Synaptonemal Complex Karyotyping in Spreads of Mammalian Spermatocytes

  • Montrose J. Moses
  • Sheila J. Counce

Abstract

The synaptonemal complex is a concomitant of meiotic chromosome synapsis and crossing-over in eukaryotes (Moses, 1969), and as such could serve as the basis for bivalent karyotype analysis at a higher level of resolution than hitherto possible. For example, Gillies (1972), on Neurospora, Moens (1974), on Locusta spermatocytes, and Carpenter (this volume), on Drosophila oocytes, are already realizing this potential from three-dimensional reconstructions of electron micrographs and serial sections. Moens and Perkins (1969) have used the method to establish the chromosome number of a protist. We have recently begun to apply karyotype analysis of these complexes to mammalian meiosis, using a new and somewhat less tedious technique.

Keywords

Formalin Recombination Phospho Metacen 

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References

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Montrose J. Moses
    • 1
  • Sheila J. Counce
    • 1
  1. 1.Departments of Anatomy and ZoologyDuke University Medical CenterDurhamUSA

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