Advertisement

Biochemical Studies of Synaptic Macromolecules

Are There Specific Synaptic Components?
  • I. G. Morgan
  • G. Gombos
Part of the Current Topics in Neurobiology book series (CTNB)

Abstract

It is highly likely that the relevant specificities of the nerve ending region are localized in the synaptic plasma membrane since it is presumably contact between the external surfaces of membranes which is important in neuronal recognition. But even with this initial precision several levels of specificity have to be considered. The specificities of the synaptosomal plasma membrane relative to the plasma membranes of nonneural cells, to those of nonneuronal neural cells, and to other parts of the neuronal plasma membrane such as the dendritic, perikaryal, and axonal plasma membranes must be analyzed. And within the synaptic region the synaptosomal plasma membrane consists of non-junctional and junctional regions, the latter consisting of pre- and postsynaptic membranes.

Keywords

Synaptic Vesicle Synaptosomal Membrane Synaptic Plasma Membrane Synaptosomal Fraction Synaptic Junction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abe, T., and Norton, W. T., 1974, The characterization of sphingolipids from neurons and astroglia of immature rat brain, J. Neurochem. 23: 1025.Google Scholar
  2. Akeson, R., and Herschman, H., 1974, Neural antigens of morphologically differentiated neuroblastoma cells, Nature (London) 249: 620.Google Scholar
  3. Arbogast, B. W., and Arsenis, C., 1974, The enzymatic ontogeny of neurons and glial cells isolated from postnatal rat cerebral gray matter, Neurobiology 4: 21.Google Scholar
  4. Avrova, N. F., Chenykaeva, E. Y., and Obukhova, E. L., 1973, Ganglioside composition and content of rat-brain subcellular fractions, J. Neurochem. 20: 997.Google Scholar
  5. Banker, G., Crain, B., and Cotman, C. W., 1972, Molecular weights of the polypeptide chains of synaptic plasma membranes, Brain Res. 42: 508.Google Scholar
  6. Banker, G., Churchill, L., and Cotman, C. W., 1974, Proteins of the postsynaptic density J. Cell Biol. 63: 456.Google Scholar
  7. Barnola, F. V., Villegas, R., and Camejo, G., 1973, Tetrododoxin receptors in plasma membranes isolated from lobster nerve fibers, Biochim. Biophys. Acta 298: 84.Google Scholar
  8. Barondes, S. H., 1968, Incorporation of radioactive glucosamine into macromolecules at nerve endings, J. Neurochem. 15: 699.Google Scholar
  9. Barondes, S. H., 1974, Synaptic macromolecules: Identification and metabolism, Annu. Rev. Biochem. 43: 147.Google Scholar
  10. Bittiger, H., and Schnebli, H. P., 1974, Binding of concanavalin A and ricin to synaptic junctions of rat brain, Nature (London) 249: 370.Google Scholar
  11. Blomstrand, C., and Hamberger, A., 1969, Protein turnover in cell-enriched fractions from rabbit brain, J. Neurochem. 16: 1401.Google Scholar
  12. Bloom, F. E., and Aghajanian, G. K., 1966, Cytochemistry of synapses: Selective staining for electron microscopy, Science 154: 1575.Google Scholar
  13. Bloom, F. E., and Aghajanian, G. K., 1968, Fine structural and cytochemical analysis of the staining of synaptic junctions with phosphotungstic acid, J. Ultrastruct. Res. 22: 361.Google Scholar
  14. Bock, E., and Jorgensen, O. S., 1975, Rat brain synaptic vesicles and synaptic plasma membranes compared by crossed immunoelectrophoresis, FEBS Lett. 52: 37.Google Scholar
