Nuclear-Cytoplasmic Interactions in the Biogenesis of Mitochondria in Yeast

  • Ronald A. Butow
  • William F. Bennett
  • David B. Finkelstein
  • Rodney E. Kellems


Studies on the biogenesis of mitochondria have now provided a reasonable evaluation of the degree of mitochondrial autonomy within the cell. Although mitochondria are capable of replicating, transcribing, and translating their genome as processes intrinsic to the organelle, these activities, nevertheless, have an absolute dependence upon macromolecules of extramitochondrial origin encoded by nuclear genes.1–3 It is also evident that the continuity of junctional mitochondria requires the complete, or nearly complete, expression of the mitochondrial genome, for interruption not only of the replication, but the transcription or translation of mitochondrial DNA (mtDNA) as well, leads to an impairment of mitochondrial activities.4–8 In the case of yeast, we know that this impairment results in the elaboration of a respiratory-deficient phenotype. Considering the extent of mitochondrial dependence on products encoded by nuclear genes, it would be surprising if the mitochondrial and the mitochondria-related nuclear-cytoplasmic systems which function in the development and persistence of the organelle were not subject to some types of coordinate regulation. A full understanding of the extent of regulation requires information on the products contributed by the mitochondrial and nuclear-cytoplasmic systems and the manner in which these products are brought together during mitochondrial development.


Mitochondrial Protein Outer Mitochondrial Membrane Mitochondrial Density Cytochrome Oxidase Activity Mitochondrial Protein Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Beattie, D. S. (1971), Subcell. Biochem. 1:1.Google Scholar
  2. 2.
    Linnane, A. W., Haisam, J. M., Lukins, H. B., and Nagley, P. (1972), Ann. Rev. Microbiol. 26:163.CrossRefGoogle Scholar
  3. 3.
    Borst, P. (1972), Ann. Rev. Biochem. 41:333.PubMedCrossRefGoogle Scholar
  4. 4.
    Schatz, G. M. (1970), in: Membranes of Mitochondria and Chloroplasts (E. Racker, ed.), Van Nostrand Reinhold Company, New York, p. 251.Google Scholar
  5. 5.
    Fukuhara, H. and Kujawa, C. (1970), Biochem. Biophys. Res. Commun. 41:1002.PubMedCrossRefGoogle Scholar
  6. 6.
    Wintersberger, U. and Wintersberger, E. (1970), Eur. J. Biochem. 13:20.PubMedCrossRefGoogle Scholar
  7. 7.
    Weislogel, P. O. and Butow, R. A. (1971), J. Biol. Chem. 246:5113.PubMedGoogle Scholar
  8. 8.
    Clark-Walker, G. D. and Linnane, A. W. (1967), J. Cell. Biol. 34:1.PubMedCrossRefGoogle Scholar
  9. 9.
    Guthrie, C., Nashimoto, H., and Nomura, M. (1969), Proc. Natl. Acad. Sci. USA 63:384.PubMedCrossRefGoogle Scholar
  10. 10.
    Williamson, D. H., Maroudas, H. G., and Wilkie, D. (1971), Mol. Gen. Genet. 111:209.PubMedCrossRefGoogle Scholar
  11. 11.
    Carnevali, F., Leoni, L., Morpurgo, G., and Conti, G. (1971), Mutat. Res. 12:357.CrossRefGoogle Scholar
  12. 12.
    Wintersberger, U. and Hirsch, J. (1973), Molec. Gen. Genet. 126:61.PubMedCrossRefGoogle Scholar
  13. 13.
    Eisenstadt, J. and Lengyel, P. (1966), Science 154:524.PubMedGoogle Scholar
  14. 14.
    Bianchetti, R., Lucchini, G., and Sartirana, M. L. (1971), Biochem. Biophys. Res. Commun. 42:97.PubMedCrossRefGoogle Scholar
  15. 15.
    Wintersberger, W. and Hirsch, J. (1973), Molec. Gen. Genet. 126:71.PubMedCrossRefGoogle Scholar
  16. 16.
    Grossman, L. I., Goldring, E. S., and Marmur, J. (1969), J. Mol. Biol. 46:367.PubMedCrossRefGoogle Scholar
  17. 17.
    Kuzela, S. and Grecná, E. (1969), Experentia 25:776.CrossRefGoogle Scholar
  18. 18.
