Recombination of Phage λ DNA in Vitro
Elucidation of the mechanism of genetic recombination at the biochemical level has been hampered by the paucity of recombination-related activities that can be measured in vitro. A major difficulty has been the lack of a direct and simple assay for recombinant DNA. I would like to describe conditions in which phage λ DNA recombines in vitro and the recombinant DNA is assayed following its assembly into infectious λ particles.
KeywordsCold Spring Harbor Genetic Recombination Indicator Bacterium Equilibrium Gradient Genetic Crossover
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