Biosynthesis and Assembly of the Outer Membrane Proteins of Escherichia coli

  • Masayori Inouye


The envelope of Escherichia coli consists of two distinct membranes: the outer membrane and the inner or cytoplasmic membrane.1,2 The cell wall or the peptidoglycan layer is located between the two membranes.2 Both the outer and the cytoplasmic membranes show typical features in electron micrographs of a unit membrane having a thickness of about 75 Å and consisting of phospholipids and proteins3 (Fig. 1). The cytoplasmic membrane is known to have many important functions such as energy metabolism, active transport, and synthesis of lipids, peptidoglycan, and lipopolysaccharide. The functions of the outer membrane are still somewhat obscure. So far only one enzyme, a phospholipase, has been suggested to exist in the outer membrane.4 One possible function of the outer membrane is thought to be as a barrier against penetration of certain substances such as antibiotics and macromolecules (see review by Nikaido5). However, many other substances which are required for cell growth should freely pass through the outer membrane, since the active transport systems for these substances are located exclusively in the cytoplasmic membrane.6,7 This suggests the existence of passive diffusion pores in the outer membrane. The lipopolysaccharide, which exists exclusively in the outer membrane, has been shown not to provide hydrophilic diffusion pores.8


Outer Membrane Cytoplasmic Membrane Free Form Envelope Protein Outer Membrane Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    De Petris, S. (1967),J. Ultrastruct. Res. 19:45.PubMedCrossRefGoogle Scholar
  2. 2.
    Murry, R. G. E., Steed, P., and Elson, H. H. (1965), Can. J. Microbiol. 11:547.CrossRefGoogle Scholar
  3. 3.
    Osborn, M. J., Gander, J. E., Parisi, E., and Carson, J. (1972), J. Biol. Chem. 247:3962.PubMedGoogle Scholar
  4. 4.
    Scandella, C. J. and Kornberg, A. (1971), Biochemistry 10:4447.PubMedCrossRefGoogle Scholar
  5. 5.
    Nikaido, H. (1973), in: Bacterial Membranes and Walls, Vol. 1 (L. Leive, ed.), Marcel Dekker, New York, p. 131.Google Scholar
  6. 6.
    Sistron, W. R. (1958), Biochim. Biophys. Acta. 29:579.CrossRefGoogle Scholar
  7. 7.
    Kaback, H. R. (1970), Annu. Rev. Biochem. 39:561.PubMedCrossRefGoogle Scholar
  8. 8.
    Nikaido, H. and Nakae, T. (1973), J. Inj. Dis. 128:S30.CrossRefGoogle Scholar
  9. 9.
    Haller, I. and Henning, U. (1974), Proc. Nat. Acad. Sci. USA 71:2018.PubMedCrossRefGoogle Scholar
  10. 10.
    Osborn, M. J., Gander, J. E., and Parisi, E. (1972), J. Biol. Chem. 247:3973.PubMedGoogle Scholar
  11. 11.
    Lee, N. and Inouye, M. (1974), FEBS Letters 39:167.PubMedCrossRefGoogle Scholar
  12. 12.
    Schnaitman, C. A. (1970), J. Bacteriol. 104:890.PubMedGoogle Scholar
  13. 13.
    Inouye, M. and Yee, M. (1973), J. Bacteriol. 113:304.PubMedGoogle Scholar
  14. 14.
    Inouye, M. and Yee, M. (1972), J. Bacteriol 112:585.PubMedGoogle Scholar
  15. 15.
    Inouye, M. and Guthrie, J. P. (1969), Proc. Nat. Acad. Sci. USA 64:957.PubMedCrossRefGoogle Scholar
  16. 16.
    Inouye, M. and Pardee, A. B. (1970), J. Biol. Chem. 245:5813.PubMedGoogle Scholar
  17. 17.
    Hirashima, A., Childs, G., and Inouye, M. (1973), J. Mol. Biol. 79:373.PubMedCrossRefGoogle Scholar
  18. 18.
    Braun, V., Bosch, V., Hantke, K., and Schaller, K. (1974), Ann. N.Y. Acad. Sci. 235:66.PubMedCrossRefGoogle Scholar
  19. 19.
    Ames, G. F. (1974),J. Biol. Chem. 249:634.PubMedGoogle Scholar
  20. 20.
    Schnaitman, C. A. (1974), J. Bacteriol. 118:442, 454.PubMedGoogle Scholar
  21. 21.
    Chai, T. and Foulds, J. (1974),J. Mol. Biol. 85:465.PubMedCrossRefGoogle Scholar
  22. 22.
    Koplow, J. and Goldfine, H. (1974),J. Bacteriol. 117:527.PubMedGoogle Scholar
  23. 23.
    Rosenbusch, J. (1974), J. Biol. Chem. 249:8019.PubMedGoogle Scholar
  24. 24.