  15. Bock, E., Jorgensen, O. S., and Morris, S. J., 1974, Antigen—antibody crossed electrophoresis of rat brain synaptosomes and synaptic vesicles: Correlation to water-soluble antigens from rat brain, J. Neurochem. 22: 1013.Google Scholar
  16. Bondareff, W., and Sjöstrand, J., 1969, Cytochemistry of synaptosomes, Exp. Neurol. 24: 450.Google Scholar
  17. Bosmann, H. B., 1971, Identification of proteins and glycoproteins synthesized by isolated rat cortex synaptosomes in vitro, Neurobiology 1: 144.Google Scholar
  18. Bosmann, H. B., 1972, Sialic acid on the synaptosome surface and the effect of concana- valin A and trypsin on synaptosome electrophoretic mobility, FEBS Lett. 22: 97.Google Scholar
  19. Bosmann, H. B., and Carlson, W., 1972, Identification of sialic acid at the nerve-ending periphery and electrophoretic mobility of isolated synaptosomes, Exp. Cell Res. 72: 436.Google Scholar
  20. Brady, R. O., and Quarles, R. H., 1973, The enzymology of myelination, Mol. Cell Biochem. 2: 23.Google Scholar
  21. Breckenridge, W. C., and Morgan, I. G., 1972, Common glycoproteins of synaptic vesicles and the synaptosomal plasma membrane, FEBS Lett. 22: 253.Google Scholar
  22. Breckenridge, W. C., Gombos, G., and Morgan, I. G., 1972, The lipid composition of adult rat brain synaptosomal plasma membranes, Biochim. Biophys. Acta 266: 695.Google Scholar
  23. Broquet, P., and Louisot, P., 1971, Biosynthèse des glycoprotéines cérébrales. II. Localisation subcellulaire des transglycosylases cérébrales, Biochimie 53: 921.Google Scholar
  24. Camejo, G., Villegas, G. M., Barnola, F. V., and Villegas, R., 1969, Characterization of two different membrane fractions isolated from the first stellar nerve of the squid Dosidicus gigas, Biochim. Biophys. Acta 193: 247.Google Scholar
  25. Chacko, G. K., Goldman, D. E., and Pennock, B. E., 1972, Composition and characterization of the lipids of garfish (Lepisosteus osseus) olfactory nerve: A tissue rich in axonal membranes, Biochim. Biophys. Acta 280: 1.Google Scholar
  26. Chacko, G. K., Goldman, D. E., Malhotra-H., C., and Dewey, M. M., 1974, Isolation and characterization of plasma membrane fractions from garfish (Lepisosteus osseus) olfactory nerve, J. Cell Biol. 62: 831.Google Scholar
  27. Cohen, J., Dutton, G. R., Wilkin, G. P., Wilson, J. E., and Balazs, R., 1974, A preparation of viable cell perikarya from developing rat cerebellum with preservation of a high degree of morphological integrity, J. Neurochem. 23: 899.Google Scholar
  28. Costin, A., Cotman, C., Hafemann, D. R., and Herschman, H. R., 1972, Effect of anti-brain synaptosomal fraction serum and complement on evoked potentials and impedance, Experientia 28: 411.Google Scholar
  29. Cotman, C. W., and Matthews, D. A., 1971, Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterization, Biochim. Biophys. Acta 249: 380.Google Scholar
  30. Cotman, C. W., and Taylor, D., 1972, Isolation and structural studies on synapticGoogle Scholar
  31. complexes from rat brain, J. Cell Biol. 55:696.Google Scholar
  32. Cotman, C. W., and Taylor, D., 1974, Localization and characterization of concanavalin A receptors in the synaptic cleft, J. Cell Biol. 62: 236.Google Scholar
  33. Cotman, C. W., Blank, M. L., Moehl, A., and Snyder, F., 1969, Lipid composition of synaptic plasma membranes isolated from rat brain by zonal centrifugation, Biochemistry 8: 4606.Google Scholar
