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In Vitro Studies of the gam Gene Product of Bacteriophage λ

  • Alexander Karu
  • Yoshiyuke Sakaki
  • Harrison Echols
  • Stuart Linn

Abstract

About six years have elapsed since it was established that the recB and recC genes of Escherichia coli specify a complex DNase-ATPase, the RecBC enzyme. Although this enzyme has been extensively purified, and a great deal is known of its catalytic properties and its preference for certain forms of DNA in vitro, its biochemical activity in vivo is still unknown. However, considerable evidence has accumulated indicating that this enzyme is antagonistic to the normal pathway for phage λ DNA replication, and that as a consequence, phage λ has evolved a means to regulate the RecBC enzyme.

Keywords

Bacteriophage Lambda Permissive Temperature Nonpermissive Temperature Bromphenol Blue Glycerol Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Alexander Karu
    • 1
  • Yoshiyuke Sakaki
    • 1
  • Harrison Echols
    • 1
  • Stuart Linn
    • 1
  1. 1.Department of Biochemistry and Department of Molecular BiologyUniversity of CaliforniaBerkeleyUSA

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