    Nakamura, K., Ostrovsky, D. N., Miyazawa, T., and Mizushima, S. (1974), Biochim. Biophys. Acta. 332:329.CrossRefGoogle Scholar
  25. 25.
    Henning, U., Höhn, B., and Sonntag, I. (1973), Eur. J. Biochem. 39:27.PubMedCrossRefGoogle Scholar
  26. 26.
    Ames, G. F., Spudich, E. N., and Nikaido, H. (1974), J. Bacteriol. 117:406.PubMedGoogle Scholar
  27. 27.
    Inouye, M., Shaw, J., and Shen, C. (1972), J. Biol. Chem. 247:8154.PubMedGoogle Scholar
  28. 28.
    Braun, V. and Rehn, K. (1969), Eur. J. Biochem. 10:426.PubMedCrossRefGoogle Scholar
  29. 29.
    Braun, V. and Sieglin, J. (1970), Eur. J. Biochem. 13:336.PubMedCrossRefGoogle Scholar
  30. 30.
    Braun, V. and Wolff, H. (1970), Eur. J. Biochem. 14:387.PubMedCrossRefGoogle Scholar
  31. 31.
    Inouye, M. (1974), Proc. Nat. Acad. Sci. USA 71:2396.PubMedCrossRefGoogle Scholar
  32. 32.
    Braun, V. and Wolff, H. (1973), FEBS Letters 34:77.PubMedCrossRefGoogle Scholar
  33. 33.
    Braun, V., Schaller, K., and Wolff, H. (1973), Biochim. Biophys. Acta 323:87.PubMedCrossRefGoogle Scholar
  34. 34.
    Sabet, S. F. and Schnaitman, C. A. (1973),J. Biol. Chem. 248:1797.PubMedGoogle Scholar
  35. 35.
    Randall-Hazelbauer, L. and Schwartz, M. (1973), J. Bacteriol. 116:1436.PubMedGoogle Scholar
  36. 36.
    Wu, M. and Heath, E. C. (1973), Proc. Nat. Acad. Sci. USA 70:2572.PubMedCrossRefGoogle Scholar
  37. 37.
    Vambutas, V. K. and Salton, M. R. J. (1970), Biochim. Biophys. Acta 203:83, 94.PubMedGoogle Scholar
  38. 38.
    Allen, D. W. and Zamecnick, P. C. (1962), Biochim. Biophys. Acta 55:865.PubMedCrossRefGoogle Scholar
  39. 39.
    Nathans, D. (1964), Proc. Nat. Acad. Sci. USA 51:585.PubMedCrossRefGoogle Scholar
  40. 40.
    Hirashima, A. and Inouye, M. (1973), Nature (Lond.) 242:405.CrossRefGoogle Scholar
  41. 41.
    Voynow, P. and Kurland, C. G. (1971), Biochemistry 10:517.PubMedCrossRefGoogle Scholar
  42. 42.
    Deusser, E. (1972), Mol. Gen. Genet. 119:249.PubMedCrossRefGoogle Scholar
  43. 43.
    Weber, H. J. (1972), Mol. Gen. Genet. 119:233.PubMedCrossRefGoogle Scholar
  44. 44.
    Birnbaum, L. S. and Kaplan, S. (1971), Proc. Nat. Acad. Sci. USA 68:925.PubMedCrossRefGoogle Scholar
  45. 45.
    Unger, M., Birnbaum, L. S., Kaplan, S. and Pfister, A. (1972), Mol. Gen. Genet. 119:377.PubMedGoogle Scholar
  46. 46.
    Kozak, M. and Nathans, D. (1972), J. Mol. Biol. 70:41.PubMedCrossRefGoogle Scholar
  47. 47.
    Lodish, H. F. and Nathan, D. G. (1972),J. Biol. Chem. 247:7822.PubMedGoogle Scholar
  48. 48.
    Schlessinger, D., Marchesi, V. T., and Kwan, B. C. K. (1965),J. Bacteriol. 90:456.PubMedGoogle Scholar
  49. 49.
    Yudkin, M. D. and Davis, B. (1965),J. Mol. Biol. 12:193.PubMedCrossRefGoogle Scholar
  50. 50.
    Rouvière, J., Lederberg, S., Granboulan, P., and Gros, F. (1969),J. Mol. Biol. 46:413.PubMedCrossRefGoogle Scholar
  51. 51.
    Brown, D. G. and Abrams, A. (1970), Biochim. Biophys. Acta 200:522.PubMedGoogle Scholar
  52. 52.
    Cundliffe, E. (1970),J. Mol. Biol. 52:467.PubMedCrossRefGoogle Scholar
  53. 53.
    van Knippenberg, P. H., Diujts, G. A. H., and Euwe, M. S. T. (1971), Mol. Gen. Genet. 112:197.PubMedGoogle Scholar
  54. 54.
    van Knippenberg, P. H. and Duijts, G. A. H. (1971), Mol. Gen. Genet. 112:208.PubMedGoogle Scholar
  55. 55.
