivation or mitotic selection, J. Cell Biol. 66:95.PubMedCrossRefGoogle Scholar
  26. Mintz, B., and Baker, W. W., 1967, Normal mammalian muscle differentiation and gene control of isocitrate dehydrogenase synthesis, Proc. Nat. Acad. Sci. U.S.A. 58:592.CrossRefGoogle Scholar
  27. Pitts, J. D., 1971, Molecular exchange and growth control in tissue culture, in: Ciba Foundation Symposium on Growth Control in Cell Cultures (G. E. W. Wolstenholme and J. Knight, eds.), pp. 89–105, Churchill Livingstone, London.Google Scholar
  28. Pitts, J. D., 1972, Direct interaction between animal cells, in:Third Lepetit Colloquium on CellInteractions (L. G. Silvestri, ed.), pp. 277–285, North-Holland Publ., Amsterdam.Google Scholar
  29. Pitts, J. D., 1976, Junctions as channels of direct communication between cells, in: TheDevelopmental Biology of Plants and Animals (C. F. Graham and E. F. Wareing, eds.), pp. 96–110, Blackwell, Oxford.Google Scholar
  30. Pitts, J. D., and Simms, J. W., 1976, Permeability of junctions between animal cells: Intercellular transfer of nucleotide but not RNA, Exp. Cell Res. (in press).Google Scholar
  31. Sheridan, J. D., 1974, Low resistance junctions: Some functional considerations, in: The CellSurface in Development (A. A. Moscana, ed.), pp. 187–206, Wiley, New York.Google Scholar
  32. Sheridan, J. D., Finbow, M. E., and Pitts, J. D., 1975, Metabolic cooperation in culture: Possible involvement of junctional transfer in regulation of enzyme activities, J. CellBiol. 67:396a.Google Scholar
  33. Simms, J. W., 1973, Interactions between mammalian cells in tissue culture, Ph.D. Thesis, University of Glasgow, Scotland.Google Scholar
  34. Steinberg, M. S., and Garrod, D. R., 1975, Observations on the sorting-out of embryonic cells in monolayer culture, J. Cell Sci. 18:385.PubMedGoogle Scholar
  35. Subak-Sharpe, J. H., Biirk, R. R., and Pitts, J. D., 1966, Metabolic co-operation by cell transfer between genetically different mammalian cells in tissue culture, Heredity 21:342.Google Scholar
  36. Subak-Sharpe, J. H., Biirk, R. R., and Pitts, J. D., 1969, Metabolic co-operation between biochemically marked cells in tissue culture, J. Cell Sci. 4:353.PubMedGoogle Scholar
  37. Warner, A. E., and Lawrence, P. A., 1973, Electrical coupling across developmental boundaries in insect epidermis, Nature (London) 245:47.CrossRefGoogle Scholar
  38. Wartiovaara, J., Lehtonen, E., Nordling, S., and Saxen, L., 1972, Do membrane filters prevent cell contacts? Nature (London) 238:407.CrossRefGoogle Scholar
  39. Wolpert, L., 1969, Positional information and the spatial pattern of cellular differentiation, J. Theor. Biol. 25:1.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • John D. Pitts
    • 1
  • Malcolm E. Finbow
    • 1
  1. 1.Department of BiochemistryUniversity of GlasgowGlasgowScotland

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