  34. Cotman, C. W., McCaman, R. E., and Dewhurst, S. A., 1971, Subsynaptosomal distribution of enzymes involved in the metabolism of lipids, Biochim. Biophys. Acta 249: 395.Google Scholar
  35. Cotman, C. W., Banker, G., Churchill, L., and Taylor, D., 1974, Isolation of post-synaptic densities from rat brain, J. Cell Biol. 63: 441.Google Scholar
  36. Davis, G. A., and Bloom, F. E., 1970, Proteins of synaptic junctional complexes, J. Cell Biol. 47: 46A.Google Scholar
  37. Davis, G. A., and Bloom, F. E., 1973, Isolation of junctional complexes from rat brain, Brain Res. 62: 135.Google Scholar
  38. Dekirmenjian, H., Brunngraber, E. G., Lemkey-Johnston, N., and Larramendi, L. M. H., 1969, Distribution of gangliosides, glycoprotein-NANA and acetylcholinesterase in axonal and synaptosomal fractions of cat cerebellum, Exp. Brain Res. 8: 97.Google Scholar
  39. Den, H., and Kaufman, B., 1968, Ganglioside and glycoprotein glycosyltransferases in synaptosomes, Fed. Proc. 27: 346.Google Scholar
  40. Den, H., Kaufman, B., and Roseman, S., 1970, Properties of some glycosyltransferases in embryonic chicken brain, J. Biol. Chem. 245: 6607.Google Scholar
  41. Denburg, J. L., 1972, An axon plasma membrane preparation from the walking legs of the lobster Hornarus americanus, Biochim. Biophys. Acta 282: 453.Google Scholar
  42. De Robertis, E., and Rodriguez de Lores Amaiz, G., 1969, Structural components of the synaptic region, in: Handbook of Neurochemistry, Vol. II ( A. Lajtha, ed.), pp. 365–392, Plenum Press, New York.Google Scholar
  43. De Robertis, E., Lapetina, E., Pecci Saavedra, J., and Soto, E. F., 1966, In vivo and in vitro action of antisera against isolated nerve-endings of the brain cortex, Life Sci. 5: 1979.Google Scholar
  44. De Robertis, E., Lapetina, E. G., and Wald, F., 1968, The effect of antiserum against nerve-ending membranes from cat cerebral cortex on the ultrastructure of isolated nerve-endings and mollusc neurons, Exp. Neurol. 21: 322.Google Scholar
  45. Derry, D. M., and Wolfe, L. S., 1967, Gangliosides in isolated neurons and glial cells, Science 158: 1450.Google Scholar
  46. de Vries, G. H., and Barondes, S. H., 1971, Incorporation of “C-N-acetylneuraminic acid into brain glycoproteins and gangliosides in vivo, J. Neurochem. 18: 101.Google Scholar
  47. de Vries, G. H., and Norton, W. T., 1974, The lipid composition of axons from bovine brain, J. Neurochem. 22: 259.Google Scholar
  48. de Vries, G. H., Norton, W. T., and Raine, C. S., 1972, Axons: Isolation from mammalian central nervous system, Science 175: 1370.Google Scholar
  49. Dutton, G. R., Haywood, P., and Barondes, S. H., 1973, Carbon-14 labeled glucosamine incorporation into specific products in the nerve-ending fraction in vivo and in vitro, Brain Res. 57: 397.Google Scholar
  50. Feit, H., and Barondes, S. H., 1970, Colchicine-binding activity in particulate fractions of mouse brain, J. Neurochem. 17: 1355.Google Scholar
  51. Feit, H., Dutton, G. R., Barondes, S. H., and Shelanski, M. L., 1971, Microtubule protein: Identification and transport to nerve-endings, J. Cell Biol. 51: 138.Google Scholar
  52. Festoff, B. W., Appel, S. H., and Day, E., 1971, Incorporation of 14C-glucosamine into synaptosomes in vitro, J. Neurochem. 18: 1871.Google Scholar
