The Correlation between the Binding of the Human Chorionic Gonadotropin to Luteal Cells and Plasma Membrane of Luteal Cells

  • S. Papaionannou
  • D. Gospodarowicz


Granulosa cells as well as luteal cells maintained in tissue culture require a low but constant level of LH or hCG to stay differentiated (1,2).It can therefore be assumed that all factors regulating the appearance or disappearance (modulation) of the receptor sites for gonadotropins are of importance for the luteotrophic function and the differentiation of luteal cells. To study the modulation of the receptor sites in luteal cells maintained in tissue culture, one needed a binding technique specific enough to detect small variations in receptor concentration under different culture conditions. Also, since luteal cells are obtained by enzymatic digestion, and since it had been shown that proteolytic enzymes can destroy receptors (3–7), one must be assured that such a treatment does not significantly alter the characteristics of the binding of gonadotropins to their receptors. In this communication we compare the binding characteristics of hCG to bovine luteal membranes and bovine luteal cells obtained by enzymatic treatment (7). We have been able to show that the binding was identical and that the enzyme treatment needed to dissociate the luteal cells before putting them into culture did not affect the receptor site for gonadotropins.


Granulosa Cell Specific Binding Chorionic Gonadotropin Human Chorionic Gonadotropin Receptor Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Channing, C.P., Endocrinology 94: 1215, 1974.PubMedCrossRefGoogle Scholar
  2. 2.
    Gospodarowicz, D. and F.Gospodarowicz, Endocrinology submitted for publication.Google Scholar
  3. 3.
    Gospodarowicz, D., J Biol Chem 248: 5057, 1973Google Scholar
  4. 4.
    Rao, C.H.V., J Biol Chem 249: 2864, 1974.PubMedGoogle Scholar
  5. 5.
    Haour, F. and B. B. Saxena, J Biol Chem 249: 2195, 1974.PubMedGoogle Scholar
  6. 6.
    Lee, C. Y., and R. Ryan, Pro Natl Acad Sci USA 69: 3 5 20, 197 2.Google Scholar
  7. 7.
    Gospodarowicz, D., and F. Gospodarowicz, Endocrinology 90: 1427, 1972.PubMedCrossRefGoogle Scholar
  8. 8.
    Papkoff,H., D.Gospodarowicz, and C.H.Li, Arch Biochem Biophys III: 431, 1965.Google Scholar
  9. 9.
    Papkoff, H., D. Gospodarowicz, A. Candiotti, and C.H.Li, Arch Biochem Biophys III: 431, 1965.Google Scholar
  10. 10.
    Pierce, J.G., and M. E. Carsten, IN Werner, S.G. and C.C.Thomas (eds.) Thyrotropin.Charles C.Thomas,Springfield, Illinois, 1963, p. 216.Google Scholar
  11. 11.
    Gospodarowicz, D., J Biol Chem 248: 5050, 1973.PubMedGoogle Scholar
  12. 12.
    Lowry, O.H., N.J. Rosenbrough,A.L.Farr, and R.J.Randall, J Biol Chem 193: 265, 1951.PubMedGoogle Scholar
  13. 13.
    Cuatrecasas, P., Proc Natl Adad Sci USA 68: 1264, 1971.CrossRefGoogle Scholar
  14. 14.
    Cuatrecasas, P., J Biol Chem 246: 7265, 1972.Google Scholar
  15. 15.
    Dufau, M. L., T. Turuhara, and K.J.Catt, Biochem Biophys Acta 278: 281– 292, 1972.Google Scholar
  16. 16.
    Miyachi,Y., J.L.Vaitukaitis,E.Nieschlag,and M.B.Lipsett,J Clin Endocrin Metab 34: 23, 1972Google Scholar
  17. 17.
    Kammerman, S., R.E. Canfield, J. Kolena, and C.P. Channing, Endocrinology, 91: 65, 1972.PubMedCrossRefGoogle Scholar
  18. 18.
    Lee, C.Y., R.J.Ryan, Biochemistry 12: 4609, 1973.PubMedCrossRefGoogle Scholar
  19. 19.
    Freychet, P., R. Kahn, J. Roth, D.M. Neville, Jr., J Biol Chem 247: 3953, 1972.PubMedGoogle Scholar
  20. 20.
    Pohl, S.L., H.M.J. Krans, L.Birnbaumer, M. Rodbell, J Biol Chem 247: 2295, 1972.PubMedGoogle Scholar
  21. 21.
    Petit, V.A., M. Edidin, Science 184: 1183, 1974.PubMedCrossRefGoogle Scholar
  22. 22.
    Leidenberger, F., L. E. Reichert, Jr., Endocrinology 92: 646, 1973.PubMedCrossRefGoogle Scholar
  23. 23.
    Clarke, J.T., Ann NY Adad Sci 121: 248, 1965.Google Scholar
  24. 24.
    Ward, S., D.L. Wilson, J.J. Gilliam, Anal Biochem 38: 90, 1970.PubMedCrossRefGoogle Scholar
  25. 25.
    Gospodarowicz, D., J Biol Chem 247: 6491, 1974.Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • S. Papaionannou
    • 1
  • D. Gospodarowicz
    • 1
  1. 1.The Salk Institute for Biological StudiesSan DiegoUSA

Personalised recommendations