    Varricchio, F. (1972),J. Bacteriol. 109:1284.PubMedGoogle Scholar
  56. 56.
    Cancadda, R. and Schlesinger, M. J. (1974),J. Bacteriol. 117:290.Google Scholar
  57. 57.
    Groner, Y., Pollack, Y., Berissi, H., and Revel, M. (1972), Nat. New Biol. 239:16.PubMedGoogle Scholar
  58. 58.
    Both, G. W., Mclnnes, J. L., Hanlon, J. E., May, B. K., and Elliott, W. A. (1972),J. Mol. Biol. 67:199.PubMedCrossRefGoogle Scholar
  59. 59.
    Mühlradt, P. F., Menzel, J., Golecki, J. R., and Speth, V. (1973), Eur. J. Biochem. 35:471.PubMedCrossRefGoogle Scholar
  60. 60.
    Braun, V. and Bosch, V. (1972), Proc. Nat. Acad. Sci. USA 69:970.PubMedCrossRefGoogle Scholar
  61. 61.
    Braun, V. and Bosch, V. (1972), Eur. J. Biochem. 28:51.PubMedCrossRefGoogle Scholar
  62. 62.
    Hantke, K. and Braun, V. (1973), Eur. J. Biochem. 34:284.PubMedCrossRefGoogle Scholar
  63. 63.
    Hirashima, A., Wu, H. C., Venkateswaran, P. S., and Inouye, M. (1973),J. Biol. Chem. 248:5654.PubMedGoogle Scholar
  64. 64.
    Inouye, S., Takeishi, K., Hirashima, A., and Inouye, M. (manuscript in preparation).Google Scholar
  65. 65.
    Izaki, K., Matsuhashi, M., and Strominger, J. L. (1966), Proc. Nat. Acad. Sci. USA 55:656.PubMedCrossRefGoogle Scholar
  66. 66.
    Halegoua, S. and Inouye, M. (unpublished data).Google Scholar
  67. 67.
    Braun, V. and Bosch, V. (1973), FEBS Letters 34:302.PubMedCrossRefGoogle Scholar
  68. 68.
    Hirashima, A., Wang, S., and Inouye, M. (1974), Proc. Nat. Acad. Sci. USA 71:4149.PubMedCrossRefGoogle Scholar
  69. 69.
    Hirashima, A. and Inouye, M. (unpublished data).Google Scholar
  70. 70.
    Maizels, N. M. (1973), Proc. Nat. Acad. Sci. USA 70:3585.PubMedCrossRefGoogle Scholar
  71. 71.
    Bronson, M. J., Squires, C., and Yanofsky, C. (1973), Proc. Nat. Acad. Sci. USA 70:2335.PubMedCrossRefGoogle Scholar
  72. 72.
    Braun, V. (1974), J. Inf. Dis. 128:S9.CrossRefGoogle Scholar
  73. 73.
    Lacroute, F. and Stent, G. S. (1968),J. Mol. Biol. 35:165.PubMedCrossRefGoogle Scholar
  74. 74.
    Forchhammer, J. and Lindahl, L. (1971), J. Mol. Biol. 55:563.PubMedCrossRefGoogle Scholar
  75. 75.
    Gausing, K. (1972), J. Mol. Biol. 71:529.PubMedCrossRefGoogle Scholar
  76. 76.
    Bosch, V. and Braun, V. (1973), FEBS Letters 34:307.PubMedCrossRefGoogle Scholar
  77. 77.
    Hodges, R. S., Sodek, J., Smillie, L. B., and Jurasek, L. (1972), Cold Spring Harbor Symp. Quant. Biol. 37:299.CrossRefGoogle Scholar
  78. 78.
    Braun, V., Gnike, H., Henning, U., and Rehn, K. (1973),J. Bacteriol. 114:1264.PubMedGoogle Scholar
  79. 79.
    DeMartini, M. and Inouye, M. (unpublished data).Google Scholar
  80. 80.
    Decad, G., Nakae, T., and Nikaido, H. (1974), Fed. Proc. 33:1240.Google Scholar
  81. 81.
    Halegoua, S., Hirashima, A., and Inouye, M. (1974), J. Bacteriol. 120:1204.PubMedGoogle Scholar
  82. 82.
    Braun, V., Rehn, K., and Wolff, H. (1970), Biochemistry 9:5041.PubMedCrossRefGoogle Scholar
  83. 83.
    Henning, U., Rehn, K., and Hoehn, B. (1973), Proc. Nat. Acad. Sci. USA 70:2033.PubMedCrossRefGoogle Scholar
  84. 84.
    Leive, L. (1965), Biochim. Biophys. Res. Commun. 18:13.CrossRefGoogle Scholar
  85. 85.
    Voll, M. J. and Leive, L. (1970), J. Biol. Chem. 245:1108.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Masayori Inouye
    • 1
  1. 1.Department of BiochemistryState University of New York at Stony BrookStony BrookUSA

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