  53. Fischer, S., Cellino, M., Zambrano, F., Zampighi, G., Tellez Nagel, M., Marcus, D., and Canessa-Fischer, M., 1970, The molecular organization of nerve membranes. I. Isolation and characterization of plasma membranes from the retinal axons of the squid: an axolemma rich preparation, Arch. Biochem. Biophys. 138: 1.Google Scholar
  54. Freysz, J., Bieth, R., Judes, C., Sensenbrenner, M., Jacob, M., and Mandel, P., 1968, Quantitative distribution of phospholipids in neurons and glial cells isolated from rat cerebral cortex, J. Neurochem. 15: 307.Google Scholar
  55. Gombos, G., Hermetet, J. C., Reeber, A., Zanetta, J. P., and Treska-Ciesielski, J., 1972, The composition of glycopeptides, derived from neural membranes which affect neurite growth in vitro, FEBS Lett. 24: 247.Google Scholar
  56. Gombos, G., Reeber, A., Zanetta, J. P., and Vincendon, G., 1974a, Fractionation of nervous tissue membrane glycoproteins, in: Actes du Colloque International No. 221 du CNRS sur les Glycoconjugués, pp. 829–844, Villeneuve d’Ascq, 20–27 June 1973, Editions du CNRS, Paris.Google Scholar
  57. Gombos, G., Zanetta, J. P., Reeber, A., Morgan, I. G., and Vincendon, G., 1974b, Affinity chromatography of brain membrane glycoproteins, Biochem. Soc. Trans. 2: 627.Google Scholar
  58. Grefrath, S. P., and Reynolds, J. A., 1973, Polypeptide components of an excitable plasma membrane, J. Biol. Chem. 248: 6091.Google Scholar
  59. Gurd, J. W., and Mahler, H. R., 1974, Fractionation of synaptic plasma membrane glycoproteins by lectin affinity chromatography, Biochemistry 13: 5193.Google Scholar
  60. Gurd, J. W., Jones, L. R., Mahler, H. R., and Moore, W. J., 1974, Isolation and partialGoogle Scholar
  61. characterization of rat brain synaptic plasma membranes, J. Neurochem. 22:281.Google Scholar
  62. Hamberger, A., and Svennerholm, L., 1971, Composition of gangliosides and phospho-lipids of neuronal and glial cell enriched fractions, J. Neurochem. 18: 1821.Google Scholar
  63. Hemminki, K., 1973, Plasma membranes isolated from immature brain cells, Commentat. Biol. Soc. Fenn. 70: 1.Google Scholar
  64. Hemminki, K., Hemminki, E., and Giacobini, E., 1973, Activity of enzymes related to neurotransmission in neuronal and glial fractions, Int. J. Neurosci. 5: 87.Google Scholar
  65. Henn, F. A., Hansson, H. A., and Hamberger, A., 1972, Preparation of plasma membrane from isolated neurons, J. Cell Biol. 533: 654.Google Scholar
  66. Herschman, H. R., Cotman, C. W., and Matthews, D. H. 1972, Serologic specificities of brain subcellular organelles. I. Antisera to synaptosomal fractions, J. Immunol. 108: 1362.Google Scholar
  67. Jarosch, E., and Precht, W., 1972, Effects of antibodies directed toward membrane fragments of synaptosomes on cerebellar field potentials, Brain Res. 42: 225.Google Scholar
  68. Jeffrey, P. L., and Austin, L., 1973, Axoplasmic transport, Prog. Neurobiol. 2: 205.Google Scholar
  69. Jones, D. H., and Matus, A. I., 1974, Isolation of synaptic plasma membrane from brain by combined flotation—sedimentation density gradient centrifugation, Biochim. Biophys. Acta 356: 276.Google Scholar
  70. Jorgensen, O. S., and Bock, E., 1974, Brain specific synaptosomal membrane proteins demonstrated by crossed immunoelectrophoresis, J. Neurochem. 23: 879.Google Scholar