    Schatz, G. and Saltzgaber, J. (1969), Biochem. Biophys. Res. Commun. 37:996.PubMedCrossRefGoogle Scholar
  19. 19.
    Mason, T. L. and Schatz, G. (1973), J. Biol. Chem. 248:1355.PubMedGoogle Scholar
  20. 20.
    Sebald, W., Weiss, H., and Jackl, G. (1972), Eur. J. Biochem. 30:413.PubMedCrossRefGoogle Scholar
  21. 21.
    Ebner, E., Mason, T. L., and Schatz, G. (1973), J. Biol. Chem. 248:5369.PubMedGoogle Scholar
  22. 22.
    Tzagoloff, A. and Meagher, P. (1972),J. Biol. Chem. 247:594.PubMedGoogle Scholar
  23. 23.
    Perlman, P. S. and Mahler, A. R. (1971), Biochem. Biophys. Res. Commun. 44:261.PubMedCrossRefGoogle Scholar
  24. 24.
    Mahler, H. R. and Perlman, P. S. (1972), J. Supramol. Struct. 1:105.PubMedCrossRefGoogle Scholar
  25. 25.
    Hollenberg, C. P. and Borst, P. (1971), Biochem. Biophys. Res. Commun. 45:1250.PubMedCrossRefGoogle Scholar
  26. 26.
    Slonimski, P. P. (1953), Formation des Enzymes Respiratoires chez la Levure, Masson, Paris.Google Scholar
  27. 27.
    Groot, G. S. P., Rouslin, W., and Schatz, G. (1972),J. Biol. Chem. 247:1735.PubMedGoogle Scholar
  28. 28.
    Butow, R. A., Ferguson, M. J., and Cederbaum, A. (1973), Biochemistry 12:158.PubMedCrossRefGoogle Scholar
  29. 29.
    Sherman, F. (1963), Genetics 48:375.PubMedGoogle Scholar
  30. 30.
    Sherman, F. and Slonimski, P. P., (1964), Biochim. Biophys. Acta 90:1.PubMedCrossRefGoogle Scholar
  31. 31.
    Reilly, C. and Sherman, F. (1965), Biochim. Biophys. Acta 95:640.PubMedGoogle Scholar
  32. 32.
    Ebner, F., Mennuci, L., and Schatz, G. (1973), J. Biol. Chem. 248:5360.PubMedGoogle Scholar
  33. 33.
    Howley, E. S. and Greenawalt, J. W. (1970),J. Biol. Chem. 254:3574.Google Scholar
  34. 34.
    Schweyen, R. and Kaudewitz, F. (1970), Biochem. Biophys. Res. Commun. 38:728.PubMedCrossRefGoogle Scholar
  35. 35.
    Beattie, D. S. (1970), Fed. Eur. Biochem. Soc. Lett. 9:232.CrossRefGoogle Scholar
  36. 36.
    Rubin, M. S. and Tzagoloff, A. (1973), J. Biol. Chem. 248:4275.PubMedGoogle Scholar
  37. 37.
    Tzagoloff, A., Rubin, M. S., and Sierra, M. F. (1973), Biochim. Biophys. Acta. 301:71.PubMedGoogle Scholar
  38. 38.
    Weiss, H. and Ziganke, B. (1974), Eur. J. Biochem. 41:63.PubMedCrossRefGoogle Scholar
  39. 39.
    Kellerman, G. M., Griffiths, D. E., Hansby, J. E., Lanvb, A. J., and Linnane, A. W. (1971), in: Autonomy and Biogenesis of Mitochondria and Chloroplasts (N. K. Boardman, A. W. Linnane, and R. M. Smillie, eds.), North Holland, Amsterdam, p. 346.Google Scholar
  40. 40.
    Perlman, P. S. and Mahler, H. R. (1970),J. Bioenerget. 1:113.CrossRefGoogle Scholar
  41. 41.
    Wojtczak, L. and Faluksa, H. (1969), Biochim. Biophys. Acta 193:64.PubMedCrossRefGoogle Scholar
  42. 42.
    Luck, D. J. and Reich, E. (1964), Proc. Natl. Acad. Sci. USA 52:931.PubMedCrossRefGoogle Scholar
  43. 43.
