The Expression of Neuronal Specificity in Tissue Culture

  • Richard P. Bunge
Part of the Current Topics in Neurobiology book series (CTNB)


The earliest work with nerve tissue in culture was undertaken by experimental embryologists and was designed to address several pressing questions that could not be definitively studied in the developing embryo. Of this early work, the contribution of Harrison (1910) to the understanding of the mechanisms involved in axonal elongation and that of Weiss (reviewed in 1955) to the study of the mechanisms of axon guidance are particularly well known. More recently, the use of tissue culture made a further noteworthy contribution to neuroembryology when employed to demonstrate the humoral nature of the nerve growth factor of the mouse submandibular gland (Levi-Montalcini et al., 1954). Considering the substantial contributions made by these relatively few efforts, it is surprising that culture techniques did not find more frequent use in neuroembryological research.


Dorsal Root Ganglion Chick Embryo Superior Cervical Ganglion Nonneuronal Cell Autonomic Neuron 



central nervous system


peripheral nervous system


spinal cord


superior cervical ganglion


dorsal root ganglion






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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Richard P. Bunge
    • 1
  1. 1.Department of AnatomyWashington University School of MedicineSt. LouisUSA

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