  71. Karlsson, J. O., Hamberger, A., and Henn, F. A., 1973, Polypeptide composition of membranes derived from neuronal and glial cells, Biochim. Biophys. Acta 298: 219.Google Scholar
  72. Ko, G. K. W., and Raghupathy, E., 1971, Glycoprotein biosynthesis in the developing rat brain. I. Microsomal galactosyltransferase utilizing endogenous and exogenous protein acceptors, Biochim. Biophys. Acta 244: 396.Google Scholar
  73. Ko, G. K. W., and Raghupathy, E., 1972, Glycoprotein biosynthesis in the developing rat brain. II. Microsomal galactosaminyltransferase utilizing endogenous and exogenous protein acceptors, Biochim. Biophys. Acta 264: 129.Google Scholar
  74. Kornguth, S. E., Anderson, J. W., and Scott, G., 1969, Isolation of synaptic complexes in a caesium chloride density gradient: Electron microscopic and immunohistochemical studies, J. Neurochem. 16: 1017.Google Scholar
  75. Lemkey-Johnston, N., and Dekirmenjian, H., 1970, The identification of fractions enriched in nonmyelinated axons from rat whole brain, Exp. Brain Res. 11: 392.Google Scholar
  76. Lemkey-Johnston, N., and Larramendi, L. M. H., 1968, The separation and identification of fractions of non-myelinated axons from the cerebellum of the cat, Exp. Brain Res. 5: 326.Google Scholar
  77. Levitan, I. B., Mushynski, W. E., and Ramirez, G., 1972, Highly purified synaptosomal membranes from rat brain: Preparation and characterization, J. Biol. Chem. 247: 5376.Google Scholar
  78. Lim, R., and Hsu, L. W., 1971, Studies on brain-specific membrane proteins, Biochim. Biophys. Acta 249: 569.Google Scholar
  79. Livett, B. G., Rostas, J. A. P., Jeffrey, P. L., and Austin, L., 1974a, Antigenicity of isolated synaptosomal membranes, Exp. Neurol. 43: 330.Google Scholar
  80. Livett, B. G., Fenwick, E. M., and Eadie, J., 1974b, Ca“-dependent redistribution of antigenic components during membrane fusion, Proc. Aust. Biochem. Soc. 7: 57.Google Scholar
  81. Marcus, D., Canessa-Fischer, M., Zampighi, G., and Fischer, S., 1972, The molecular organization of nerve membranes. VI. The separation of axolemma from Schwann cell membranes of giant and retinal squid axons by density gradient centrifugation, J. Membr. Biol. 9: 209.Google Scholar
  82. Margolis, R. U., 1969, Mucopolysaccharides, in: Handbook of Neurochemistry, Vol. I ( A. Lajtha, ed.), pp. 245–260, Plenum Press, New York.Google Scholar
  83. Margolis, R. U., and Margolis, R. K., 1974, Distribution and metabolism of mucopolysaccharides and glycoproteins in neuronal perikarya, astrocytes and oligodendroglia, Biochemistry 13: 2849.Google Scholar
  84. Marinari, U. M., Morgan, I. G., Mack, G., and Gombos, G., 1972, Synthesis of synaptic glycoproteins. II. Delayed labeling of the glycoproteins of synaptic vesicles and synaptosomal plasma membranes, Neurobiology 2: 176.Google Scholar
  85. Matus, A., De Petris, S., and Raff, M. C., 1973, Mobility of concanavalin A receptors in myelin and synaptic membranes, Nature (London) New Biol. 244: 278.Google Scholar
  86. McBride, W. J., and Cohen, H., 1972, Cytochemical localization of acetylcholinesterase on isolated synaptosomes, Brain Res. 41: 489.Google Scholar
  87. McBride, W. J., and Van Tassel, J., 1972, Resolution of proteins from subfractions of nerve-endings, Brain Res. 44: 177.Google Scholar
  88. McBride, W. J., Mahler, H. R., Moore, W. J., and White, F. P., 1970, Isolation and characterization of membranes from rat cerebral cortex, J. Neurobiol. 2: 73.Google Scholar
