Recombination of Phage λ DNA in Vitro

  • Michael Syvanen


Elucidation of the mechanism of genetic recombination at the biochemical level has been hampered by the paucity of recombination-related activities that can be measured in vitro. A major difficulty has been the lack of a direct and simple assay for recombinant DNA. I would like to describe conditions in which phage λ DNA recombines in vitro and the recombinant DNA is assayed following its assembly into infectious λ particles.


Cold Spring Harbor Genetic Recombination Indicator Bacterium Equilibrium Gradient Genetic Crossover 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Michael Syvanen
    • 1
  1. 1.Department of BiochemistryStanford University School of MedicineStanfordUSA

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