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A Replicator’s View of Recombination (and Repair)

  • Ann Skalka

Abstract

During the course of studies on the growth of gamma λ mutants in rec + and rec - cells, L. W. Enquist and I (Enquist and Skalka, 1973) observed a growth defect in λ red mutants that was independent of the rec condition of the host. Red mutants synthesize their DNA at only 1/3 to 1/2 the wild-type rate, and concatemers formed at late times are on the average shorter than normal. Red - DNA also seems to be packaged with somewhat less than the wild-type efficiency, and phage bursts are only about 30 to 40% that of wild type (Table 1). The importance of red genes to λ growth is further emphasized by the occurrence of a number of bacterial mutants on which λ red mutants do not plate at all. This class, called feb - , includes polA- and lig - strains (Zissler et al., 1971). The following section summarizes results from studies of DNA synthesis and phage production in red - and gam - infection of these feb - hosts.

Keywords

Burst Size Rolling Circle Late Replication Synaptic Complex Phage Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Ann Skalka
    • 1
  1. 1.Roche Institute of Molecular BiologyNutleyUSA

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