  89. McMillan, P. N., Mickey, D. D., Kaufman, B., and Day, E. D., 1971, Specificity and cross-reactivity of antimyelin antibodies as determined by sequential adsorption analysis, J. Immunol. 107: 1611.Google Scholar
  90. Medzihradsky, F., Nandhasri, P. S., Idoyaga-Vargas, V., and Sellinger, O. Z., 1971, A comparison of the ATPase activity of the glial cell fraction and the neuronal perikaryal fraction isolated in bulk from rat cerebral cortex, J. Neurochem. 18: 1599.Google Scholar
  91. Mickey, D. D., McMillan, P. N., Appel, S. H., and Day, E. D., 1971, Specificity and cross-reactivity of antisynaptosome antibodies as determined by sequential adsorption analysis, J. Immunol. 107: 1599.Google Scholar
  92. Morgan, I. G., Wolfe, L. S., Mandel, P., and Gombos, G., 1971, Isolation of plasma membranes from rat brain, Biochim. Biophys. Acta 241: 737.Google Scholar
  93. Morgan, I. G., Reith, M., Marinari, U., Breckenridge, W. C., and Gombos, B., 1972, The isolation and characterization of synaptosomal plasma membranes, in: Advan. Exp. Med. Biol. 25: 209.Google Scholar
  94. Morgan, I. G., Breckenridge, W. C., Vincendon, G., and Gombos, G., 1973a, The proteins of nerve-ending membranes, in: Proteins of the Nervous System (Schneider, D. Johnson,ed.), pp. 171–192, Raven Press, New York.Google Scholar
  95. Morgan, I. G., Zanetta, J. P., Breckenridge, W. C., Vincendon, G., and Gombos, G., 1973b, The chemical structure of synaptic membranes, Brain Res. 62: 405.Google Scholar
  96. Morgan, I. G., Marinari, U. M., Dutton, G. R., Vincendon, G., and Gombos, G., 1974, The biosynthesis of synaptic glycoproteins, in: Actes du Colloque International No. 221 du CNRS sur les Glycoconjugués, pp. 1043–1052, Villeneuve d’Ascq, 20–27 June 1973, Editions du CNRS, Paris.Google Scholar
  97. Morgan, I. G., Marinari, U. M., Dutton, G. R., Vincendon, G., and Gombos, G., 1974, The biosynthesis of synaptic glycoproteins, in: Actes du Colloque International No. 221 du CNRS sur les Glycoconjugués, pp. 1043–1052, Villeneuve d’Ascq, 20–27 June 1973, Editions du CNRS, Paris.Google Scholar
  98. Nagata, Y., Mikoshiba, K., and Tsukada, Y., 1974, Neuronal cell body-enriched and glial cell-enriched fractions from young and adult rat brains: Preparations, and morphological and biochemical properties, J. Neurochem. 22: 493.Google Scholar
  99. Norton, W. T., and Poduslo, S. E., 1970, Neuronal soma and whole neuroglia of rat brain: A new isolation technique, Science 167: 1144.Google Scholar
  100. Norton, W. T., and Poduslo, S. E., 1971, Neuronal perikarya and astroglia of rat brain: Chemical composition during myelination, J. Lipid Res. 12: 84.Google Scholar
  101. Orosz, A., Hamori, J., Falus, A., Madarasz, E., Lakos, I., and Adam, G., 1973, Specific antibody fragments against the postsynaptic web, Nature (London) New Biol. 245: 18.Google Scholar
  102. Orosz, A., Madarasz, E., Falus, A., and Adam, G., 1974, Demonstration of detergent-soluble antigen specific for the synaptosomal membrane fraction isolated from the cat cerebral cortex, Brain Res. 76: 119.Google Scholar
  103. Pfenninger, K. H., 1971, The cytochemistry of synaptic densities. I. An analysis of the bismuth iodide impregnation method, J. Ultrastruct. Res. 34: 103.Google Scholar
