Abstract
In this article we would like to outline the close cooperation between the mitochondrial and cytoplasmic protein-synthesizing systems in mitochondrial biogenesis. The subject of our studies is two proteins of the mitochondrial membrane, cytochrome oxidase and cytochrome b. We will give a concise survey of our own experimental results and of the concepts we have formed during recent years of investigation on the fungus Neurospora crassa. In this chapter we will describe first the procedures for selective labeling of mitochondrial proteins according to their site of biosynthesis (either the mitochondrial or the nucleocytoplasmic-translation system). Secondly, we will describe techniques for the separation of the radioactively-labeled mitochondrial membrane proteins and the subsequent purification of cytochrome oxidase and cytochrome b. We will report on a new chromatographic system for the purification of mitochondrial membrane proteins which we believe to be applicable to a wider range of biological membranes. We will then review results on the molecular structure and biogenesis of cytochrome oxidase and cytochrome b and conclude by discussing our results together with those of other groups with regard to mitochondrial biogenesis in general.
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References
Schwab, A. J., Sebald, W., and Weiss, H. (1972), Eur. J. Biochem. 30:511.
Pall, M. L. (1969), Biochim. Biophys. Acta 173:113.
Sebald, W., Schwab, A. J., and Bücher, Th. (1969), FEBS Lett. 4:243.
Hawley, E. S. and Greenawalt, J. W. (1970),J. Biol. Chem. 245:3574.
Sisler, H. D. and Siegel, M. R. (1967), in: Antibiotics, Mechanism of Action (D. Gottlieb and P. D. Shaw, eds.), Springer Verlag, Berlin, p. 283.
Clark-Walker, G. D. and Linnane, A. W. (1966), Biochem. Biophys. Res. Commun. 25:8.
Beattie, D. S., Basford, R. E., and Koritz, S. B., (1967), Biochemistry 6:3099.
Neupert, W., Bridiczka, R. E., and Bücher, Th. (1967), Biochem. Biophys. Res. Commun. 27:488.
Beattie, D. S. (1968),J. Biol. Chem. 243:4027.
Sebald, W., Hofstötter, Th., Hacker, D., and Bücher, Th. (1969), FEBS Lett. 2:177.
Schatz, G. and Saltzgaber, J. (1969), Biochem. Biophys. Res. Commun. 37:996.
Küntzel, H. (1969), Nature (Lond.) 222:142.
Sebald, W., Schwab, A. J., and Bücher, Th. (1969), in: Inhibitor-Tools in Cell Research (Th. Bücher and H. Sies, eds.), Springer Verlag, Berlin, p. 140.
Neupert, W. and Ludwig, G. D. (1971), Eur. J. Biochem. 19:523.
Schweyen, R. and Kaudewitz, F. (1971), Biochem. Biophys. Res. Commun. 44:1351.
Weiss, H., von Jagow, G., Klingenberg, M., and Bücher, Th. (1970), Eur. J. Biochem. 14:75.
Weiss, H. and Bücher, Th. (1970), Eur. J. Biochem. 17:561.
Chapman, D. (1964), The Structure of Lipids, Methuen, London.
Weiss, H., Sebald, W., and Bücher, Th. (1971), Eur. J. Biochem. 22:19.
Weiss, H. (unpublished experiments).
Weiss, H., Lorenz, B., and Kleinow, W. (1972), FEBS Lett. 25:49.
Weiss, H. (1972), Eur. J. Biochem. 30:469.
Weiss, H. and Ziganke, B. (1974), Eur. J. Biochem. 41:63.
Lorenz, B., Kleinow, W., and Weiss, H. (1974), Z. Physiol. Chem. 355:300.
Sebald, W., Machleidt, W., and Otto, J. (1973), Eur. J. Biochem. 38:311.
Smith, L. and Conrad, H. (1956), Arch. Biochem. Biophys. 63:403.
Yonetani, T. (1961),J. Biol. Chem. 2b36:1680.