    Gonzalez-Cadavid, N. F. and Campbell, P. N. (1967), Biochem. J. 105:443.PubMedGoogle Scholar
  44. 44.
    Freeman, K. B., Haider, D., and Work, T. S. (1967), Biochem. J. 105:947.PubMedGoogle Scholar
  45. 45.
    Davidson, N. and Penniall, R. (1969), Arch. Biochem. Biophys. 133:345.CrossRefGoogle Scholar
  46. 46.
    Kadenbach, B. (1970), Eur. J. Biochem. 12:5027.CrossRefGoogle Scholar
  47. 47.
    Tzagoloff, A. (1972),J. Bioenerget. 3:39.CrossRefGoogle Scholar
  48. 48.
    Campbell, P. N. (1970), FEBS Lett. 7:1.PubMedCrossRefGoogle Scholar
  49. 49.
    Porter, K. R. and Bonneville, M. A. (1968), in: Fine Structure of Cells and Tissue (3rd ed.), Lea and Febiger, Philadelphia, pp. 7–9.Google Scholar
  50. 50.
    Watson, K. (1972),J. Cell Biol. 55:721.PubMedCrossRefGoogle Scholar
  51. 51.
    Keyhani, E. (1973),J. Cell Biol. 58:480.PubMedCrossRefGoogle Scholar
  52. 52.
    Wood, D. D. and Luck, D. J. L. (1969),J. Mol. Biol. 41:211.PubMedCrossRefGoogle Scholar
  53. 53.
    Schäfer, K. P. and Küntzel, H. (1972), Biochem. Biophys. Res. Commun. 46:1312.PubMedCrossRefGoogle Scholar
  54. 54.
    Aloni, Y. and Attardi, G. (1971),J. Mol. Biol. 55:271.PubMedCrossRefGoogle Scholar
  55. 55.
    Dawid, I. B. (1972),J. Mol. Biol. 63:201.PubMedCrossRefGoogle Scholar
  56. 56.
    Rendi, R. (1959), Exp. Cell. Res. 18:187.PubMedCrossRefGoogle Scholar
  57. 57.
    Mager, J. (1960), Biochim. Biophys. Acta 38:150.PubMedCrossRefGoogle Scholar
  58. 58.
    Kellems, R. E. and Butow, R. A. (1972),J. Biol. Chem. 247:8043.PubMedGoogle Scholar
  59. 59.
    Penman, D., Fan, H., Perlman, S., Rosbach, S., Weinberg, R., and Zylber, E. (1970), Cold Spring Harbor Symp. Quant. Biol. 35:561.CrossRefGoogle Scholar
  60. 60.
    Finkelstein, D. B., Blamire, J., and Marmur, J. (1972), Nat. New Biol. 240:279.PubMedGoogle Scholar
  61. 61.
    de Kloet, S. R. (1973),J. Bacteriol. 114:1034.PubMedGoogle Scholar
  62. 62.
    Kaback, D. B., Bhargava, M. M., and Halvorson, H. O. (1973),J. Mol. Biol. 79:735.PubMedCrossRefGoogle Scholar
  63. 63.
    Fellner, P., Ehresmannn, C., Stiegler, P., and Ebel, J. P. (1972), Nat. New Biol. 239:1.PubMedCrossRefGoogle Scholar
  64. 64.
    Mirzabikow, A. D. and Griffin, B. E. (1972),J. Mol. Biol. 72:633.CrossRefGoogle Scholar
  65. 65.
    Kellems, R. E. and Butow, R. A. (1974),J. Biol. Chem. 249:3304.PubMedGoogle Scholar
  66. 66.
    Palade, G. E. (1958) in: Microsomal Particles and Protein Synthesis (R. T. Roberts, ed.), Permagon Press, Elmsford, New York, p. 36.Google Scholar
  67. 67.
    Birheck, M. S. C. and Mercer, E. H. (1961), Nature (Lond.) 189:558.CrossRefGoogle Scholar
  68. 68.
    Takagi, M. and Ogata, K. (1968), Biochem. Biophys. Res. Commun. 33:55.PubMedCrossRefGoogle Scholar
  69. 69.
    Redman, C. M. (1969),J. Biol. Chem. 244:4388.Google Scholar
  70. 70.
    Redman, C. M. and Sabatini, D. D. (1966), Proc. Natl. Acad. Sci. USA 56:608.PubMedCrossRefGoogle Scholar
  71. 71.