  104. Poduslo, S. E., and Norton, W. T., 1972, Isolation and some chemical properties of oligodendroglia from calf brain, J. Neurochem. 19: 727.Google Scholar
  105. Pyke, K. W., and Dent, P. B., 1971, The in vitro effects of phytohaemagglutinin (PHA) on human neuroblastoma and chick embryo ganglion cells, Fed. Proc. 30: 398A.Google Scholar
  106. Raghupathy, E., Ko, G. K. W., and Peterson, N. A., 1972, Glycoprotein biosynthesis in the developing rat brain. III. Are glycoprotein glycosyl transferases present in synaptosomes? Biochim. Biophys. Acta 286: 339.Google Scholar
  107. Raiteri, M., Bertollini, A., and la Bella, R., 1972, Synaptosome antisera effect permeabil- ity of synaptosomal membranes in vitro, Nature (London) New Biol. 238: 242.Google Scholar
  108. Rambourg, A., and Leblond, C. P., 1967, Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat, J. Cell Biol. 32: 27.Google Scholar
  109. Ramirez, G., 1974, Nonspecific incorporation of glucosamine into rat brain synaptosomes and endoplasmic reticulum, Biochim. Biophys. Acta 338: 337.Google Scholar
  110. Ramirez, G., Levitan, I. B., and Mushynski, W. E., 1972, Highly purified synaptosomal membranes from rat brain. Incorporation of amino-acids into membrane proteins in vitro, J. Biol. Chem. 247: 5382.Google Scholar
  111. Reith, M., Morgan, I. G., Gombos, G., Breckenridge, W. C., and Vincendon, G., 1972, Synthesis of synaptic glycoproteins. I. The distribution of UDP-galactose: N-acetylglucosamine galactosyl transferase and thiamine diphosphatase in adult rat brain subcellular fractions, Neurobiology 2: 169.Google Scholar
  112. Robert, J., and Rebel, G., 1975, Gangliosides of cultured glial cells, Abstracts Vth Int. Meeting ISN Barcelona, p. 219.Google Scholar
  113. Rodriguez de Lores Amaiz, G., Alberici, M., and De Robertis, E., 1967, Ultrastructural and enzymic studies of cholinergic and non cholinergic synaptic membranes isolated from brain cortex, J. Neurochem. 14: 215.Google Scholar
  114. Rose, S. P. R., 1967, Preparation of enriched fractions from cerebral cortex containing isolated, metabolically active neuronal and glial cells, Biochem. J. 102: 33.Google Scholar
  115. Roseman, S., 1970, The synthesis of complex carbohydrates by multiglycosyl transferase systems and their potential function in intercellular adhesion, Chem: Phys. Lipids 5: 270.Google Scholar
  116. Schachner, M., 1974, NS-1 (nervous system antigen-1), a glial-cell-specific antigenic component of the surface membrane, Proc. Natl. Acad. Sci. USA 71: 1795.Google Scholar
  117. Schengrund, C. L., and Rosenberg, A., 1970, Intracellular location and properties ofGoogle Scholar
  118. bovine brain sialidase, J. Biol. Chem. 245:6196.Google Scholar
  119. Sellinger, O. Z., Borens, R. N., and Nordrum, L. M., 1969, The action of trypsin and neuraminidase on the synaptic membranes of brain cortex, Biochim. Biophys. Acta 173: 185.Google Scholar
  120. Sellinger, O. Z., Azcurra, J. M., Johnson, D. E., Ohlsson, W. G., and Lodin, Z., 1971, Independence of protein synthesis and drug uptake in nerve cell bodies and glial cells isolated by a new technique, Nature (London) New Biol. 230: 253.Google Scholar
  121. Suzuki, K., Poduslo, S. E., and Norton, W. T., 1967, Gangliosides in the myelin fraction of developing rats, Biochim. Biophys. Acta 144: 375.Google Scholar