Rubin, M. S. and Tzagoloff, A. (1973), J. Biol. Chem. 248:4269.
Sekuzu, J., Mizuskima, H., and Okunuki, K. (1964), Biochem. Biophys. Acta 85:516.
Mason, T. L., Poyton, R. O., Wharton, D. C., and Schatz, G. (1973), J. Biol. Chem. 248:1346.
Weber, K. and Osborn, M. (1969), J. Biol. Chem. 244:4406.
Sebald, W., Weiss, H., and Jackl, G. (1972), Eur. J. Biochem. 30:413.
Werner, S. (1974), Eur. J. Biochem. 43:39.
Tzagoloff, A., Rubin, M. S., and Sierra, M. F. (1973), Biochem. Biophys. Acta 301:71.
Ebner, E., Mason, T. L., and Schatz, G. (1973),J. Biol. Chem. 248:5369.
Werner, S., Schwab, A. J., and Neupert, W. (1974), Eur. J. Biochem. 49:607.
Schatz, G., Groot, G. S. P., Mason, T., Rouslin, W., Wharton, D. C., and Saltzgaber, J. (1972), Fed. Proc. Amer. Soc. Exp. Biol. 31:21.
Tzagoloff, A., Akai, A., and Rubin, M. S. (1974), in: The Biogenesis of Mitochondria (A. M. Kroon and C. Saccone, eds.), Academic Press, London, p. 405.
Weiss, H., Sebald, W., Schwab, A. J., Kleinow, W., and Lorenz, B. (1973), Biochemie 6–7:815.
Hübscher, G., Kiese, M., and Nicolas, R. (1954), Biochem. Z. 325:223.
Goldberger, R., Smith, A. L., Tisdale, H., and Bomstein, R. (1961), J. Biol. Chem. 236:2788.
Ohnishi, K. (1966), J. Biochem. (Tokyo) 59:1.
Weiss, H., Ziganke, B., and Kolb, H. (in preparation).
Svedberg, T., and Pederson, K. O. (1940), The Ultracentrifuge, Oxford Univ. Press.
Tanford, Ch., (1967), Physical Chemistry ojMacromolecules, Wiley, New York.
Weiss, H. and Ziganke, B. (1974), in: The Biogenesis of Mitochondria (A. M. Kroon and C. Saccone, eds.), Academic Press, London, p. 491.
Mason, T. and Schatz, G. (1973),J. Biol. Chem. 248:1355.
Rubin, M. S. and Tzagoloff, A. (1973), J. Biol. Chem. 248:4275.
Kroon, A. M. and Saccone, C. (1974), The Biogenesis of Mitochondria, Academic Press, London.
Slonimski, P. P. (1953), La Formation des Enzymes Respiratoires chez la Levure, Masson, Paris.
Ephrussi, B. and Slonimski, P. P. (1955) Nature (Lond.) 176:1207.
Mitchell, M. B. and Mitchell, H. K. (1952), Proc. Natl. Acad. Sci. USA 38:442.
Mitchell, M. B., Mitchell, H. K., and Tissieres, A. (1953), Proc. Nat. Acad. Sci. USA 39:606.
Clark-Walker, G. D. and Linnane, A. W. (1967), J. Cell. Biol. 34:1.
Chen, W. L. and Charalampous, F. C. (1969), J. Biol. Chem. 244:2767.
Borst, P. (1972), Ann. Rev. Bioch. 41:333.
Brosemer, R. W., Vogell, W., and Bücher, Th. (1963), Biochem. Z. 338:854.
Michel, R. and Neupert, W. (1973), Eur. J. Biochem. 36:53.
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Weiss, H., Schwab, A.J., Werner, S. (1975). Biogenesis of Cytochrome Oxidase and Cytochrome b in Neurospora crassa . In: Tzagoloff, A. (eds) Membrane Biogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2136-1_5
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DOI: https://doi.org/10.1007/978-1-4684-2136-1_5
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