    Kellems, R. E., Allison, V. F., and Butow, R. A. (1974),J. Biol. Chem. 249:3297.PubMedGoogle Scholar
  72. 72.
    Andrews, T. M. and Tata, J. R. (1971), Biochem. J. 121:683.PubMedGoogle Scholar
  73. 73.
    Adelman, M. R., Sabatini, D. D., and Blobel, G. (1973),J. Cell Biol. 56:206.PubMedCrossRefGoogle Scholar
  74. 74.
    Chua, N., Blobel, G., Siekevitz, P., and Palade, G. E. (1973), Proc. Natl. Acad. Sci. USA 70:1554.PubMedCrossRefGoogle Scholar
  75. 75.
    Kuriyama, Y. and Luck, D. J. L. (1973),J. Cell Biol. 59:776.PubMedCrossRefGoogle Scholar
  76. 76.
    Loeb, J. N., Howell, R. R., and Tompkins, G. M. (1967),J. Biol. Chem. 242:2069.PubMedGoogle Scholar
  77. 77.
    Goldblatt, P. J. (1972), Sub-Cell. Biochem. 1:147.Google Scholar
  78. 78.
    Lee, S. Y., Krsmanovic, V., and Brawerman, G. (1971), J. Cell Biol. 44:683.CrossRefGoogle Scholar
  79. 79.
    Hackenbrock, C. R. (1968), Proc. Natl. Acad. Sci. USA 61:598.PubMedCrossRefGoogle Scholar
  80. 80.
    Watkins, W. M. and Hassid, W. Z. (1962),J. Biol. Chem. 237:1432.PubMedGoogle Scholar
  81. 81.
    Diegelmann, R. T., Bernstein, L., and Peterkofsky, B. (1973),J. Biol. Chem. 248:6514.PubMedGoogle Scholar
  82. 82.
    Olsen, B. R., Berg, R. A., Kishidy, Y., and Prockop, D. J. (1973), Science 182:825.PubMedCrossRefGoogle Scholar
  83. 83.
    Nihei, T. (1971), Biochem. Bjophys. Res. Commun. 43:1139.CrossRefGoogle Scholar
  84. 84.
    Tanaka, T. and Ogata, K. (1971),J. Biochem. (Tokyo) 70:693.Google Scholar
  85. 85.
    Rosbash, M. (1972),J. Mol. Biol. 65:413.PubMedCrossRefGoogle Scholar
  86. 86.
    Cohen, P. P. (1970), Science 168:533.PubMedCrossRefGoogle Scholar
  87. 87.
    Ruby, J. R., Dyer, R. F., and Shalko, R. G. (1969), Z. Zellforsch. Mikroskop. Anat. 97:30.CrossRefGoogle Scholar
  88. 88.
    Bracher, C. E. and Grove, S. N. (1971), Protoplasma 73:15.CrossRefGoogle Scholar
  89. 89.
    Franke, W. W. and Kartenbeck, J. (1971), Protoplasma 73:35.PubMedCrossRefGoogle Scholar
  90. 90.
    Moore, D. J., Merritt, W. D., and Lembi, C. A. (1971), Protoplasma 73:43.CrossRefGoogle Scholar
  91. 91.
    Tewari, J. P. and Malhotra, S. K. (1973), Sub-Cell. Biochem. 2:287.Google Scholar
  92. 92.
    Gonzalez-Cadavid, N. F. and Campbell, P. N. (1967), Biochem. J. 105:443.PubMedGoogle Scholar
  93. 93.
    Gonzalez-Cadavid, N. F., Brovo, M., and Campbell, P. N. (1968), Biochem. J. 107:523.PubMedGoogle Scholar
  94. 94.
    Godinot, C. and Lardy, H. A. (1973), Biochemistry 12:2051.PubMedCrossRefGoogle Scholar
  95. 95.
    Weislogel, P. O. and Butow, R. A. (1970), Proc. Natl. Acad. Sci. USA 67:52.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Ronald A. Butow
    • 1
  • William F. Bennett
    • 1
  • David B. Finkelstein
    • 1
  • Rodney E. Kellems
    • 1
  1. 1.The University of Texas Southwestern Medical SchoolDallasUSA

Personalised recommendations