  122. Tettamanti, G., Morgan, I. G., Gombos, G., Vincendon, G., and Mandel, P., 1972, Subsynaptosomal localization of brain particulate neuraminidase, Brain Res. 47: 516.Google Scholar
  123. Treska-Ciesielski, J., Gombos, G., and Morgan, I. G., 1971, Effet de la concanavaline A sur les neurones de ganglions spinaux d’embryons de poulet en culture, C. R. Acad. Sc. Paris 273D: 1041.Google Scholar
  124. Trading, R., Shelanski, M. L., Daniels, M. P., and Morell, P., 1974, Comparison of surface membranes isolated from cultured murine neuroblastoma cells in the differentiated or undifferentiated state, J. Biol. Chem. 249: 3973.Google Scholar
  125. Ueda, T., Maeno, H., and Greengard, P., 1973, Regulation of endogenous phosphorylation of specific proteins in synaptic membrane fractions from rat brain by adenosine 3’-5’ monophosphate, J. Biol. Chem. 248: 8295.Google Scholar
  126. Uesugi, S., Dulac, N. C., Dixon, J. F., Hexum, T. D., Dahl, J. L., Perue, J. F., and Hokin, L. E., 1971, Studies on the characterization of the sodium-potassium transport adenosine triphosphatase. VI. Large scale partial purification and properties of a lubrol-solubilized bovine brain enzyme, J. Biol. Chem. 246: 531.Google Scholar
  127. van Nieuw Amerongen, A., and Roukema, P. A., 1974, GP-350, a sialoglycoprotein from calf brain: Its subcellular localization and occurrence in various brain areas, J. Neurochem. 23: 85.Google Scholar
  128. Vos, J., Kuriyama, K., and Roberts, E., 1969, Distribution of acid mucopolysaccharides in subcellular fractions of mouse brain, Brain Res. 12: 172.Google Scholar
  129. Waehneldt, T. V., Morgan, I. G., and Gombos, G., 1971, The synaptosomal plasma membrane: Protein and glycoprotein composition, Brain Res. 34: 403.Google Scholar
  130. Wald, F., Mazzuchelli, A. N., Lapetina, E. G., and De Robertis, E., 1968, The effect of antiserum against nerve-ending membranes from cat cerebral cortex on the bioelectrical activity of mollusc neurons, Exp. Neurol. 21: 336.Google Scholar
  131. Walters, B. B., and Matus, A. I., 1975, Proteins of the synaptic junction, Biochem. Soc. Trans. 3: 109.Google Scholar
  132. Whittaker, V. P., 1969, The synaptosome, in: Handbook of Neurochemistry, Vol. II ( A. Lajtha, ed.), pp. 327–364, Plenum Press, New York.Google Scholar
  133. Whittaker, V. P., Michaelson, I. A., and Kirkland, R. J. A., 1964, The separation of synaptic vesicles from nerve ending particles (synaptosomes), Biochem. J. 90: 293.Google Scholar
  134. Zambrano, F., Cellino, M., and Canessa-Fischer, M., 1971, The molecular organization of nerve membranes. IV. The lipid composition of plasma membranes from squid retinal axons, J. Membr. Biol. 6: 289.Google Scholar
  135. Zanetta, J. P., Morgan, I. G., and Gombos, G., 1975, Synaptosomal plasma membrane glycoproteins: Fractionation by affinity chromatography on concanavalin A, Brain Res. 83: 337.Google Scholar
  136. Zatz, M., and Barondes, S. H., 1970, Fucose incorporation into glycoproteins of mouse brain, J. Neurochem. 17: 157.Google Scholar
  137. Zatz, M., and Barondes, S. H., 1971, Particulate and solubilized fucosyl transferases from mouse brain, J. Neurochem. 18: 1625.Google Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • I. G. Morgan
    • 1
  • G. Gombos
    • 1
  1. 1.Chargés de Recherche au Centre National de la Recherche ScientifiqueCentre de Neurochimie du CNRSStrasbourgFrance

Personalised recommendations