Isolation and Characterization of Low Density Lipoproteins

  • Diana M. Lee


Since the first isolation of lipoproteins by Macheboeuf1 in 1929, little progress was made in the investigation of lipoproteins until Pedersen2 found that the so-called “X protein” in human serum had a low hydrated density. Using this “low density” property, he isolated the “X protein” by ultracentrifugal flotation and determined its hydrated density to be 1.03–1.04 g/ml. This finding, and the use of the flotation technique, marked the beginning of a systematic ultracentrifugal study of low density lipoproteins.3–6 Gofman et al.4 suggested in 1950 that the solvent density of serum (excluding the density contributed by proteins or lipoproteins) be raised by the addition of NaCl to 1.063 g/ml to float the lipoproteins of hydrated density 1.04 g/ml or less by ultracentrifugation. Therefore, lipoproteins floating at d 1.063 g/ml came to be known as low density lipoproteins and lipoproteins sedimenting at d 1.063 g/ml were referred to as high density lipoproteins. The term, flotation coefficient, S f , was introduced by Gofman and coworkers4 as the negative sedimentation coefficient in a sodium chloride solution of d = 1.063 g/ml at 26°C.


Dextran Sulfate Association Complex Protein Moiety Solvent Density Normal Human Plasma 


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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Diana M. Lee
    • 1
  1. 1.The Cardiovascular Research ProgramOklahoma Medical Research FoundationOklahoma CityUSA

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